Polyphasic characterization of bacillus strains isolated from maize.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126911 https://doi.org/10.18512/rbms2020v19e1190 |
Resumo: | The use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subtilis (B2082 and B2084), using 16S rRNA gene sequencing, MALDI-TOF mass spectrometry and/or protein crystal detection. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are the same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants quality control, strains traceability and to detail PGPB colonization. |
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Polyphasic characterization of bacillus strains isolated from maize.Perfil molecularCrescimento de plantasBactériaZea MaysThe use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subtilis (B2082 and B2084), using 16S rRNA gene sequencing, MALDI-TOF mass spectrometry and/or protein crystal detection. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are the same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants quality control, strains traceability and to detail PGPB colonization.UBIRACI GOMES DE PAULA LANA, CNPMSMARINA ELEUTÉRIO GÓI DE MORAES RODRIGUES, Centro Universitário de Sete Lagoas - UNIFEMMCHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMSSYLVIA MORAIS DE SOUSA TINOCO, CNPMSAMANDA NAYÊ GUIMARÃES TAVARESIVANILDO EVODIO MARRIEL, CNPMSELIANE APARECIDA GOMES, CNPMS.LANA, U. G. de P.RODRIGUES, M. E. G. de M.OLIVEIRA-PAIVA, C. A.SOUSA, S. M. deTAVARES, A. N. G.MARRIEL, I. E.GOMES, E. A.2020-11-27T09:01:12Z2020-11-27T09:01:12Z2020-11-222020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Milho e Sorgo, v. 19, e1190, 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126911https://doi.org/10.18512/rbms2020v19e1190enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-11-27T09:01:25Zoai:www.alice.cnptia.embrapa.br:doc/1126911Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-11-27T09:01:25falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-11-27T09:01:25Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Polyphasic characterization of bacillus strains isolated from maize. |
title |
Polyphasic characterization of bacillus strains isolated from maize. |
spellingShingle |
Polyphasic characterization of bacillus strains isolated from maize. LANA, U. G. de P. Perfil molecular Crescimento de plantas Bactéria Zea Mays |
title_short |
Polyphasic characterization of bacillus strains isolated from maize. |
title_full |
Polyphasic characterization of bacillus strains isolated from maize. |
title_fullStr |
Polyphasic characterization of bacillus strains isolated from maize. |
title_full_unstemmed |
Polyphasic characterization of bacillus strains isolated from maize. |
title_sort |
Polyphasic characterization of bacillus strains isolated from maize. |
author |
LANA, U. G. de P. |
author_facet |
LANA, U. G. de P. RODRIGUES, M. E. G. de M. OLIVEIRA-PAIVA, C. A. SOUSA, S. M. de TAVARES, A. N. G. MARRIEL, I. E. GOMES, E. A. |
author_role |
author |
author2 |
RODRIGUES, M. E. G. de M. OLIVEIRA-PAIVA, C. A. SOUSA, S. M. de TAVARES, A. N. G. MARRIEL, I. E. GOMES, E. A. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
UBIRACI GOMES DE PAULA LANA, CNPMS MARINA ELEUTÉRIO GÓI DE MORAES RODRIGUES, Centro Universitário de Sete Lagoas - UNIFEMM CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS SYLVIA MORAIS DE SOUSA TINOCO, CNPMS AMANDA NAYÊ GUIMARÃES TAVARES IVANILDO EVODIO MARRIEL, CNPMS ELIANE APARECIDA GOMES, CNPMS. |
dc.contributor.author.fl_str_mv |
LANA, U. G. de P. RODRIGUES, M. E. G. de M. OLIVEIRA-PAIVA, C. A. SOUSA, S. M. de TAVARES, A. N. G. MARRIEL, I. E. GOMES, E. A. |
dc.subject.por.fl_str_mv |
Perfil molecular Crescimento de plantas Bactéria Zea Mays |
topic |
Perfil molecular Crescimento de plantas Bactéria Zea Mays |
description |
The use of bioinoculants with plant growth-promoting bacteria (PGPB) is a sustainable agricultural practice that has been expanding worldwide. Thus, it is fundamental to characterize these bacteria molecularly to provide better security and traceability for bioinoculants production. This work aimed to identify, characterize and develop specific molecular markers for Bacillus strains related to plant-growing promotion. Five strains were identified as B. pumillus (B32), B. thuringiensis (B116), B. megaterium (B119) and B. subtilis (B2082 and B2084), using 16S rRNA gene sequencing, MALDI-TOF mass spectrometry and/or protein crystal detection. Repetitive element palindromic (Rep-PCR) and amplified ribosomal DNA restriction analysis (ARDRA) techniques allowed the discrimination of the strains, except B2082 and B2084 that presented identical genetic profiles, indicating that they are the same or genetically close isolates. Moreover, specific molecular markers were developed for B116 and B119 strains and may be used for microbial inoculants quality control, strains traceability and to detail PGPB colonization. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-27T09:01:12Z 2020-11-27T09:01:12Z 2020-11-22 2020 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Milho e Sorgo, v. 19, e1190, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126911 https://doi.org/10.18512/rbms2020v19e1190 |
identifier_str_mv |
Revista Brasileira de Milho e Sorgo, v. 19, e1190, 2020. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1126911 https://doi.org/10.18512/rbms2020v19e1190 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503498240360448 |