Calli induction in leaf explants of coffee elite genotypes.

Detalhes bibliográficos
Autor(a) principal: REZENDE, J. C. de
Data de Publicação: 2011
Outros Autores: CARVALHO, C. H. S. de, PASQUAL, M., SANTOS, A. C. R., CARVALHO, S. M. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/902616
Resumo: Three experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL & BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20μM) and 2-iP (2.5 e 20μM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under darkness, at 25±2oC. The medium described by TEIXEIRA et al (2004) was found to be superior when compared to that described by BOXTEL & BERTHOULY (1996) in the 2.2 and 7.2 genotypes. An opposite behavior was noticed in 4.2 genotype, that is, BOXTEL & BERTHOULY (1996) had medium superiority. Both 3.0 and 5.0 genotypes had the same behavior in both media studied, which shows that the somatic embryo production depends on the genotype. Calli induction depends on the 2-iP and 2.4 D ratio. The 20.0μM of 2.4-D and 20.0μM of 2-iP combination caused the highest embryogenic calli induction rate.
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spelling Calli induction in leaf explants of coffee elite genotypes.Growh regulatorsCoffea ArábicaThree experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL & BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20μM) and 2-iP (2.5 e 20μM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under darkness, at 25±2oC. The medium described by TEIXEIRA et al (2004) was found to be superior when compared to that described by BOXTEL & BERTHOULY (1996) in the 2.2 and 7.2 genotypes. An opposite behavior was noticed in 4.2 genotype, that is, BOXTEL & BERTHOULY (1996) had medium superiority. Both 3.0 and 5.0 genotypes had the same behavior in both media studied, which shows that the somatic embryo production depends on the genotype. Calli induction depends on the 2-iP and 2.4 D ratio. The 20.0μM of 2.4-D and 20.0μM of 2-iP combination caused the highest embryogenic calli induction rate.EPAMIG; CARLOS HENRIQUE S DE CARVALHO, SAPC; Universidade Federal de Lavras; CEIS.REZENDE, J. C. deCARVALHO, C. H. S. dePASQUAL, M.SANTOS, A. C. R.CARVALHO, S. M. de2011-10-06T11:11:11Z2011-10-06T11:11:11Z2011-10-06T11:11:11Z2011-10-06T11:11:11Z2011-10-0620112011-10-06T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCiência Rural, Santa Maria, v.41, n.3, p. 384-389, mar, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/902616enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:16:16Zoai:www.alice.cnptia.embrapa.br:doc/902616Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:16:16falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:16:16Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Calli induction in leaf explants of coffee elite genotypes.
title Calli induction in leaf explants of coffee elite genotypes.
spellingShingle Calli induction in leaf explants of coffee elite genotypes.
REZENDE, J. C. de
Growh regulators
Coffea Arábica
title_short Calli induction in leaf explants of coffee elite genotypes.
title_full Calli induction in leaf explants of coffee elite genotypes.
title_fullStr Calli induction in leaf explants of coffee elite genotypes.
title_full_unstemmed Calli induction in leaf explants of coffee elite genotypes.
title_sort Calli induction in leaf explants of coffee elite genotypes.
author REZENDE, J. C. de
author_facet REZENDE, J. C. de
CARVALHO, C. H. S. de
PASQUAL, M.
SANTOS, A. C. R.
CARVALHO, S. M. de
author_role author
author2 CARVALHO, C. H. S. de
PASQUAL, M.
SANTOS, A. C. R.
CARVALHO, S. M. de
author2_role author
author
author
author
dc.contributor.none.fl_str_mv EPAMIG; CARLOS HENRIQUE S DE CARVALHO, SAPC; Universidade Federal de Lavras; CEIS.
dc.contributor.author.fl_str_mv REZENDE, J. C. de
CARVALHO, C. H. S. de
PASQUAL, M.
SANTOS, A. C. R.
CARVALHO, S. M. de
dc.subject.por.fl_str_mv Growh regulators
Coffea Arábica
topic Growh regulators
Coffea Arábica
description Three experiments were carried out with the objective of achieving high effectiveness in calli induction from high heterozygosis leaf explants of Coffea arabica through indirect somatic embryogenesis. A randomized-block design in a 2x5 factorial arrangement made up of two media [BOXTEL & BERTHOULY (1996) and TEIXEIRA et al. (2004)] and five C. arabica genotypes were used in the first experiment. In the second experiment the embryogenic calli production potential was evaluated in ten genotypes. Each of them was considered as a treatment. In the third experiment the variations in both 2.4-D (2.5 e 20μM) and 2-iP (2.5 e 20μM) concentrations in TEIXEIRA et al. (2004) medium and secondary media were evaluated. Crops were kept in a growth room under darkness, at 25±2oC. The medium described by TEIXEIRA et al (2004) was found to be superior when compared to that described by BOXTEL & BERTHOULY (1996) in the 2.2 and 7.2 genotypes. An opposite behavior was noticed in 4.2 genotype, that is, BOXTEL & BERTHOULY (1996) had medium superiority. Both 3.0 and 5.0 genotypes had the same behavior in both media studied, which shows that the somatic embryo production depends on the genotype. Calli induction depends on the 2-iP and 2.4 D ratio. The 20.0μM of 2.4-D and 20.0μM of 2-iP combination caused the highest embryogenic calli induction rate.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-06T11:11:11Z
2011-10-06T11:11:11Z
2011-10-06T11:11:11Z
2011-10-06T11:11:11Z
2011-10-06
2011
2011-10-06T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Ciência Rural, Santa Maria, v.41, n.3, p. 384-389, mar, 2011.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/902616
identifier_str_mv Ciência Rural, Santa Maria, v.41, n.3, p. 384-389, mar, 2011.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/902616
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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