Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/978629 |
Resumo: | The use of peanut as a trap-plant for obtaining rhizobia may result in a high diversity of efficient isolates. This study aimed to evaluate the diversity and symbiotic efficiency of peanut rhizobia from the Southeastern region of Brazil. The bacteria were isolated from nodules of two genotypes in soils from the States of São Paulo and Rio de Janeiro, Brazil. The bacteria were authenticated and evaluated regarding to their symbiotic capacity. The rhizobial diversity was evaluated through their culture characteristics on yeast mannitol agar (YMA) culture media and by polymerase chain reaction Box- (PCR). Two selected isolates were characterized phenotypically regarding their capacity to solubilize phosphate, to grow on media with different concentrations of NaCI, pH's and incubation temperatures. For these the 16S rRNA gene sequencing and symbiotic efficiency using non-autoclaved soils were conducted. The majority of the obtained isolates showed rapid growth and acidified the culture medium. Analysis of the isolates through the Box-PCR revealed low similarity to the reference strain indicated for the culture and a large genetic variability of the obtained isolates. The isolates AM 01 and AM 07, which clustered with Bradyrhizobium and Rhizobium genus, respectively, showed a potential for evaluations and a means to recommend new rhizobia strains for peanut. |
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Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil.RizóbioFixação biológica de notrogênioPeanutAmendoimArachis HypogaeaThe use of peanut as a trap-plant for obtaining rhizobia may result in a high diversity of efficient isolates. This study aimed to evaluate the diversity and symbiotic efficiency of peanut rhizobia from the Southeastern region of Brazil. The bacteria were isolated from nodules of two genotypes in soils from the States of São Paulo and Rio de Janeiro, Brazil. The bacteria were authenticated and evaluated regarding to their symbiotic capacity. The rhizobial diversity was evaluated through their culture characteristics on yeast mannitol agar (YMA) culture media and by polymerase chain reaction Box- (PCR). Two selected isolates were characterized phenotypically regarding their capacity to solubilize phosphate, to grow on media with different concentrations of NaCI, pH's and incubation temperatures. For these the 16S rRNA gene sequencing and symbiotic efficiency using non-autoclaved soils were conducted. The majority of the obtained isolates showed rapid growth and acidified the culture medium. Analysis of the isolates through the Box-PCR revealed low similarity to the reference strain indicated for the culture and a large genetic variability of the obtained isolates. The isolates AM 01 and AM 07, which clustered with Bradyrhizobium and Rhizobium genus, respectively, showed a potential for evaluations and a means to recommend new rhizobia strains for peanut.CARLOS VERGARA TORRES JÚNIOR, UFRRJ; JAKSON LEITE, UFRPE; CAROLINA ETIENNE DE ROSÁLIA E SILVA SANTOS, UFRPE; PAULO IVAN FERNANDES JUNIOR, CPATSA; JERRI EDSON ZILLI, CNPAB; NORMA GOUVEA RUMJANEK, CNPAB; GUSTAVO RIBEIRO XAVIER, CNPAB.TORRES JÚNIOR, C. V.LEITE, J.SANTOS, C. E. de R. e S.FERNANDES JUNIOR, P. I.ZILLI, J. E.RUMJANEK, N. G.XAVIER, G. R.2014-02-05T11:11:11Z2014-02-05T11:11:11Z2014-02-0520142015-03-26T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAfrican Journal of Microbiology Research, v. 8 n. 6, p. 566-577, fev. 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/978629enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:45:57Zoai:www.alice.cnptia.embrapa.br:doc/978629Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:45:57falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:45:57Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil. |
title |
Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil. |
spellingShingle |
Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil. TORRES JÚNIOR, C. V. Rizóbio Fixação biológica de notrogênio Peanut Amendoim Arachis Hypogaea |
title_short |
Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil. |
title_full |
Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil. |
title_fullStr |
Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil. |
title_full_unstemmed |
Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil. |
title_sort |
Diversity and symbiotic performance of peanut rhizobia from Southeast region of Brazil. |
author |
TORRES JÚNIOR, C. V. |
author_facet |
TORRES JÚNIOR, C. V. LEITE, J. SANTOS, C. E. de R. e S. FERNANDES JUNIOR, P. I. ZILLI, J. E. RUMJANEK, N. G. XAVIER, G. R. |
author_role |
author |
author2 |
LEITE, J. SANTOS, C. E. de R. e S. FERNANDES JUNIOR, P. I. ZILLI, J. E. RUMJANEK, N. G. XAVIER, G. R. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
CARLOS VERGARA TORRES JÚNIOR, UFRRJ; JAKSON LEITE, UFRPE; CAROLINA ETIENNE DE ROSÁLIA E SILVA SANTOS, UFRPE; PAULO IVAN FERNANDES JUNIOR, CPATSA; JERRI EDSON ZILLI, CNPAB; NORMA GOUVEA RUMJANEK, CNPAB; GUSTAVO RIBEIRO XAVIER, CNPAB. |
dc.contributor.author.fl_str_mv |
TORRES JÚNIOR, C. V. LEITE, J. SANTOS, C. E. de R. e S. FERNANDES JUNIOR, P. I. ZILLI, J. E. RUMJANEK, N. G. XAVIER, G. R. |
dc.subject.por.fl_str_mv |
Rizóbio Fixação biológica de notrogênio Peanut Amendoim Arachis Hypogaea |
topic |
Rizóbio Fixação biológica de notrogênio Peanut Amendoim Arachis Hypogaea |
description |
The use of peanut as a trap-plant for obtaining rhizobia may result in a high diversity of efficient isolates. This study aimed to evaluate the diversity and symbiotic efficiency of peanut rhizobia from the Southeastern region of Brazil. The bacteria were isolated from nodules of two genotypes in soils from the States of São Paulo and Rio de Janeiro, Brazil. The bacteria were authenticated and evaluated regarding to their symbiotic capacity. The rhizobial diversity was evaluated through their culture characteristics on yeast mannitol agar (YMA) culture media and by polymerase chain reaction Box- (PCR). Two selected isolates were characterized phenotypically regarding their capacity to solubilize phosphate, to grow on media with different concentrations of NaCI, pH's and incubation temperatures. For these the 16S rRNA gene sequencing and symbiotic efficiency using non-autoclaved soils were conducted. The majority of the obtained isolates showed rapid growth and acidified the culture medium. Analysis of the isolates through the Box-PCR revealed low similarity to the reference strain indicated for the culture and a large genetic variability of the obtained isolates. The isolates AM 01 and AM 07, which clustered with Bradyrhizobium and Rhizobium genus, respectively, showed a potential for evaluations and a means to recommend new rhizobia strains for peanut. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-05T11:11:11Z 2014-02-05T11:11:11Z 2014-02-05 2014 2015-03-26T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
African Journal of Microbiology Research, v. 8 n. 6, p. 566-577, fev. 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/978629 |
identifier_str_mv |
African Journal of Microbiology Research, v. 8 n. 6, p. 566-577, fev. 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/978629 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503386443284480 |