Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504 https://doi.org/10.1371/journal.pone.0098758 |
Resumo: | The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations. |
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Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG.DORNELES, E. M. S.SANTANA, J. A.RIBEIRO, D.DORELLA, F. A.GUIMARAES, A. S.MOAWAD, M. S.SELIM, S. A.GARALDI, A. L. M.MIYOSHI, A.RIBEIRO, M. G.GOUVEIA, A. M. G.AZEVEDO, V.HEINEMANN, M. B.LAGE, A. P.2015-04-14T11:11:11Z2015-04-14T11:11:11Z2015-04-1420142015-04-14T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, v. 9, n. 6, e98758, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504https://doi.org/10.1371/journal.pone.0098758enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:13:37Zoai:www.alice.cnptia.embrapa.br:doc/1013504Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:13:37Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
title |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
spellingShingle |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. DORNELES, E. M. S. Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) |
title_short |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
title_full |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
title_fullStr |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
title_full_unstemmed |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
title_sort |
Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates. |
author |
DORNELES, E. M. S. |
author_facet |
DORNELES, E. M. S. SANTANA, J. A. RIBEIRO, D. DORELLA, F. A. GUIMARAES, A. S. MOAWAD, M. S. SELIM, S. A. GARALDI, A. L. M. MIYOSHI, A. RIBEIRO, M. G. GOUVEIA, A. M. G. AZEVEDO, V. HEINEMANN, M. B. LAGE, A. P. |
author_role |
author |
author2 |
SANTANA, J. A. RIBEIRO, D. DORELLA, F. A. GUIMARAES, A. S. MOAWAD, M. S. SELIM, S. A. GARALDI, A. L. M. MIYOSHI, A. RIBEIRO, M. G. GOUVEIA, A. M. G. AZEVEDO, V. HEINEMANN, M. B. LAGE, A. P. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG. |
dc.contributor.author.fl_str_mv |
DORNELES, E. M. S. SANTANA, J. A. RIBEIRO, D. DORELLA, F. A. GUIMARAES, A. S. MOAWAD, M. S. SELIM, S. A. GARALDI, A. L. M. MIYOSHI, A. RIBEIRO, M. G. GOUVEIA, A. M. G. AZEVEDO, V. HEINEMANN, M. B. LAGE, A. P. |
dc.subject.por.fl_str_mv |
Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) |
topic |
Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) |
description |
The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-04-14T11:11:11Z 2015-04-14T11:11:11Z 2015-04-14 2015-04-14T11:11:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, v. 9, n. 6, e98758, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504 https://doi.org/10.1371/journal.pone.0098758 |
identifier_str_mv |
Plos One, v. 9, n. 6, e98758, 2014. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504 https://doi.org/10.1371/journal.pone.0098758 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1822721111670915072 |