Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.

Detalhes bibliográficos
Autor(a) principal: DORNELES, E. M. S.
Data de Publicação: 2014
Outros Autores: SANTANA, J. A., RIBEIRO, D., DORELLA, F. A., GUIMARAES, A. S., MOAWAD, M. S., SELIM, S. A., GARALDI, A. L. M., MIYOSHI, A., RIBEIRO, M. G., GOUVEIA, A. M. G., AZEVEDO, V., HEINEMANN, M. B., LAGE, A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504
https://doi.org/10.1371/journal.pone.0098758
Resumo: The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
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spelling Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG.DORNELES, E. M. S.SANTANA, J. A.RIBEIRO, D.DORELLA, F. A.GUIMARAES, A. S.MOAWAD, M. S.SELIM, S. A.GARALDI, A. L. M.MIYOSHI, A.RIBEIRO, M. G.GOUVEIA, A. M. G.AZEVEDO, V.HEINEMANN, M. B.LAGE, A. P.2015-04-14T11:11:11Z2015-04-14T11:11:11Z2015-04-1420142015-04-14T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, v. 9, n. 6, e98758, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504https://doi.org/10.1371/journal.pone.0098758enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:13:37Zoai:www.alice.cnptia.embrapa.br:doc/1013504Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:13:37Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
title Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
spellingShingle Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
DORNELES, E. M. S.
Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
title_short Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
title_full Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
title_fullStr Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
title_full_unstemmed Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
title_sort Evaluation of ERIC-PCR as Genotyping Method for Corynebacterium pseudotuberculosis Isolates.
author DORNELES, E. M. S.
author_facet DORNELES, E. M. S.
SANTANA, J. A.
RIBEIRO, D.
DORELLA, F. A.
GUIMARAES, A. S.
MOAWAD, M. S.
SELIM, S. A.
GARALDI, A. L. M.
MIYOSHI, A.
RIBEIRO, M. G.
GOUVEIA, A. M. G.
AZEVEDO, V.
HEINEMANN, M. B.
LAGE, A. P.
author_role author
author2 SANTANA, J. A.
RIBEIRO, D.
DORELLA, F. A.
GUIMARAES, A. S.
MOAWAD, M. S.
SELIM, S. A.
GARALDI, A. L. M.
MIYOSHI, A.
RIBEIRO, M. G.
GOUVEIA, A. M. G.
AZEVEDO, V.
HEINEMANN, M. B.
LAGE, A. P.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv ELAINE M. S. DORNELES, UFMG; JORDANA A. SANTANA, UFMG; DAYANA RIBEIRO, UFMG; FERNANDA ALVES DORELLA, UFMG; ALESSANDRO DE SA GUIMARAES, CNPGL; MOHAMED S. MOAWAD, Cairo University, Cairo, Egypt; SALAH A. SELIM, Cairo University, Cairo, Egypt; ANA LUIZA M. GARALDI, UNERJ; ANDERSON MIYOSHI, UFMG; MARCIO G. RIBEIRO, UNESP; AURORA M. G. GOUVEIA, UFMG; VASCO AZEVEDO, UFMG; MARCOS B. HEINEMANN, UFMG; ANDREY P. LAGE, UFMG.
dc.contributor.author.fl_str_mv DORNELES, E. M. S.
SANTANA, J. A.
RIBEIRO, D.
DORELLA, F. A.
GUIMARAES, A. S.
MOAWAD, M. S.
SELIM, S. A.
GARALDI, A. L. M.
MIYOSHI, A.
RIBEIRO, M. G.
GOUVEIA, A. M. G.
AZEVEDO, V.
HEINEMANN, M. B.
LAGE, A. P.
dc.subject.por.fl_str_mv Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
topic Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR)
description The aim of this study was to evaluate the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis isolates from eight different hosts in twelve countries. Ninety-nine C. pseudotuberculosis field strains, one type strain (ATCC 19410T ) and one vaccine strain (1002) were fingerprinted using the ERIC-1R and ERIC-2 primers, and the ERIC-1R+ERIC-2 primer pair. Twenty-nine different genotypes were generated by ERIC 1-PCR, 28 by ERIC 2-PCR and 35 by ERIC 1+2-PCR. The discriminatory index calculated for ERIC 1, ERIC 2, and ERIC 1+2-PCR was 0.89, 0.86, and 0.92, respectively. Epidemiological concordance was established for all ERIC-PCR assays. ERIC 1+2-PCR was defined as the best method based on suitability of the amplification patterns and discriminatory index. Minimal spanning tree for ERIC 1+2-PCR revealed three major clonal complexes and clustering around nitrate-positive (biovar Equi) and nitrate-negative (biovar Ovis) strains. Therefore, ERIC 1+2-PCR proved to be the best technique evaluated in this study for genotyping C. pseudotuberculosis strains, due to its usefulness for molecular epidemiology investigations.
publishDate 2014
dc.date.none.fl_str_mv 2014
2015-04-14T11:11:11Z
2015-04-14T11:11:11Z
2015-04-14
2015-04-14T11:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plos One, v. 9, n. 6, e98758, 2014.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504
https://doi.org/10.1371/journal.pone.0098758
identifier_str_mv Plos One, v. 9, n. 6, e98758, 2014.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1013504
https://doi.org/10.1371/journal.pone.0098758
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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