Optimization of somatic embryogenesis in Coffea canephora.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148351 https://doi.org/10.37118/ijdr.25621.10.2022 |
Resumo: | Plant tissue culture allows the clonal propagation of Coffea canephora on a large scale through somatic embryogenesis. The aim of this study was to optimize the conditions for the induction of somatic embryogenesisin leaves of the Robusta variety. The physiological stage of the leaves - drain or source; the use of the surrounding area ofprimary or secondary veins; and the position of the explants - abaxial or adaxial surface in contact with the culture medium were evaluated. The explants were inoculated in a modified MS culture medium (1/2 macro, 1/4 micronutrients), supplemented with 30 g L-1 sucrose, 6 g L-1 agar, 1.0 mg L-1 2iP and 5.0 mg L-1 IBA. At 45 days of culture, the occurrence of callus induction and the dry weight of the explants were evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The leaf fragments most responsive to the induction of somatic embryogenesis were taken from drain leaves, with explants containing secondary veins, placed with the adaxial face in contact with the culture medium. |
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Optimization of somatic embryogenesis in Coffea canephora.Cotyledonary embryosEmbriogênese somáticaEmbrapa RondôniaPorto Velho (RO)Amazônia OcidentalWestern AmazonCafé RobustaCoffea CanephoraCultura de TecidoCampo ExperimentalTissue cultureSomatic embryogenesisDemonstration farmsPlant tissue culture allows the clonal propagation of Coffea canephora on a large scale through somatic embryogenesis. The aim of this study was to optimize the conditions for the induction of somatic embryogenesisin leaves of the Robusta variety. The physiological stage of the leaves - drain or source; the use of the surrounding area ofprimary or secondary veins; and the position of the explants - abaxial or adaxial surface in contact with the culture medium were evaluated. The explants were inoculated in a modified MS culture medium (1/2 macro, 1/4 micronutrients), supplemented with 30 g L-1 sucrose, 6 g L-1 agar, 1.0 mg L-1 2iP and 5.0 mg L-1 IBA. At 45 days of culture, the occurrence of callus induction and the dry weight of the explants were evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The leaf fragments most responsive to the induction of somatic embryogenesis were taken from drain leaves, with explants containing secondary veins, placed with the adaxial face in contact with the culture medium.CAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.SILVA, C. K. C. daSANTOS, M. R. A. dos2022-11-16T20:01:57Z2022-11-16T20:01:57Z2022-11-162022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInternational Journal of Development Research, v. 12, n. 10, p. 59738-59740, Oct. 2022.2230-9926http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148351https://doi.org/10.37118/ijdr.25621.10.2022enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2022-11-16T20:01:57Zoai:www.alice.cnptia.embrapa.br:doc/1148351Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542022-11-16T20:01:57falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542022-11-16T20:01:57Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Optimization of somatic embryogenesis in Coffea canephora. |
title |
Optimization of somatic embryogenesis in Coffea canephora. |
spellingShingle |
Optimization of somatic embryogenesis in Coffea canephora. SILVA, C. K. C. da Cotyledonary embryos Embriogênese somática Embrapa Rondônia Porto Velho (RO) Amazônia Ocidental Western Amazon Café Robusta Coffea Canephora Cultura de Tecido Campo Experimental Tissue culture Somatic embryogenesis Demonstration farms |
title_short |
Optimization of somatic embryogenesis in Coffea canephora. |
title_full |
Optimization of somatic embryogenesis in Coffea canephora. |
title_fullStr |
Optimization of somatic embryogenesis in Coffea canephora. |
title_full_unstemmed |
Optimization of somatic embryogenesis in Coffea canephora. |
title_sort |
Optimization of somatic embryogenesis in Coffea canephora. |
author |
SILVA, C. K. C. da |
author_facet |
SILVA, C. K. C. da SANTOS, M. R. A. dos |
author_role |
author |
author2 |
SANTOS, M. R. A. dos |
author2_role |
author |
dc.contributor.none.fl_str_mv |
CAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO. |
dc.contributor.author.fl_str_mv |
SILVA, C. K. C. da SANTOS, M. R. A. dos |
dc.subject.por.fl_str_mv |
Cotyledonary embryos Embriogênese somática Embrapa Rondônia Porto Velho (RO) Amazônia Ocidental Western Amazon Café Robusta Coffea Canephora Cultura de Tecido Campo Experimental Tissue culture Somatic embryogenesis Demonstration farms |
topic |
Cotyledonary embryos Embriogênese somática Embrapa Rondônia Porto Velho (RO) Amazônia Ocidental Western Amazon Café Robusta Coffea Canephora Cultura de Tecido Campo Experimental Tissue culture Somatic embryogenesis Demonstration farms |
description |
Plant tissue culture allows the clonal propagation of Coffea canephora on a large scale through somatic embryogenesis. The aim of this study was to optimize the conditions for the induction of somatic embryogenesisin leaves of the Robusta variety. The physiological stage of the leaves - drain or source; the use of the surrounding area ofprimary or secondary veins; and the position of the explants - abaxial or adaxial surface in contact with the culture medium were evaluated. The explants were inoculated in a modified MS culture medium (1/2 macro, 1/4 micronutrients), supplemented with 30 g L-1 sucrose, 6 g L-1 agar, 1.0 mg L-1 2iP and 5.0 mg L-1 IBA. At 45 days of culture, the occurrence of callus induction and the dry weight of the explants were evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The leaf fragments most responsive to the induction of somatic embryogenesis were taken from drain leaves, with explants containing secondary veins, placed with the adaxial face in contact with the culture medium. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-16T20:01:57Z 2022-11-16T20:01:57Z 2022-11-16 2022 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal of Development Research, v. 12, n. 10, p. 59738-59740, Oct. 2022. 2230-9926 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148351 https://doi.org/10.37118/ijdr.25621.10.2022 |
identifier_str_mv |
International Journal of Development Research, v. 12, n. 10, p. 59738-59740, Oct. 2022. 2230-9926 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148351 https://doi.org/10.37118/ijdr.25621.10.2022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503534404698112 |