Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153370 https://doi.org/10.1111/jbg.12777 |
Resumo: | Individual variation in milk fatty acid (FA) composition has been partially attrib-uted to stearoyl- CoA desaturase 1 (SCD1) gene polymorphisms in taurine breeds, but much less is known for Zebu breeds. This study investigated the phenotypic variation in milk FA composition, and the influence of SCD1 variants on this trait and on milk fat desaturase indices (DI) in Gir cows. The functional impact of SCD1 variants was predicted using bioinformatics tools. Milk and blood samples were collected from 312 cows distributed in 10 herds from five states of Brazil. SCD1 variants were identified through target sequencing, and milk FA composi-tion was determined by gas chromatography. Phenotypic variation in milk FA composition fell within the range reported for taurine breeds, with SCD18 index showing the lowest variation among the DI. Fourteen SCD1 variants were identi-fied, six of which not previously described. Regarding the A293V polymorphism, all cows were homozygous for the C allele (coding for alanine), whereas all gen-otypes were detected for the second SNP affecting the 293 codon (G > A), with compelling evidence for functional effects. Significant associations (based on raw p- values) were found between this SNP and C12:0, cis- 9, trans- 11 CLA and short- chain FA, and between another SNP (rs523411937) and C15:0 and odd- chain lin-ear FA. A new SNP on Chr26:21277069 was associated with trans- 11 C18:1, cis- 9, trans- 11 CLA, C18:3 n- 3 and n- 3 FA. These findings indicate that SCD1 polymor-phisms also contributes to the phenotypic variation in milk FA composition of Gir cows, with potential use in their breeding programmes. |
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Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows.Enzimas lipogênicasGado LeiteiroProdução LeiteiraÁcido GraxoGado GirGordura LácteaSaúdeHuman healthBreedsZebu breedsIndividual variation in milk fatty acid (FA) composition has been partially attrib-uted to stearoyl- CoA desaturase 1 (SCD1) gene polymorphisms in taurine breeds, but much less is known for Zebu breeds. This study investigated the phenotypic variation in milk FA composition, and the influence of SCD1 variants on this trait and on milk fat desaturase indices (DI) in Gir cows. The functional impact of SCD1 variants was predicted using bioinformatics tools. Milk and blood samples were collected from 312 cows distributed in 10 herds from five states of Brazil. SCD1 variants were identified through target sequencing, and milk FA composi-tion was determined by gas chromatography. Phenotypic variation in milk FA composition fell within the range reported for taurine breeds, with SCD18 index showing the lowest variation among the DI. Fourteen SCD1 variants were identi-fied, six of which not previously described. Regarding the A293V polymorphism, all cows were homozygous for the C allele (coding for alanine), whereas all gen-otypes were detected for the second SNP affecting the 293 codon (G > A), with compelling evidence for functional effects. Significant associations (based on raw p- values) were found between this SNP and C12:0, cis- 9, trans- 11 CLA and short- chain FA, and between another SNP (rs523411937) and C15:0 and odd- chain lin-ear FA. A new SNP on Chr26:21277069 was associated with trans- 11 C18:1, cis- 9, trans- 11 CLA, C18:3 n- 3 and n- 3 FA. These findings indicate that SCD1 polymor-phisms also contributes to the phenotypic variation in milk FA composition of Gir cows, with potential use in their breeding programmes.CAROLINA GUIMARÃES RAMOS MATOSINHO, Universidade Federal de Minas Gerais; PABLO AUGUSTO DE SOUZA FONSECA, University of Guelph; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; IZINARA CRUZ ROSSE, Universidade Federal de Ouro Preto; FERNANDO CESAR FERRAZ LOPES, CNPGL; THALIA ZÓZIMO, Universidade Federal de Minas Gerais; ANÍBAL EUGÊNIO VERCESI FILHO, Instituto de Zootecnia do Governo do Estado de São Paulo; FRANK ANGELO TOMITA BRUNELI, CNPGL; MARIA RAQUEL SANTOS CARVALHO, Universidade Federal de Minas Gerais; MARCO ANTONIO SUNDFELD DA GAMA, CPPSE.MATOSINHO, C. G. R.FONSECA, P. A. de S.PEIXOTO, M. G. C. D.ROSSE, I. C.LOPES, F. C. F.ZÓZIMO, T.VERCESI FILHO, A. E.BRUNELI, F. A. T.CARVALHO, M. R. S.GAMA, M. A. S. da2023-09-12T10:28:03Z2023-09-12T10:28:03Z2023-04-262023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Animal Breeding and Genetics, v. 140, n. 5, p. 532-548, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153370https://doi.org/10.1111/jbg.12777enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-12T10:28:03Zoai:www.alice.cnptia.embrapa.br:doc/1153370Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-12T10:28:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-12T10:28:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows. |
title |
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows. |
spellingShingle |
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows. MATOSINHO, C. G. R. Enzimas lipogênicas Gado Leiteiro Produção Leiteira Ácido Graxo Gado Gir Gordura Láctea Saúde Human health Breeds Zebu breeds |
title_short |
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows. |
title_full |
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows. |
title_fullStr |
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows. |
title_full_unstemmed |
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows. |
title_sort |
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows. |
author |
MATOSINHO, C. G. R. |
author_facet |
MATOSINHO, C. G. R. FONSECA, P. A. de S. PEIXOTO, M. G. C. D. ROSSE, I. C. LOPES, F. C. F. ZÓZIMO, T. VERCESI FILHO, A. E. BRUNELI, F. A. T. CARVALHO, M. R. S. GAMA, M. A. S. da |
author_role |
author |
author2 |
FONSECA, P. A. de S. PEIXOTO, M. G. C. D. ROSSE, I. C. LOPES, F. C. F. ZÓZIMO, T. VERCESI FILHO, A. E. BRUNELI, F. A. T. CARVALHO, M. R. S. GAMA, M. A. S. da |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
CAROLINA GUIMARÃES RAMOS MATOSINHO, Universidade Federal de Minas Gerais; PABLO AUGUSTO DE SOUZA FONSECA, University of Guelph; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; IZINARA CRUZ ROSSE, Universidade Federal de Ouro Preto; FERNANDO CESAR FERRAZ LOPES, CNPGL; THALIA ZÓZIMO, Universidade Federal de Minas Gerais; ANÍBAL EUGÊNIO VERCESI FILHO, Instituto de Zootecnia do Governo do Estado de São Paulo; FRANK ANGELO TOMITA BRUNELI, CNPGL; MARIA RAQUEL SANTOS CARVALHO, Universidade Federal de Minas Gerais; MARCO ANTONIO SUNDFELD DA GAMA, CPPSE. |
dc.contributor.author.fl_str_mv |
MATOSINHO, C. G. R. FONSECA, P. A. de S. PEIXOTO, M. G. C. D. ROSSE, I. C. LOPES, F. C. F. ZÓZIMO, T. VERCESI FILHO, A. E. BRUNELI, F. A. T. CARVALHO, M. R. S. GAMA, M. A. S. da |
dc.subject.por.fl_str_mv |
Enzimas lipogênicas Gado Leiteiro Produção Leiteira Ácido Graxo Gado Gir Gordura Láctea Saúde Human health Breeds Zebu breeds |
topic |
Enzimas lipogênicas Gado Leiteiro Produção Leiteira Ácido Graxo Gado Gir Gordura Láctea Saúde Human health Breeds Zebu breeds |
description |
Individual variation in milk fatty acid (FA) composition has been partially attrib-uted to stearoyl- CoA desaturase 1 (SCD1) gene polymorphisms in taurine breeds, but much less is known for Zebu breeds. This study investigated the phenotypic variation in milk FA composition, and the influence of SCD1 variants on this trait and on milk fat desaturase indices (DI) in Gir cows. The functional impact of SCD1 variants was predicted using bioinformatics tools. Milk and blood samples were collected from 312 cows distributed in 10 herds from five states of Brazil. SCD1 variants were identified through target sequencing, and milk FA composi-tion was determined by gas chromatography. Phenotypic variation in milk FA composition fell within the range reported for taurine breeds, with SCD18 index showing the lowest variation among the DI. Fourteen SCD1 variants were identi-fied, six of which not previously described. Regarding the A293V polymorphism, all cows were homozygous for the C allele (coding for alanine), whereas all gen-otypes were detected for the second SNP affecting the 293 codon (G > A), with compelling evidence for functional effects. Significant associations (based on raw p- values) were found between this SNP and C12:0, cis- 9, trans- 11 CLA and short- chain FA, and between another SNP (rs523411937) and C15:0 and odd- chain lin-ear FA. A new SNP on Chr26:21277069 was associated with trans- 11 C18:1, cis- 9, trans- 11 CLA, C18:3 n- 3 and n- 3 FA. These findings indicate that SCD1 polymor-phisms also contributes to the phenotypic variation in milk FA composition of Gir cows, with potential use in their breeding programmes. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-12T10:28:03Z 2023-09-12T10:28:03Z 2023-04-26 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Animal Breeding and Genetics, v. 140, n. 5, p. 532-548, 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153370 https://doi.org/10.1111/jbg.12777 |
identifier_str_mv |
Journal of Animal Breeding and Genetics, v. 140, n. 5, p. 532-548, 2023. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153370 https://doi.org/10.1111/jbg.12777 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503549172842496 |