Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133043 https://doi.org/10.1038/s41598-021-86689-y |
Resumo: | The aim of this study was to identify the correlation between photochemical efficiency and candidate genes expression to elucidate the drought tolerance mechanisms in coffee progenies (Icatu Vermelho IAC 3851-2 x Catimor UFV 1602-215) previously identified as tolerant in field conditions. Four progenies (2, 5, 12 and 15) were evaluated under water-deficit conditions (water deficit imposed 8 months after transplanting seedlings to the pots) and under irrigated system. Evaluations of physiological parameters and expression of candidate genes for drought tolerance were performed. Progeny 5 showed capacity to maintain water potential, which contributed to lower qP variation between irrigated and deficit conditions. However, the increases of qN and NPQ in response to stress indicate that this progeny is photochemically responsive to small variations of am protecting the photosystem and maintaining qP. Data obtained for progeny 12 indicated a lower water status maintenance capacity, but with increased qN and NPQ providing maintenance of the PSII and ETR parameters. A PCA analysis revealed that the genes coding regulatory proteins, ABA-synthesis, cellular protectors, isoforms of ascorbate peroxidase clearly displayed a major response to drought stress and discriminated the progenies 5 and 12 which showed a better photochemical response. The genes CaMYB1, CaERF017, CaEDR2, CaNCED, CaAPX1, CaAPX5, CaGolS3, CaDHN1 and CaPYL8a were up-regulated in the arabica coffee progenies with greater photochemical efficiency under deficit and therefore contributing to efficiency of the photosynthesis in drought tolerant progenies. |
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Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance.Resistência a SecaResistência GenéticaCoffea ArábicaPhotochemical reactionsDrought toleranceCoffea arabica var. arabicaThe aim of this study was to identify the correlation between photochemical efficiency and candidate genes expression to elucidate the drought tolerance mechanisms in coffee progenies (Icatu Vermelho IAC 3851-2 x Catimor UFV 1602-215) previously identified as tolerant in field conditions. Four progenies (2, 5, 12 and 15) were evaluated under water-deficit conditions (water deficit imposed 8 months after transplanting seedlings to the pots) and under irrigated system. Evaluations of physiological parameters and expression of candidate genes for drought tolerance were performed. Progeny 5 showed capacity to maintain water potential, which contributed to lower qP variation between irrigated and deficit conditions. However, the increases of qN and NPQ in response to stress indicate that this progeny is photochemically responsive to small variations of am protecting the photosystem and maintaining qP. Data obtained for progeny 12 indicated a lower water status maintenance capacity, but with increased qN and NPQ providing maintenance of the PSII and ETR parameters. A PCA analysis revealed that the genes coding regulatory proteins, ABA-synthesis, cellular protectors, isoforms of ascorbate peroxidase clearly displayed a major response to drought stress and discriminated the progenies 5 and 12 which showed a better photochemical response. The genes CaMYB1, CaERF017, CaEDR2, CaNCED, CaAPX1, CaAPX5, CaGolS3, CaDHN1 and CaPYL8a were up-regulated in the arabica coffee progenies with greater photochemical efficiency under deficit and therefore contributing to efficiency of the photosynthesis in drought tolerant progenies.MELINE DE OLIVEIRA SANTOS, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS; LARISSA SOUSA COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; GLADYSTON RODRIGUES CARVALHO, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS; CESAR ELIAS BOTELHO, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS; LUANA FERREIRA TORRES, UNIVERSIDADE FEDERAL DE LAVRAS; DIEGO JÚNIOR MARTINS VILELA, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS; ALAN CARVALHO ANDRADE, CNPCa; VÂNIA APARECIDA SILVA, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS.SANTOS, M. de O.COELHO, L. S.CARVALHO, G. R.BOTELHO, C. E.TORRES, L. F.VILELA, D. J. M.ANDRADE, A. C.SILVA, V. A.2021-07-20T15:01:13Z2021-07-20T15:01:13Z2021-07-202021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14 p.Scientific Reports, v. 11, 7436, 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133043https://doi.org/10.1038/s41598-021-86689-yenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-07-20T15:01:22Zoai:www.alice.cnptia.embrapa.br:doc/1133043Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-07-20T15:01:22falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-07-20T15:01:22Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. |
title |
Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. |
spellingShingle |
Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. SANTOS, M. de O. Resistência a Seca Resistência Genética Coffea Arábica Photochemical reactions Drought tolerance Coffea arabica var. arabica |
title_short |
Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. |
title_full |
Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. |
title_fullStr |
Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. |
title_full_unstemmed |
Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. |
title_sort |
Photochemical efficiency correlated with candidate gene expression promote coffee drought tolerance. |
author |
SANTOS, M. de O. |
author_facet |
SANTOS, M. de O. COELHO, L. S. CARVALHO, G. R. BOTELHO, C. E. TORRES, L. F. VILELA, D. J. M. ANDRADE, A. C. SILVA, V. A. |
author_role |
author |
author2 |
COELHO, L. S. CARVALHO, G. R. BOTELHO, C. E. TORRES, L. F. VILELA, D. J. M. ANDRADE, A. C. SILVA, V. A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
MELINE DE OLIVEIRA SANTOS, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS; LARISSA SOUSA COELHO, UNIVERSIDADE FEDERAL DE LAVRAS; GLADYSTON RODRIGUES CARVALHO, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS; CESAR ELIAS BOTELHO, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS; LUANA FERREIRA TORRES, UNIVERSIDADE FEDERAL DE LAVRAS; DIEGO JÚNIOR MARTINS VILELA, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS; ALAN CARVALHO ANDRADE, CNPCa; VÂNIA APARECIDA SILVA, EMPRESA DE PESQUISA AGROPECUÁRIA DE MINAS GERAIS. |
dc.contributor.author.fl_str_mv |
SANTOS, M. de O. COELHO, L. S. CARVALHO, G. R. BOTELHO, C. E. TORRES, L. F. VILELA, D. J. M. ANDRADE, A. C. SILVA, V. A. |
dc.subject.por.fl_str_mv |
Resistência a Seca Resistência Genética Coffea Arábica Photochemical reactions Drought tolerance Coffea arabica var. arabica |
topic |
Resistência a Seca Resistência Genética Coffea Arábica Photochemical reactions Drought tolerance Coffea arabica var. arabica |
description |
The aim of this study was to identify the correlation between photochemical efficiency and candidate genes expression to elucidate the drought tolerance mechanisms in coffee progenies (Icatu Vermelho IAC 3851-2 x Catimor UFV 1602-215) previously identified as tolerant in field conditions. Four progenies (2, 5, 12 and 15) were evaluated under water-deficit conditions (water deficit imposed 8 months after transplanting seedlings to the pots) and under irrigated system. Evaluations of physiological parameters and expression of candidate genes for drought tolerance were performed. Progeny 5 showed capacity to maintain water potential, which contributed to lower qP variation between irrigated and deficit conditions. However, the increases of qN and NPQ in response to stress indicate that this progeny is photochemically responsive to small variations of am protecting the photosystem and maintaining qP. Data obtained for progeny 12 indicated a lower water status maintenance capacity, but with increased qN and NPQ providing maintenance of the PSII and ETR parameters. A PCA analysis revealed that the genes coding regulatory proteins, ABA-synthesis, cellular protectors, isoforms of ascorbate peroxidase clearly displayed a major response to drought stress and discriminated the progenies 5 and 12 which showed a better photochemical response. The genes CaMYB1, CaERF017, CaEDR2, CaNCED, CaAPX1, CaAPX5, CaGolS3, CaDHN1 and CaPYL8a were up-regulated in the arabica coffee progenies with greater photochemical efficiency under deficit and therefore contributing to efficiency of the photosynthesis in drought tolerant progenies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-20T15:01:13Z 2021-07-20T15:01:13Z 2021-07-20 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Scientific Reports, v. 11, 7436, 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133043 https://doi.org/10.1038/s41598-021-86689-y |
identifier_str_mv |
Scientific Reports, v. 11, 7436, 2021. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133043 https://doi.org/10.1038/s41598-021-86689-y |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 p. |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503507074613248 |