Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers.
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122034 https://doi.org/10.1590/S1415-47572003000400008 |
Resumo: | The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, RST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure. |
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Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers.Genetic diversityTropical treeSSREugenia DysentericaCagaitaCerradoVariação GenéticaMarcador MolecularMyrtaceaeThe ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, RST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure.MARIA IMACULADA ZUCCHI, ESALQ; ROSANA PEREIRA VIANELLO, CNPAF; JOSE BALDIN PINHEIRO, UFG; LAZARO JOSE CHAVES, UFG; ALEXANDRE SIQUEIRA GUEDES COELHO, UFG; ROLAND VENCOVSKY, ESALQ.ZUCCHI, M. I.BRONDANI, R. P. V.PINHEIRO, J. B.CHAVES, L. J.COELHO, A. S. G.VENCOVSKY, R.2020-05-05T04:39:47Z2020-05-05T04:39:47Z2020-05-042003info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biology, v. 26, n. 4, p. 449-457, dez. 2003.1678-4685http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122034https://doi.org/10.1590/S1415-47572003000400008enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-05-05T04:39:54Zoai:www.alice.cnptia.embrapa.br:doc/1122034Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-05-05T04:39:54Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. |
title |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. |
spellingShingle |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. ZUCCHI, M. I. Genetic diversity Tropical tree SSR Eugenia Dysenterica Cagaita Cerrado Variação Genética Marcador Molecular Myrtaceae ZUCCHI, M. I. Genetic diversity Tropical tree SSR Eugenia Dysenterica Cagaita Cerrado Variação Genética Marcador Molecular Myrtaceae |
title_short |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. |
title_full |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. |
title_fullStr |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. |
title_full_unstemmed |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. |
title_sort |
Genetic structure and gene flow in Eugenia dysenterica DC in the Brazilian Cerrado utilizing SSR markers. |
author |
ZUCCHI, M. I. |
author_facet |
ZUCCHI, M. I. ZUCCHI, M. I. BRONDANI, R. P. V. PINHEIRO, J. B. CHAVES, L. J. COELHO, A. S. G. VENCOVSKY, R. BRONDANI, R. P. V. PINHEIRO, J. B. CHAVES, L. J. COELHO, A. S. G. VENCOVSKY, R. |
author_role |
author |
author2 |
BRONDANI, R. P. V. PINHEIRO, J. B. CHAVES, L. J. COELHO, A. S. G. VENCOVSKY, R. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
MARIA IMACULADA ZUCCHI, ESALQ; ROSANA PEREIRA VIANELLO, CNPAF; JOSE BALDIN PINHEIRO, UFG; LAZARO JOSE CHAVES, UFG; ALEXANDRE SIQUEIRA GUEDES COELHO, UFG; ROLAND VENCOVSKY, ESALQ. |
dc.contributor.author.fl_str_mv |
ZUCCHI, M. I. BRONDANI, R. P. V. PINHEIRO, J. B. CHAVES, L. J. COELHO, A. S. G. VENCOVSKY, R. |
dc.subject.por.fl_str_mv |
Genetic diversity Tropical tree SSR Eugenia Dysenterica Cagaita Cerrado Variação Genética Marcador Molecular Myrtaceae |
topic |
Genetic diversity Tropical tree SSR Eugenia Dysenterica Cagaita Cerrado Variação Genética Marcador Molecular Myrtaceae |
description |
The ''cagaita tree'' (Eugenia dysenterica) is a plant found widespread in the Brazilian Cerrado. Its fruit is used for popular consumption and for industrial purposes. This study opens a new perspective for the generation of population genetic data and parameters estimates for devising sound collection and conservation procedures for Eugenia dysenterica. A battery of 356 primer pairs developed for Eucalyptus spp. was tested on the ''cagaita tree''. Only 10 primer pairs were found to be transferable between the two species. Using a polyacrilamide gel, an average of 10.4 alleles per locus was detected, in a sample of 116 individuals from 10 natural ''cagaita tree'' populations. Seven polymorphic loci allowed estimation of genetic parameters, including expected average heterozygosity He = 0,442, among population diversity, RST = 0,268 and gene flow Nm = 0,680. Results indicated a potential of SSR locus transferability developed for Eucalyptus to other species of different genera, such as in the case of the ''cagaita tree''. The high genetic diversity among populations detected with SSR markers indicated that these markers are highly sensitive to detect population structure. Estimated Nm values and the existence of private alleles indicated reduced gene flow and consequently possible damage to the metapopulation structure. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003 2020-05-05T04:39:47Z 2020-05-05T04:39:47Z 2020-05-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Biology, v. 26, n. 4, p. 449-457, dez. 2003. 1678-4685 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122034 https://doi.org/10.1590/S1415-47572003000400008 |
identifier_str_mv |
Genetics and Molecular Biology, v. 26, n. 4, p. 449-457, dez. 2003. 1678-4685 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122034 https://doi.org/10.1590/S1415-47572003000400008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1822180768451919872 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1415-47572003000400008 |