Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153549 |
Resumo: | Differential rootstock tolerance to Fusarium spp. supports viticulture worldwide. However, how plants stand against the fungus still needs to be explored. We hypothesize it involves a differential metabolite modulation. Thus, we performed a gas chromatography coupled with mass spectrometry (GC?MS) analysis of Paulsen P1103 and BDMG573 rootstocks, co-cultured with Fusarium oxysporum (FUS) for short, medium, and long time (0, 4, and 8 days after treatment [DAT]). In shoots, principal component analysis (PCA) showed a complete overlap between BDMG573 non-cocultivated and FUS at 0 DAT, and P1103 treatments showed a slight overlap at both 4 and 8 DAT. In roots, PCA exhibited overlapping between BDMG573 treatments at 0 DAT, while P1103 treatments showed overlapping at 0 and 4 DAT. Further, there is a complete overlapping between BDMG573 and P1103 FUS profiles at 8 DAT. In shoots, 1,3-dihydroxyacetone at 0 and 4 DAT and maltose at 4 and 8 DAT were biomarkers for BDMG573. For P1103, glyceric acid, proline, and sorbitol stood out at 0, 4, and 8 DAT, respectively. In BDMG573 roots, the biomarkers were β-alanine at 0 DAT, cellobiose and sorbitol at both 4 and 8 DAT. While in P1103 roots, they were galactose at 0 and 4 DAT and 1,3-dihydroxyacetone at 8 DAT. Overall, there is an increase in amino acids, glycolysis, and tricarboxylic acid components in tolerant Paulsen P1103 shoots. Thus, it provides a new perspective on the primary metabolism of grapevine rootstocks to F. oxysporum that may contribute to strategies for genotype tolerance and early disease identification. |
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Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times.Paulsen P1103Paulsen BDMG573Fusarium sppFusariumFusarium OxysporumDifferential rootstock tolerance to Fusarium spp. supports viticulture worldwide. However, how plants stand against the fungus still needs to be explored. We hypothesize it involves a differential metabolite modulation. Thus, we performed a gas chromatography coupled with mass spectrometry (GC?MS) analysis of Paulsen P1103 and BDMG573 rootstocks, co-cultured with Fusarium oxysporum (FUS) for short, medium, and long time (0, 4, and 8 days after treatment [DAT]). In shoots, principal component analysis (PCA) showed a complete overlap between BDMG573 non-cocultivated and FUS at 0 DAT, and P1103 treatments showed a slight overlap at both 4 and 8 DAT. In roots, PCA exhibited overlapping between BDMG573 treatments at 0 DAT, while P1103 treatments showed overlapping at 0 and 4 DAT. Further, there is a complete overlapping between BDMG573 and P1103 FUS profiles at 8 DAT. In shoots, 1,3-dihydroxyacetone at 0 and 4 DAT and maltose at 4 and 8 DAT were biomarkers for BDMG573. For P1103, glyceric acid, proline, and sorbitol stood out at 0, 4, and 8 DAT, respectively. In BDMG573 roots, the biomarkers were β-alanine at 0 DAT, cellobiose and sorbitol at both 4 and 8 DAT. While in P1103 roots, they were galactose at 0 and 4 DAT and 1,3-dihydroxyacetone at 8 DAT. Overall, there is an increase in amino acids, glycolysis, and tricarboxylic acid components in tolerant Paulsen P1103 shoots. Thus, it provides a new perspective on the primary metabolism of grapevine rootstocks to F. oxysporum that may contribute to strategies for genotype tolerance and early disease identification.IGOR RAFAEL SOUSA COSTA, FEDERAL UNIVERSITY OF CEARÁ; FRANCISCO LUCAS PACHECO CAVALCANTE, FEDERAL UNIVERSITY OF CEARÁ; FABIO ROSSI CAVALCANTI, CNPUV; CLEBERSON DE FREITAS FERNANDES, CNPAT; ENÉAS GOMES-FILHO, FEDERAL UNIVERSITY OF CEARÁ; KIRLEY MARQUES CANUTO, CNPAT; HUMBERTO HENRIQUE DE CARVALHO, FEDERAL UNIVERSITY OF CEARÁ.COSTA, I. R. S. C.CAVALCANTE, F. L. P.CAVALCANTI, F. R.FERNANDES, C. de F.GOMES-FILHO, E.CANUTO, K. M.CARVALHO, H. H. DE2023-05-08T13:51:38Z2023-05-08T13:51:38Z2023-05-082023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePhysiologia Plantarum, v. 175, e13918, April 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/115354910.1111/ppl.13918enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-05-08T13:51:38Zoai:www.alice.cnptia.embrapa.br:doc/1153549Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-05-08T13:51:38falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-05-08T13:51:38Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times. |
title |
Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times. |
spellingShingle |
Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times. COSTA, I. R. S. C. Paulsen P1103 Paulsen BDMG573 Fusarium spp Fusarium Fusarium Oxysporum |
title_short |
Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times. |
title_full |
Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times. |
title_fullStr |
Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times. |
title_full_unstemmed |
Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times. |
title_sort |
Metabolomic exhibits different profiles and potential biomarkers of Vitis spp. co-cultivated with Fusarium oxysporum for short, medium, and long times. |
author |
COSTA, I. R. S. C. |
author_facet |
COSTA, I. R. S. C. CAVALCANTE, F. L. P. CAVALCANTI, F. R. FERNANDES, C. de F. GOMES-FILHO, E. CANUTO, K. M. CARVALHO, H. H. DE |
author_role |
author |
author2 |
CAVALCANTE, F. L. P. CAVALCANTI, F. R. FERNANDES, C. de F. GOMES-FILHO, E. CANUTO, K. M. CARVALHO, H. H. DE |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
IGOR RAFAEL SOUSA COSTA, FEDERAL UNIVERSITY OF CEARÁ; FRANCISCO LUCAS PACHECO CAVALCANTE, FEDERAL UNIVERSITY OF CEARÁ; FABIO ROSSI CAVALCANTI, CNPUV; CLEBERSON DE FREITAS FERNANDES, CNPAT; ENÉAS GOMES-FILHO, FEDERAL UNIVERSITY OF CEARÁ; KIRLEY MARQUES CANUTO, CNPAT; HUMBERTO HENRIQUE DE CARVALHO, FEDERAL UNIVERSITY OF CEARÁ. |
dc.contributor.author.fl_str_mv |
COSTA, I. R. S. C. CAVALCANTE, F. L. P. CAVALCANTI, F. R. FERNANDES, C. de F. GOMES-FILHO, E. CANUTO, K. M. CARVALHO, H. H. DE |
dc.subject.por.fl_str_mv |
Paulsen P1103 Paulsen BDMG573 Fusarium spp Fusarium Fusarium Oxysporum |
topic |
Paulsen P1103 Paulsen BDMG573 Fusarium spp Fusarium Fusarium Oxysporum |
description |
Differential rootstock tolerance to Fusarium spp. supports viticulture worldwide. However, how plants stand against the fungus still needs to be explored. We hypothesize it involves a differential metabolite modulation. Thus, we performed a gas chromatography coupled with mass spectrometry (GC?MS) analysis of Paulsen P1103 and BDMG573 rootstocks, co-cultured with Fusarium oxysporum (FUS) for short, medium, and long time (0, 4, and 8 days after treatment [DAT]). In shoots, principal component analysis (PCA) showed a complete overlap between BDMG573 non-cocultivated and FUS at 0 DAT, and P1103 treatments showed a slight overlap at both 4 and 8 DAT. In roots, PCA exhibited overlapping between BDMG573 treatments at 0 DAT, while P1103 treatments showed overlapping at 0 and 4 DAT. Further, there is a complete overlapping between BDMG573 and P1103 FUS profiles at 8 DAT. In shoots, 1,3-dihydroxyacetone at 0 and 4 DAT and maltose at 4 and 8 DAT were biomarkers for BDMG573. For P1103, glyceric acid, proline, and sorbitol stood out at 0, 4, and 8 DAT, respectively. In BDMG573 roots, the biomarkers were β-alanine at 0 DAT, cellobiose and sorbitol at both 4 and 8 DAT. While in P1103 roots, they were galactose at 0 and 4 DAT and 1,3-dihydroxyacetone at 8 DAT. Overall, there is an increase in amino acids, glycolysis, and tricarboxylic acid components in tolerant Paulsen P1103 shoots. Thus, it provides a new perspective on the primary metabolism of grapevine rootstocks to F. oxysporum that may contribute to strategies for genotype tolerance and early disease identification. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-08T13:51:38Z 2023-05-08T13:51:38Z 2023-05-08 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Physiologia Plantarum, v. 175, e13918, April 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153549 10.1111/ppl.13918 |
identifier_str_mv |
Physiologia Plantarum, v. 175, e13918, April 2023. 10.1111/ppl.13918 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1153549 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503544565399552 |