Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant.

• Autor(a) principal: ARAÚJO, M. da C. da R.
• Data de Publicação: 2021
• Outros Autores: CHAGAS, E. A., VENDRAME, W., RIBEIRO, M. I. G., MOURA, E. A. de, TAVEIRA, D. L. L., CHAGAS, P. C., GRIGIO, M. L.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1139460
• topic: Camu-camu; Somatic embryogenesis; Growth regulators

Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.