Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065905 |
Resumo: | Essential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut. |
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Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut.Ocimum Basicilum LAranha ácaroSpider mitesAmendoimTetranychus urticaeCromatografiaPeanutsChromatographyEugenolEssential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut.MARIA ISABEL G. MARTINS, RENORBIO/UFRPE; ANTONIO EUZÉBIO G. SANT'ANA, UFAL; FELIPE MATHEUS T. VASCONCELOS, UFRPE; WBYRATAN L. SILVA, UFAL; LIZIANE MARIA DE LIMA, CNPA; REGINALDO CARVALHO, UFRPE; PÉRICLES A. MELO FILHO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA.MARTINS, M. I. G.SANT'ANA, A. E. G.VASCONCELOS, F. M. T.SILVA, W. L.LIMA, L. M. deCARVALHO, R.MELO FILHO, P. A.SANTOS, R. C. dos2017-03-03T23:57:51Z2017-03-03T23:57:51Z2017-03-0320162017-03-03T23:57:51Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAfrican Journal of Biotechnology, v. 15, n. 30, p. 1597-1607, July 2016.1684-5315http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065905enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:16:01Zoai:www.alice.cnptia.embrapa.br:doc/1065905Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:16:01falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:16:01Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
title |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
spellingShingle |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. MARTINS, M. I. G. Ocimum Basicilum L Aranha ácaro Spider mites Amendoim Tetranychus urticae Cromatografia Peanuts Chromatography Eugenol |
title_short |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
title_full |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
title_fullStr |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
title_full_unstemmed |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
title_sort |
Bioactivity of basil (Ocimum basicilum L.) on control of the spider mite (Tetranychus urticae Koch.) in peanut. |
author |
MARTINS, M. I. G. |
author_facet |
MARTINS, M. I. G. SANT'ANA, A. E. G. VASCONCELOS, F. M. T. SILVA, W. L. LIMA, L. M. de CARVALHO, R. MELO FILHO, P. A. SANTOS, R. C. dos |
author_role |
author |
author2 |
SANT'ANA, A. E. G. VASCONCELOS, F. M. T. SILVA, W. L. LIMA, L. M. de CARVALHO, R. MELO FILHO, P. A. SANTOS, R. C. dos |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
MARIA ISABEL G. MARTINS, RENORBIO/UFRPE; ANTONIO EUZÉBIO G. SANT'ANA, UFAL; FELIPE MATHEUS T. VASCONCELOS, UFRPE; WBYRATAN L. SILVA, UFAL; LIZIANE MARIA DE LIMA, CNPA; REGINALDO CARVALHO, UFRPE; PÉRICLES A. MELO FILHO, UFRPE; ROSEANE CAVALCANTI DOS SANTOS, CNPA. |
dc.contributor.author.fl_str_mv |
MARTINS, M. I. G. SANT'ANA, A. E. G. VASCONCELOS, F. M. T. SILVA, W. L. LIMA, L. M. de CARVALHO, R. MELO FILHO, P. A. SANTOS, R. C. dos |
dc.subject.por.fl_str_mv |
Ocimum Basicilum L Aranha ácaro Spider mites Amendoim Tetranychus urticae Cromatografia Peanuts Chromatography Eugenol |
topic |
Ocimum Basicilum L Aranha ácaro Spider mites Amendoim Tetranychus urticae Cromatografia Peanuts Chromatography Eugenol |
description |
Essential plant oils contain biopesticides that could be used to control many crop pests. Tetranychus spp. are mites that cause damage to several crops and are primarily controlled by synthetic pesticides. Literature showed that mites can be controlled with essential oils of plants containing eugenol. In this work, we evaluated the bioactivity of basil (Ocimum basicilum) accessions for peanut-spider mites control based on molecular, biochemical and agronomic assays. RNA from four basil accessions, previously chosen by divergence genetic analysis, were used to estimate the expression of eugenol synthase (EGS I) transcripts, by semiquantitative and polymerase chain reaction (qPCR) assays. Chromatography was, thereafter, performed in order to estimate the eugenol concentration. Feeding bioassays were performed using basil leaf extracts in order to estimate oviposition and mortality of spider mites females. Finally, a validation assay was carried out in greenhouse, using peanut plants previously infested with spider mites and weekly sprayed with basil water-extract. One basil accession, OVRS, revealed high phytotoxicity to spider mite females, at 15% water-extract. The mortality rate was 75% and complete inhibition of fecundity was found in BOD assays. In the greenhouse assay, the most severe damage due to mite infestations was found to plant height, number of pods and pod yield, which were reduced to 28, 53 and 52% in non-treated plants (control). Considering that basil is a short-cycle plant, with easy reproduction and management, these results represent an accessible alternative to organic control spider mites in peanut. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-03-03T23:57:51Z 2017-03-03T23:57:51Z 2017-03-03 2017-03-03T23:57:51Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
African Journal of Biotechnology, v. 15, n. 30, p. 1597-1607, July 2016. 1684-5315 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065905 |
identifier_str_mv |
African Journal of Biotechnology, v. 15, n. 30, p. 1597-1607, July 2016. 1684-5315 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1065905 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503433488695296 |