Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.

Detalhes bibliográficos
Autor(a) principal: FAJARDO, T. V. M.
Data de Publicação: 2020
Outros Autores: Bertocchi, A. A., NICKEL, O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122194
Resumo: Among grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; survey
id EMBR_4a74bff9db0c21cff14c5e6f63e4cc86
oai_identifier_str oai:www.alice.cnptia.embrapa.br:doc/1122194
network_acronym_str EMBR
network_name_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository_id_str 2154
spelling Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.RT-qPCRHTSNGSIndexingSurveyGrapevine (Vitis spp)VitisPathogensVirusesAmong grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; surveyTHOR VINICIUS MARTINS FAJARDO, CNPUV; Airton Alexandre Bertocchi, Universidade Estadual do Rio Grande do Sul, Bento Gonçalves, Rio Grande do Sul, Brazil. airtonalebertocchi@gmail.com; OSMAR NICKEL, CNPUV.FAJARDO, T. V. M.Bertocchi, A. A.NICKEL, O.2020-05-12T04:39:33Z2020-05-12T04:39:33Z2020-05-112020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Ceres, Viçosa, v. 67, n. 2, p. 156-163, mar./apr. 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/112219410.1590/0034-737X202067020010enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-05-12T04:39:39Zoai:www.alice.cnptia.embrapa.br:doc/1122194Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-05-12T04:39:39falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-05-12T04:39:39Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
title Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
spellingShingle Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
FAJARDO, T. V. M.
RT-qPCR
HTS
NGS
Indexing
Survey
Grapevine (Vitis spp)
Vitis
Pathogens
Viruses
title_short Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
title_full Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
title_fullStr Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
title_full_unstemmed Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
title_sort Determination of the grapevine virome by high-throughput sequencing and grapevine viruses detection in Serra Gaúcha, Brazil.
author FAJARDO, T. V. M.
author_facet FAJARDO, T. V. M.
Bertocchi, A. A.
NICKEL, O.
author_role author
author2 Bertocchi, A. A.
NICKEL, O.
author2_role author
author
dc.contributor.none.fl_str_mv THOR VINICIUS MARTINS FAJARDO, CNPUV; Airton Alexandre Bertocchi, Universidade Estadual do Rio Grande do Sul, Bento Gonçalves, Rio Grande do Sul, Brazil. airtonalebertocchi@gmail.com; OSMAR NICKEL, CNPUV.
dc.contributor.author.fl_str_mv FAJARDO, T. V. M.
Bertocchi, A. A.
NICKEL, O.
dc.subject.por.fl_str_mv RT-qPCR
HTS
NGS
Indexing
Survey
Grapevine (Vitis spp)
Vitis
Pathogens
Viruses
topic RT-qPCR
HTS
NGS
Indexing
Survey
Grapevine (Vitis spp)
Vitis
Pathogens
Viruses
description Among grapevine (Vitis spp.) pathogens, viruses stand out, considering that this fruit plant is broadly susceptible to them and that they cause several diseases, reducing quality and yield of grape production. However, detecting and identifying viral infections in grapevines can be challenging. The objectives of this study were to detect grapevine viral pathogens by high-throughput sequencing (HTS) and identify grapevine viruses associated with plant propagation material from different origins. A comprehensive picture of a vine exhibiting virus-related symptoms was generated. The detected virome included four different pathogens and their variants. In addition, the incidence of seven viral pathogens was determined in samples collected in three vineyards of the main grape-growing region of Brazil. It is suggested that the observed infections could be related to the technological level of the production system adopted by these grape growers. Keywords: RT-qPCR; HTS; NGS; Vitis; indexing; survey
publishDate 2020
dc.date.none.fl_str_mv 2020-05-12T04:39:33Z
2020-05-12T04:39:33Z
2020-05-11
2020
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Ceres, Viçosa, v. 67, n. 2, p. 156-163, mar./apr. 2020.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122194
10.1590/0034-737X202067020010
identifier_str_mv Revista Ceres, Viçosa, v. 67, n. 2, p. 156-163, mar./apr. 2020.
10.1590/0034-737X202067020010
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1122194
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
_version_ 1794503492237262848

Registros relacionados