Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1128105 |
Resumo: | The banana tree is one of the most cultivated fruit globally; however, some diseases significantly affect its production, such as Fusarium wilt. The most appropriate measure for controlling this disease in areas with inoculum pressure is the use of resistant cultivars. Therefore, this study aimed to generate banana somaclones of the cultivar ?Prata-Anã? resistant to Fusarium wilt by inducing somaclonal variation. ?Prata-Anã? stem apexes were established in vitro in MS culture medium and, on a monthly basis, subcultivated in AIA and adenine sulfate supplemented MS medium with added plant regulators: 6-benzylaminopurine (BAP, 4 ml L-1), Thidiazuron (TDZ, 1 ml L-1), and Paclobutrazol (PBZ, 10 ml L-1). The treatments were: T0: no regulator, T1: BAP, T2: TDZ, T3: PBZ, T4: BAP + TDZ, T5: BAP + PBZ, T6: TDZ + PBZ, and T7: BAP + TDZ + PBZ. After the twelfth subculture, the regenerated plants were planted in boxes containing sterile soil infected with Fusarium oxysporum f. sp. cubense, and evaluated after 90 days for resistance to the pathogen. Somaclonal variants T2-1 and T2-2, generated in Treatment 2, with TDZ, were selected as resistant. This result is promising for the launch of a new Fusarium race 1-resistant banana variety. |
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Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race.BananaDoença de PlantaThe banana tree is one of the most cultivated fruit globally; however, some diseases significantly affect its production, such as Fusarium wilt. The most appropriate measure for controlling this disease in areas with inoculum pressure is the use of resistant cultivars. Therefore, this study aimed to generate banana somaclones of the cultivar ?Prata-Anã? resistant to Fusarium wilt by inducing somaclonal variation. ?Prata-Anã? stem apexes were established in vitro in MS culture medium and, on a monthly basis, subcultivated in AIA and adenine sulfate supplemented MS medium with added plant regulators: 6-benzylaminopurine (BAP, 4 ml L-1), Thidiazuron (TDZ, 1 ml L-1), and Paclobutrazol (PBZ, 10 ml L-1). The treatments were: T0: no regulator, T1: BAP, T2: TDZ, T3: PBZ, T4: BAP + TDZ, T5: BAP + PBZ, T6: TDZ + PBZ, and T7: BAP + TDZ + PBZ. After the twelfth subculture, the regenerated plants were planted in boxes containing sterile soil infected with Fusarium oxysporum f. sp. cubense, and evaluated after 90 days for resistance to the pathogen. Somaclonal variants T2-1 and T2-2, generated in Treatment 2, with TDZ, were selected as resistant. This result is promising for the launch of a new Fusarium race 1-resistant banana variety.MILEIDE DOS SANTOS FERREIRA, UFRB; ÉRICA RODRIGUES DE MOURA, UFRB; LUCYMEIRE SOUZA MORAIS LINO; EDSON PERITO AMORIM, CNPMF; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; FERNANDO HADDAD, CNPMF.FERREIRA, M. dos S.MOURA, É. R. deLINO, L. S. M.AMORIM, E. P.SEREJO, J. A. dos S.HADDAD, F.2020-12-16T09:02:44Z2020-12-16T09:02:44Z2020-12-142020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Fruticultura, Jaboticabal, v.42, n.6, 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1128105porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-12-16T09:02:51Zoai:www.alice.cnptia.embrapa.br:doc/1128105Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-12-16T09:02:51falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-12-16T09:02:51Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race. |
title |
Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race. |
spellingShingle |
Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race. FERREIRA, M. dos S. Banana Doença de Planta |
title_short |
Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race. |
title_full |
Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race. |
title_fullStr |
Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race. |
title_full_unstemmed |
Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race. |
title_sort |
Selection of somaclonal variants of the cultivar 'Prata-Anã' for resistance to Fusarium Oxysporum F. sp. Cubense Race. |
author |
FERREIRA, M. dos S. |
author_facet |
FERREIRA, M. dos S. MOURA, É. R. de LINO, L. S. M. AMORIM, E. P. SEREJO, J. A. dos S. HADDAD, F. |
author_role |
author |
author2 |
MOURA, É. R. de LINO, L. S. M. AMORIM, E. P. SEREJO, J. A. dos S. HADDAD, F. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
MILEIDE DOS SANTOS FERREIRA, UFRB; ÉRICA RODRIGUES DE MOURA, UFRB; LUCYMEIRE SOUZA MORAIS LINO; EDSON PERITO AMORIM, CNPMF; JANAY ALMEIDA DOS SANTOS SEREJO, CNPMF; FERNANDO HADDAD, CNPMF. |
dc.contributor.author.fl_str_mv |
FERREIRA, M. dos S. MOURA, É. R. de LINO, L. S. M. AMORIM, E. P. SEREJO, J. A. dos S. HADDAD, F. |
dc.subject.por.fl_str_mv |
Banana Doença de Planta |
topic |
Banana Doença de Planta |
description |
The banana tree is one of the most cultivated fruit globally; however, some diseases significantly affect its production, such as Fusarium wilt. The most appropriate measure for controlling this disease in areas with inoculum pressure is the use of resistant cultivars. Therefore, this study aimed to generate banana somaclones of the cultivar ?Prata-Anã? resistant to Fusarium wilt by inducing somaclonal variation. ?Prata-Anã? stem apexes were established in vitro in MS culture medium and, on a monthly basis, subcultivated in AIA and adenine sulfate supplemented MS medium with added plant regulators: 6-benzylaminopurine (BAP, 4 ml L-1), Thidiazuron (TDZ, 1 ml L-1), and Paclobutrazol (PBZ, 10 ml L-1). The treatments were: T0: no regulator, T1: BAP, T2: TDZ, T3: PBZ, T4: BAP + TDZ, T5: BAP + PBZ, T6: TDZ + PBZ, and T7: BAP + TDZ + PBZ. After the twelfth subculture, the regenerated plants were planted in boxes containing sterile soil infected with Fusarium oxysporum f. sp. cubense, and evaluated after 90 days for resistance to the pathogen. Somaclonal variants T2-1 and T2-2, generated in Treatment 2, with TDZ, were selected as resistant. This result is promising for the launch of a new Fusarium race 1-resistant banana variety. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-16T09:02:44Z 2020-12-16T09:02:44Z 2020-12-14 2020 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Fruticultura, Jaboticabal, v.42, n.6, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1128105 |
identifier_str_mv |
Revista Brasileira de Fruticultura, Jaboticabal, v.42, n.6, 2020. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1128105 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503499299422208 |