Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.

Detalhes bibliográficos
Autor(a) principal: NOGUEIRA, R. T.
Data de Publicação: 2002
Outros Autores: QUEIROZ, S. C. do N. de, IMAMURA, P. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/15040
Resumo: Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS).
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spelling Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.CromatografiaHymenaea CourbarilColumn chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS).R. T. NOGUEIRA, IQ/Unicamp; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; P. M. IMAMURA, IQ/Unicamp.NOGUEIRA, R. T.QUEIROZ, S. C. do N. deIMAMURA, P. M.2017-09-19T00:11:49Z2017-09-19T00:11:49Z2006-09-2920022017-09-20T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Liquid Chromatography & Related Technologies, New York, v. 25, p 59-67, 2002.http://www.alice.cnptia.embrapa.br/alice/handle/doc/15040enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-09-19T00:11:49Zoai:www.alice.cnptia.embrapa.br:doc/15040Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-09-19T00:11:49falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-09-19T00:11:49Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
title Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
spellingShingle Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
NOGUEIRA, R. T.
Cromatografia
Hymenaea Courbaril
title_short Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
title_full Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
title_fullStr Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
title_full_unstemmed Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
title_sort Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
author NOGUEIRA, R. T.
author_facet NOGUEIRA, R. T.
QUEIROZ, S. C. do N. de
IMAMURA, P. M.
author_role author
author2 QUEIROZ, S. C. do N. de
IMAMURA, P. M.
author2_role author
author
dc.contributor.none.fl_str_mv R. T. NOGUEIRA, IQ/Unicamp; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; P. M. IMAMURA, IQ/Unicamp.
dc.contributor.author.fl_str_mv NOGUEIRA, R. T.
QUEIROZ, S. C. do N. de
IMAMURA, P. M.
dc.subject.por.fl_str_mv Cromatografia
Hymenaea Courbaril
topic Cromatografia
Hymenaea Courbaril
description Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS).
publishDate 2002
dc.date.none.fl_str_mv 2002
2006-09-29
2017-09-19T00:11:49Z
2017-09-19T00:11:49Z
2017-09-20T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Journal of Liquid Chromatography & Related Technologies, New York, v. 25, p 59-67, 2002.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/15040
identifier_str_mv Journal of Liquid Chromatography & Related Technologies, New York, v. 25, p 59-67, 2002.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/15040
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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