Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines.
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/480546 |
Resumo: | The effects of a short-term (80 min) exposure to 222 _uM aluminum (AI) on the protein content and expression and on peroxidase activity and isoenzymes in the primary root of maize were evaluated. Two inbred lines differing in their leveI of tolerance to AI were used: Cateto 237 (tolerant) and L36 (sensitive). The apical 20 mm of the primary root was divided into 2-mm-Iong segments that were analyzed for total protein content and peroxidase activity. These results demonstrate that the total protein content along the root apex was not affected by AI in the tolerant inbred line, but decreased in the sensitive line. In the apical 2 mm of the root of the sensitive line, the expression of low molecular weight proteins (43 kDa or smaller) was decreased. Expression of low molecular proteins increased in the tolerant inbred line, even though total protein content did not increase. This suggests that some of these proteins could play a role in metal tolerance, perhaps as binding peptides. While the peroxidase activity of the tolerant inbred line did not change with exposure to AI, peroxidase activity in the apical 6 mm of the root of the sensitive line decreased. The tolerant inbred line constitutively expressed more anionic peroxidase isoforms. These results demonstrate that maintenance of protein expression may be an important component of the plant's resistance to AI stress, and that resistance to AI stress is associated with the higher expression of anionic peroxidase isoforms. |
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Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines.Crescimento radicularIsoformas de peroxidaseToxicidade do alumínioMilhoThe effects of a short-term (80 min) exposure to 222 _uM aluminum (AI) on the protein content and expression and on peroxidase activity and isoenzymes in the primary root of maize were evaluated. Two inbred lines differing in their leveI of tolerance to AI were used: Cateto 237 (tolerant) and L36 (sensitive). The apical 20 mm of the primary root was divided into 2-mm-Iong segments that were analyzed for total protein content and peroxidase activity. These results demonstrate that the total protein content along the root apex was not affected by AI in the tolerant inbred line, but decreased in the sensitive line. In the apical 2 mm of the root of the sensitive line, the expression of low molecular weight proteins (43 kDa or smaller) was decreased. Expression of low molecular proteins increased in the tolerant inbred line, even though total protein content did not increase. This suggests that some of these proteins could play a role in metal tolerance, perhaps as binding peptides. While the peroxidase activity of the tolerant inbred line did not change with exposure to AI, peroxidase activity in the apical 6 mm of the root of the sensitive line decreased. The tolerant inbred line constitutively expressed more anionic peroxidase isoforms. These results demonstrate that maintenance of protein expression may be an important component of the plant's resistance to AI stress, and that resistance to AI stress is associated with the higher expression of anionic peroxidase isoforms.ISABEL REGINA PRAZERES DE SOUZA, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS; SIDNEY NETTO PARENTONI, CNPMS; ANTONIO CARLOS DE OLIVEIRA, CNPMS; FLAVIA FRANCA TEIXEIRA, CNPMS.SOUZA, I. R. P. deALVES, V. M. C.PARENTONI, S. N.OLIVEIRA, A. C. deTEIXEIRA, F. F.ADAM, J. W. Mac.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2003-03-0520022018-06-07T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrazilian Journal of Plant Physiology, Londrina, v. 14, n. 3, p. 219-224, 2002.http://www.alice.cnptia.embrapa.br/alice/handle/doc/480546enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:33:16Zoai:www.alice.cnptia.embrapa.br:doc/480546Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:33:16falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:33:16Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. |
title |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. |
spellingShingle |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. SOUZA, I. R. P. de Crescimento radicular Isoformas de peroxidase Toxicidade do alumínio Milho |
title_short |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. |
title_full |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. |
title_fullStr |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. |
title_full_unstemmed |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. |
title_sort |
Change in root apical protein and peroxidase activity in response to aluminum in tolerant and sensitive maize inbred lines. |
author |
SOUZA, I. R. P. de |
author_facet |
SOUZA, I. R. P. de ALVES, V. M. C. PARENTONI, S. N. OLIVEIRA, A. C. de TEIXEIRA, F. F. ADAM, J. W. Mac. |
author_role |
author |
author2 |
ALVES, V. M. C. PARENTONI, S. N. OLIVEIRA, A. C. de TEIXEIRA, F. F. ADAM, J. W. Mac. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
ISABEL REGINA PRAZERES DE SOUZA, CNPMS; VERA MARIA CARVALHO ALVES, CNPMS; SIDNEY NETTO PARENTONI, CNPMS; ANTONIO CARLOS DE OLIVEIRA, CNPMS; FLAVIA FRANCA TEIXEIRA, CNPMS. |
dc.contributor.author.fl_str_mv |
SOUZA, I. R. P. de ALVES, V. M. C. PARENTONI, S. N. OLIVEIRA, A. C. de TEIXEIRA, F. F. ADAM, J. W. Mac. |
dc.subject.por.fl_str_mv |
Crescimento radicular Isoformas de peroxidase Toxicidade do alumínio Milho |
topic |
Crescimento radicular Isoformas de peroxidase Toxicidade do alumínio Milho |
description |
The effects of a short-term (80 min) exposure to 222 _uM aluminum (AI) on the protein content and expression and on peroxidase activity and isoenzymes in the primary root of maize were evaluated. Two inbred lines differing in their leveI of tolerance to AI were used: Cateto 237 (tolerant) and L36 (sensitive). The apical 20 mm of the primary root was divided into 2-mm-Iong segments that were analyzed for total protein content and peroxidase activity. These results demonstrate that the total protein content along the root apex was not affected by AI in the tolerant inbred line, but decreased in the sensitive line. In the apical 2 mm of the root of the sensitive line, the expression of low molecular weight proteins (43 kDa or smaller) was decreased. Expression of low molecular proteins increased in the tolerant inbred line, even though total protein content did not increase. This suggests that some of these proteins could play a role in metal tolerance, perhaps as binding peptides. While the peroxidase activity of the tolerant inbred line did not change with exposure to AI, peroxidase activity in the apical 6 mm of the root of the sensitive line decreased. The tolerant inbred line constitutively expressed more anionic peroxidase isoforms. These results demonstrate that maintenance of protein expression may be an important component of the plant's resistance to AI stress, and that resistance to AI stress is associated with the higher expression of anionic peroxidase isoforms. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002 2003-03-05 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2018-06-07T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Plant Physiology, Londrina, v. 14, n. 3, p. 219-224, 2002. http://www.alice.cnptia.embrapa.br/alice/handle/doc/480546 |
identifier_str_mv |
Brazilian Journal of Plant Physiology, Londrina, v. 14, n. 3, p. 219-224, 2002. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/480546 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503341409042432 |