Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138005 |
Resumo: | Thymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH?), hydroxyl (?OH), and nitric oxide (?NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for ?NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents. |
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Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth.Thymus serrulatusAntioxidanteExtrato VegetalExtratoÓleo EssencialThymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH?), hydroxyl (?OH), and nitric oxide (?NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for ?NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents.TESFAY HAILE, Mekelle UniversitySUSANA M. CARDOSOCHIRLE DE OLIVEIRA RAPHAELLIOLÍVIA R. PEREIRAELISA DOS SANTOS PEREIRAMARCIA VIZZOTTO, CPACTLEONARDO NORAADISSU ALEMAYEHU ASFAW, Mekelle UniversityGOMATHI PERIASAMY, Mekelle UniversityAMAN KARIM, Mekelle University.HAILE, T.CARDOSO, S. M.RAPHAELLI, C. de O.PEREIRA, O. R.PEREIRA, E. dos S.VIZZOTTO, M.NORA, L.ASFAW, A. A.PERIASAMY, G.KARIM, A.2021-12-17T02:05:35Z2021-12-17T02:05:35Z2021-12-162021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFrontiers in Pharmacology, v. 12, article 621536, April 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138005porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-12-17T02:05:44Zoai:www.alice.cnptia.embrapa.br:doc/1138005Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-12-17T02:05:44falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-12-17T02:05:44Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. |
title |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. |
spellingShingle |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. HAILE, T. Thymus serrulatus Antioxidante Extrato Vegetal Extrato Óleo Essencial |
title_short |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. |
title_full |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. |
title_fullStr |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. |
title_full_unstemmed |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. |
title_sort |
Chemical Composition, Antioxidant Potential, and Blood Glucose Lowering Effect of Aqueous Extract and Essential Oil of Thymus Serrulatus Hochst. Ex Benth. |
author |
HAILE, T. |
author_facet |
HAILE, T. CARDOSO, S. M. RAPHAELLI, C. de O. PEREIRA, O. R. PEREIRA, E. dos S. VIZZOTTO, M. NORA, L. ASFAW, A. A. PERIASAMY, G. KARIM, A. |
author_role |
author |
author2 |
CARDOSO, S. M. RAPHAELLI, C. de O. PEREIRA, O. R. PEREIRA, E. dos S. VIZZOTTO, M. NORA, L. ASFAW, A. A. PERIASAMY, G. KARIM, A. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
TESFAY HAILE, Mekelle University SUSANA M. CARDOSO CHIRLE DE OLIVEIRA RAPHAELLI OLÍVIA R. PEREIRA ELISA DOS SANTOS PEREIRA MARCIA VIZZOTTO, CPACT LEONARDO NORA ADISSU ALEMAYEHU ASFAW, Mekelle University GOMATHI PERIASAMY, Mekelle University AMAN KARIM, Mekelle University. |
dc.contributor.author.fl_str_mv |
HAILE, T. CARDOSO, S. M. RAPHAELLI, C. de O. PEREIRA, O. R. PEREIRA, E. dos S. VIZZOTTO, M. NORA, L. ASFAW, A. A. PERIASAMY, G. KARIM, A. |
dc.subject.por.fl_str_mv |
Thymus serrulatus Antioxidante Extrato Vegetal Extrato Óleo Essencial |
topic |
Thymus serrulatus Antioxidante Extrato Vegetal Extrato Óleo Essencial |
description |
Thymus serrulatus, an endemic plant of Ethiopia, is traditionally used to cure various diseases and as a food ingredient. In the Ethiopian folk medicine, the decoction is orally taken as a remedy to treat diabetes and high blood pressure. The purpose of the present study was to evaluate the antioxidant and antihyperglycemic effects of the aqueous extract and of the essential oil of Thymus serrulatus. The chemical composition of the aqueous extract was determined by LC-MS and the essential oil was characterized by GC-MS analysis. Radical scavenging assays, namely scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH?), hydroxyl (?OH), and nitric oxide (?NO), were used as a first approach to screen the potential antioxidant abilities of the samples. Alpha-amylase and α-glucosidase inhibitory studies were also employed to evaluate the in vitro antihyperglycemic potential of the plant. The in vivo blood glucose lowering effect of the extracts was assessed using hypoglycemic activity and the oral glucose tolerance test in normal and in streptozotocin induced diabetic mice. When compared to the aqueous extract, the essential oil showed superior radical scavenging activity, particularly for ?NO, as well as greater inhibitory potency against α-amylase and α-glucosidase (IC50 0.01 mg/ml and 0.11 mg/ml, respectively). Both tested samples showed a statistically significant antihyperglycemic effect. The aqueous extract at 600 mg/kg exerted maximum antihyperglycemic activity (44.14%), followed by the essential oil (30.82%). Body weight and glucose tolerance parameters were also improved by the samples both in normal and diabetic mice. The findings of this study support the hypothesis that aqueous extract and essential oil of T. serrulatus are promising therapeutic agents. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-17T02:05:35Z 2021-12-17T02:05:35Z 2021-12-16 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Frontiers in Pharmacology, v. 12, article 621536, April 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138005 |
identifier_str_mv |
Frontiers in Pharmacology, v. 12, article 621536, April 2021. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138005 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503515052179456 |