Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.

Detalhes bibliográficos
Autor(a) principal: MANTELLATTO, A. M. B.
Data de Publicação: 2017
Outros Autores: CAPARROZ, R., CHRISTOFOLETTI, M. D., PIOVEZAN, U., DUARTE, J. M. B.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080689
http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323
Resumo: The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.
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spelling Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.VeadoOzotoceros BezoarticusDeerThe pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.ALINE MEIRA BONFIM MANTELLATTO, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); RENATO CAPARROZ, Universidade de Brasília; MAURÍCIO DURANTE CHRISTOFOLETTI, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); UBIRATAN PIOVEZAN, CPATC; JOSÉ MAURÍCIO BARBANTI DUARTE, Universidade de Brasília.MANTELLATTO, A. M. B.CAPARROZ, R.CHRISTOFOLETTI, M. D.PIOVEZAN, U.DUARTE, J. M. B.2017-11-24T23:22:22Z2017-11-24T23:22:22Z2017-11-2420172017-11-24T23:22:22Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080689http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-11-24T23:22:28Zoai:www.alice.cnptia.embrapa.br:doc/1080689Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-11-24T23:22:28falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-11-24T23:22:28Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
title Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
spellingShingle Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
MANTELLATTO, A. M. B.
Veado
Ozotoceros Bezoarticus
Deer
title_short Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
title_full Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
title_fullStr Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
title_full_unstemmed Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
title_sort Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
author MANTELLATTO, A. M. B.
author_facet MANTELLATTO, A. M. B.
CAPARROZ, R.
CHRISTOFOLETTI, M. D.
PIOVEZAN, U.
DUARTE, J. M. B.
author_role author
author2 CAPARROZ, R.
CHRISTOFOLETTI, M. D.
PIOVEZAN, U.
DUARTE, J. M. B.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv ALINE MEIRA BONFIM MANTELLATTO, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); RENATO CAPARROZ, Universidade de Brasília; MAURÍCIO DURANTE CHRISTOFOLETTI, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); UBIRATAN PIOVEZAN, CPATC; JOSÉ MAURÍCIO BARBANTI DUARTE, Universidade de Brasília.
dc.contributor.author.fl_str_mv MANTELLATTO, A. M. B.
CAPARROZ, R.
CHRISTOFOLETTI, M. D.
PIOVEZAN, U.
DUARTE, J. M. B.
dc.subject.por.fl_str_mv Veado
Ozotoceros Bezoarticus
Deer
topic Veado
Ozotoceros Bezoarticus
Deer
description The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-24T23:22:22Z
2017-11-24T23:22:22Z
2017-11-24
2017
2017-11-24T23:22:22Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080689
http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323
identifier_str_mv Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080689
http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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