Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080689 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323 |
Resumo: | The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability. |
id |
EMBR_4fd57dd2ee2f98a4e513ecd321db842e |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1080689 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.VeadoOzotoceros BezoarticusDeerThe pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.ALINE MEIRA BONFIM MANTELLATTO, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); RENATO CAPARROZ, Universidade de Brasília; MAURÍCIO DURANTE CHRISTOFOLETTI, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); UBIRATAN PIOVEZAN, CPATC; JOSÉ MAURÍCIO BARBANTI DUARTE, Universidade de Brasília.MANTELLATTO, A. M. B.CAPARROZ, R.CHRISTOFOLETTI, M. D.PIOVEZAN, U.DUARTE, J. M. B.2017-11-24T23:22:22Z2017-11-24T23:22:22Z2017-11-2420172017-11-24T23:22:22Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080689http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-11-24T23:22:28Zoai:www.alice.cnptia.embrapa.br:doc/1080689Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-11-24T23:22:28falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-11-24T23:22:28Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
spellingShingle |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. MANTELLATTO, A. M. B. Veado Ozotoceros Bezoarticus Deer |
title_short |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_full |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_fullStr |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_full_unstemmed |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_sort |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
author |
MANTELLATTO, A. M. B. |
author_facet |
MANTELLATTO, A. M. B. CAPARROZ, R. CHRISTOFOLETTI, M. D. PIOVEZAN, U. DUARTE, J. M. B. |
author_role |
author |
author2 |
CAPARROZ, R. CHRISTOFOLETTI, M. D. PIOVEZAN, U. DUARTE, J. M. B. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
ALINE MEIRA BONFIM MANTELLATTO, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); RENATO CAPARROZ, Universidade de Brasília; MAURÍCIO DURANTE CHRISTOFOLETTI, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); UBIRATAN PIOVEZAN, CPATC; JOSÉ MAURÍCIO BARBANTI DUARTE, Universidade de Brasília. |
dc.contributor.author.fl_str_mv |
MANTELLATTO, A. M. B. CAPARROZ, R. CHRISTOFOLETTI, M. D. PIOVEZAN, U. DUARTE, J. M. B. |
dc.subject.por.fl_str_mv |
Veado Ozotoceros Bezoarticus Deer |
topic |
Veado Ozotoceros Bezoarticus Deer |
description |
The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-24T23:22:22Z 2017-11-24T23:22:22Z 2017-11-24 2017 2017-11-24T23:22:22Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080689 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323 |
identifier_str_mv |
Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080689 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503445147811840 |