Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/987833 |
Resumo: | Six endophytic bacteria of corn roots were identified as Bacillus sp. and as Enterobacter sp, by sequencing of the 16S rRNA gene. Four of the strains, CNPSo 2476, CNPSo 2477, CNPSo 2478 and CNPSo 2480 were positive for the nitrogen fixation ability evaluated through the acetylene reduction assay and amplification of nifH gene. Two Bacillus strains (CNPSo 2477 and CNPSo 2478) showed outstanding skills for the production of IAA, siderophores and lytic enzymes, but were not good candidates as growth promoters, because they reduced seed germination. However, the same strains were antagonists against the pathogenic fungi Fusarium verticillioides, Colletotrichum graminicola, Bipolaris maydis and Cercospora zea-maydis. As an indication of favorable bacterial action, Enterobacter sp. CNPSo 2480 and Bacillus sp. CNPSo 2481 increased the root volume by 44% and 39%, respectively, and the seed germination by 47% and 56%, respectively. Therefore, these two strains are good candidates for future testing as biological inoculants for corn. |
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Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture.MilhoSix endophytic bacteria of corn roots were identified as Bacillus sp. and as Enterobacter sp, by sequencing of the 16S rRNA gene. Four of the strains, CNPSo 2476, CNPSo 2477, CNPSo 2478 and CNPSo 2480 were positive for the nitrogen fixation ability evaluated through the acetylene reduction assay and amplification of nifH gene. Two Bacillus strains (CNPSo 2477 and CNPSo 2478) showed outstanding skills for the production of IAA, siderophores and lytic enzymes, but were not good candidates as growth promoters, because they reduced seed germination. However, the same strains were antagonists against the pathogenic fungi Fusarium verticillioides, Colletotrichum graminicola, Bipolaris maydis and Cercospora zea-maydis. As an indication of favorable bacterial action, Enterobacter sp. CNPSo 2480 and Bacillus sp. CNPSo 2481 increased the root volume by 44% and 39%, respectively, and the seed germination by 47% and 56%, respectively. Therefore, these two strains are good candidates for future testing as biological inoculants for corn.VIVIAN JASKIW SZILAGYI-ZECCHIN, UFPR; ANGELA CRISTINA IKEDA, UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO; DOUGLAS ADAMOSKI, UFPR; VANESSA KAVA-CORDEIRO, UFPR; CHIRLEI GLIENKE, UFPR; LYGIA VITÓRIA GALLI-TERASAWA, UFPR.SZILAGYI-ZECCHIN, V. J.IKEDA, A. C.HUNGRIA, M.ADAMOSKI, D.KAVA-CORDEIRO, V.GLIENKE, C.GALLI-TERASAWA, L. V.2014-06-06T11:11:11Z2014-06-06T11:11:11Z2014-06-0620142015-01-06T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAMB Express, Heidelberg, v. 4, n. 26, p. 1-9, May, 2014.2191-0855http://www.alice.cnptia.embrapa.br/alice/handle/doc/98783310.1186/s13568-014-0026-yenginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T01:59:10Zoai:www.alice.cnptia.embrapa.br:doc/987833Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T01:59:10falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T01:59:10Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture. |
title |
Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture. |
spellingShingle |
Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture. SZILAGYI-ZECCHIN, V. J. Milho |
title_short |
Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture. |
title_full |
Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture. |
title_fullStr |
Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture. |
title_full_unstemmed |
Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture. |
title_sort |
Identification and characterization of endophytic bacteria from corn (Zea mays L.) roots with biotechnological potential in agriculture. |
author |
SZILAGYI-ZECCHIN, V. J. |
author_facet |
SZILAGYI-ZECCHIN, V. J. IKEDA, A. C. HUNGRIA, M. ADAMOSKI, D. KAVA-CORDEIRO, V. GLIENKE, C. GALLI-TERASAWA, L. V. |
author_role |
author |
author2 |
IKEDA, A. C. HUNGRIA, M. ADAMOSKI, D. KAVA-CORDEIRO, V. GLIENKE, C. GALLI-TERASAWA, L. V. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
VIVIAN JASKIW SZILAGYI-ZECCHIN, UFPR; ANGELA CRISTINA IKEDA, UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO; DOUGLAS ADAMOSKI, UFPR; VANESSA KAVA-CORDEIRO, UFPR; CHIRLEI GLIENKE, UFPR; LYGIA VITÓRIA GALLI-TERASAWA, UFPR. |
dc.contributor.author.fl_str_mv |
SZILAGYI-ZECCHIN, V. J. IKEDA, A. C. HUNGRIA, M. ADAMOSKI, D. KAVA-CORDEIRO, V. GLIENKE, C. GALLI-TERASAWA, L. V. |
dc.subject.por.fl_str_mv |
Milho |
topic |
Milho |
description |
Six endophytic bacteria of corn roots were identified as Bacillus sp. and as Enterobacter sp, by sequencing of the 16S rRNA gene. Four of the strains, CNPSo 2476, CNPSo 2477, CNPSo 2478 and CNPSo 2480 were positive for the nitrogen fixation ability evaluated through the acetylene reduction assay and amplification of nifH gene. Two Bacillus strains (CNPSo 2477 and CNPSo 2478) showed outstanding skills for the production of IAA, siderophores and lytic enzymes, but were not good candidates as growth promoters, because they reduced seed germination. However, the same strains were antagonists against the pathogenic fungi Fusarium verticillioides, Colletotrichum graminicola, Bipolaris maydis and Cercospora zea-maydis. As an indication of favorable bacterial action, Enterobacter sp. CNPSo 2480 and Bacillus sp. CNPSo 2481 increased the root volume by 44% and 39%, respectively, and the seed germination by 47% and 56%, respectively. Therefore, these two strains are good candidates for future testing as biological inoculants for corn. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-06T11:11:11Z 2014-06-06T11:11:11Z 2014-06-06 2014 2015-01-06T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
AMB Express, Heidelberg, v. 4, n. 26, p. 1-9, May, 2014. 2191-0855 http://www.alice.cnptia.embrapa.br/alice/handle/doc/987833 10.1186/s13568-014-0026-y |
identifier_str_mv |
AMB Express, Heidelberg, v. 4, n. 26, p. 1-9, May, 2014. 2191-0855 10.1186/s13568-014-0026-y |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/987833 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503391065407488 |