Amino acid sequences of proteins from Leptospira serovar pomona.

• Autor(a) principal: ALVES, F. S. F.
• Data de Publicação: 2000
• Outros Autores: LEFEBVRE, R. B., PROBERT, W.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/515320
• topic: Sequência; Sequence; Doença animal; Aminoácido; Proteína; Leptospira pomona; Leptospirose; Leptospira; Proteins; Amino acids; Animal diseases; Antigens

Abstracts: This report describes a partial amino acid sequences from three putative outer envelope proteins from Leptospira serovar pomona. In order to obtain internal fragments for protein sequencing, enzymatic and chemical digestion was performed. The enzyme clostripain was used to digest the proteins 32 and 45kDa. In situ digestion of 40kDa molecular weight protein was accomplished using cyanogen bromide. The 32kDa protein generated two fragments, one of 21kDa and another of 10kDa that yielded five residues. A fragment of 24 kDa that yielded nineteen residues of amino acids was obtained from 45 kDa protein. A fragment with a molecular weight of 20 kDa, yielding a twenty amino acids sequence from the 40kDa protein.