Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010348 |
Resumo: | Background- MWCNT and CNF are interesting NPs that possess great potential for applications in various fields such as water treatment, reinforcement materials and medical devices. However, the rapid dissemination of NPs can impact the environment and in the human health. Thus, the aim of this study was to evaluate the MWCNT and cotton CNF toxicological effects on freshwater green microalgae Chlorella vulgaris. Results- Exposure to MWCNT and cotton CNF led to reductions on algal growth and cell viability. NP exposure induced reactive oxygen species (ROS) production and a decreased of intracellular ATP levels. Addition of NPs further induced ultrastructural cell damage. MWCNTs penetrate the cell membrane and individual MWCNTs are seen in the cytoplasm while no evidence of cotton CNFs was found inside the cells. Cellular uptake of MWCNT was observed in algae cells cultured in BB medium, but cells cultured in Seine river water did not internalize MWCNTs. Conclusions- Under the conditions tested, such results confirmed that exposure to MWCNTs and to cotton CNFs affects cell viability and algal growth. |
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Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris.BioindicadorNanopartículaNanotoxicologiaUptakeMicroalgaBackground- MWCNT and CNF are interesting NPs that possess great potential for applications in various fields such as water treatment, reinforcement materials and medical devices. However, the rapid dissemination of NPs can impact the environment and in the human health. Thus, the aim of this study was to evaluate the MWCNT and cotton CNF toxicological effects on freshwater green microalgae Chlorella vulgaris. Results- Exposure to MWCNT and cotton CNF led to reductions on algal growth and cell viability. NP exposure induced reactive oxygen species (ROS) production and a decreased of intracellular ATP levels. Addition of NPs further induced ultrastructural cell damage. MWCNTs penetrate the cell membrane and individual MWCNTs are seen in the cytoplasm while no evidence of cotton CNFs was found inside the cells. Cellular uptake of MWCNT was observed in algae cells cultured in BB medium, but cells cultured in Seine river water did not internalize MWCNTs. Conclusions- Under the conditions tested, such results confirmed that exposure to MWCNTs and to cotton CNFs affects cell viability and algal growth.HUMBERTO DE MELLO BRANDAO, CNPGL.PEREIRA, M. MMOUTON, L.YÉPRÉMIAN, C.COUTÉ, A.LO, J.MARCONCINI, J. M.LADEIRA, L. O.RAPOSO, N. R. B.BRANDAO, H. de M.BRAYNER, R.2015-03-09T14:01:50Z2015-03-09T14:01:50Z2015-03-0220142015-03-09T14:01:50Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Nanobiotechnology, Londres v. 12, n. 15, p. 1-13, 2014.http://www.alice.cnptia.embrapa.br/alice/handle/doc/101034810.1186/1477-3155-12-15enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:06:12Zoai:www.alice.cnptia.embrapa.br:doc/1010348Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:06:12falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:06:12Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. |
title |
Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. |
spellingShingle |
Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. PEREIRA, M. M Bioindicador Nanopartícula Nanotoxicologia Uptake Microalga |
title_short |
Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. |
title_full |
Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. |
title_fullStr |
Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. |
title_full_unstemmed |
Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. |
title_sort |
Ecotoxicological effects of carbon nanotubes and cellulose nanofibers in Chlorella vulgaris. |
author |
PEREIRA, M. M |
author_facet |
PEREIRA, M. M MOUTON, L. YÉPRÉMIAN, C. COUTÉ, A. LO, J. MARCONCINI, J. M. LADEIRA, L. O. RAPOSO, N. R. B. BRANDAO, H. de M. BRAYNER, R. |
author_role |
author |
author2 |
MOUTON, L. YÉPRÉMIAN, C. COUTÉ, A. LO, J. MARCONCINI, J. M. LADEIRA, L. O. RAPOSO, N. R. B. BRANDAO, H. de M. BRAYNER, R. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
HUMBERTO DE MELLO BRANDAO, CNPGL. |
dc.contributor.author.fl_str_mv |
PEREIRA, M. M MOUTON, L. YÉPRÉMIAN, C. COUTÉ, A. LO, J. MARCONCINI, J. M. LADEIRA, L. O. RAPOSO, N. R. B. BRANDAO, H. de M. BRAYNER, R. |
dc.subject.por.fl_str_mv |
Bioindicador Nanopartícula Nanotoxicologia Uptake Microalga |
topic |
Bioindicador Nanopartícula Nanotoxicologia Uptake Microalga |
description |
Background- MWCNT and CNF are interesting NPs that possess great potential for applications in various fields such as water treatment, reinforcement materials and medical devices. However, the rapid dissemination of NPs can impact the environment and in the human health. Thus, the aim of this study was to evaluate the MWCNT and cotton CNF toxicological effects on freshwater green microalgae Chlorella vulgaris. Results- Exposure to MWCNT and cotton CNF led to reductions on algal growth and cell viability. NP exposure induced reactive oxygen species (ROS) production and a decreased of intracellular ATP levels. Addition of NPs further induced ultrastructural cell damage. MWCNTs penetrate the cell membrane and individual MWCNTs are seen in the cytoplasm while no evidence of cotton CNFs was found inside the cells. Cellular uptake of MWCNT was observed in algae cells cultured in BB medium, but cells cultured in Seine river water did not internalize MWCNTs. Conclusions- Under the conditions tested, such results confirmed that exposure to MWCNTs and to cotton CNFs affects cell viability and algal growth. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-03-09T14:01:50Z 2015-03-09T14:01:50Z 2015-03-02 2015-03-09T14:01:50Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Nanobiotechnology, Londres v. 12, n. 15, p. 1-13, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010348 10.1186/1477-3155-12-15 |
identifier_str_mv |
Journal of Nanobiotechnology, Londres v. 12, n. 15, p. 1-13, 2014. 10.1186/1477-3155-12-15 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1010348 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503404099207168 |