Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena.
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124870 https://doi.org/10.1186/s12870-020-02592-0 |
Resumo: | Background: Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat (Triticum aestivum L.) production. Few blast resistance (R) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat cultivar BR 18-Terena is considered one of the best sources of resistance to blast and has been widely used in Brazilian breeding programmes, however the underlying genetics of this resistance are unknown. Results: BR 18-Terena was used as the common parent in the development of two recombinant inbred line (RIL) F6 populations with the Brazilian cultivars Anahuac 75 and BRS 179. Populations were phenotyped for resistance at the seedling and heading stage using the sequenced MoT isolate BR32, with transgressive segregation being observed. Genetic maps containing 1779 and 1318 markers, were produced for the Anahuac 75 × BR 18-Terena and BR 18- Terena × BRS 179 populations, respectively. Five quantitative trait loci (QTL) associated with seedling resistance, on chromosomes 2B, 4B (2 QTL), 5A and 6A, were identified, as were four QTL associated with heading stage resistance (1A, 2B, 4A and 5A). Seedling and heading stage QTL did not co-locate, despite a significant positive correlation between these traits, indicating that resistance at these developmental stages is likely to be controlled by different genes. BR 18-Terena provided the resistant allele for six QTL, at both developmental stages, with the largest phenotypic effect conferred by a QTL being 24.8% suggesting that BR 18-Terena possesses quantitative resistance. Haplotype analysis of 100 Brazilian wheat cultivars indicates that 11.0% of cultivars already possess a BR 18-Terenalike haplotype for more than one of the identified heading stage QTL. Conclusions: This study suggests that BR 18-Terena possesses quantitative resistance to wheat blast, with nine QTL associated with resistance at either the seedling or heading stage being detected. Wheat blast resistance is also largely tissue-specific. Identification of durable quantitative resistances which can be combined with race-specific R gene-mediated resistance is critical to effectively control wheat blast. Collectively, this work facilitates markerassisted selection to develop new varieties for cultivation in regions at risk from this emerging disease. |
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Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena.Wheat blastSeedling resistanceHead resistanceSingle nucleotide polymorphism (SNP) genotypingTriticum AestivumMagnaporthe oryzaeQuantitative trait lociGenotypingBackground: Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat (Triticum aestivum L.) production. Few blast resistance (R) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat cultivar BR 18-Terena is considered one of the best sources of resistance to blast and has been widely used in Brazilian breeding programmes, however the underlying genetics of this resistance are unknown. Results: BR 18-Terena was used as the common parent in the development of two recombinant inbred line (RIL) F6 populations with the Brazilian cultivars Anahuac 75 and BRS 179. Populations were phenotyped for resistance at the seedling and heading stage using the sequenced MoT isolate BR32, with transgressive segregation being observed. Genetic maps containing 1779 and 1318 markers, were produced for the Anahuac 75 × BR 18-Terena and BR 18- Terena × BRS 179 populations, respectively. Five quantitative trait loci (QTL) associated with seedling resistance, on chromosomes 2B, 4B (2 QTL), 5A and 6A, were identified, as were four QTL associated with heading stage resistance (1A, 2B, 4A and 5A). Seedling and heading stage QTL did not co-locate, despite a significant positive correlation between these traits, indicating that resistance at these developmental stages is likely to be controlled by different genes. BR 18-Terena provided the resistant allele for six QTL, at both developmental stages, with the largest phenotypic effect conferred by a QTL being 24.8% suggesting that BR 18-Terena possesses quantitative resistance. Haplotype analysis of 100 Brazilian wheat cultivars indicates that 11.0% of cultivars already possess a BR 18-Terenalike haplotype for more than one of the identified heading stage QTL. Conclusions: This study suggests that BR 18-Terena possesses quantitative resistance to wheat blast, with nine QTL associated with resistance at either the seedling or heading stage being detected. Wheat blast resistance is also largely tissue-specific. Identification of durable quantitative resistances which can be combined with race-specific R gene-mediated resistance is critical to effectively control wheat blast. Collectively, this work facilitates markerassisted selection to develop new varieties for cultivation in regions at risk from this emerging disease.RACHEL R. GODDARD, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UKANDREW STEED, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UKCATHERINE CHINOY, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UKJÉSSICA ROSSET FERREIRA, Universidade de Passo Fundo, Passo Fundo, RS, BrazilPEDRO LUIZ SCHEEREN, CNPTJOAO LEODATO NUNES MACIEL, CNPTEDUARDO CAIERAO, CNPTGISELE ABIGAIL MONTAN TORRES, CNPTLUCIANO CONSOLI, CNPTFLAVIO MARTINS SANTANA, CNPTJOSE MAURICIO CUNHA FERNANDES, CNPTJames Simmonds, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UKCRISTOBAL UAUY, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UKJames Cockram, John Bingham Laboratory, NIAB, Cambridge, UKPaul Nicholson, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UK.GODDARD, R.STEED, A.CHINOY, C.FERREIRA, J. R.SCHEEREN, P. L.MACIEL, J. L. N.CAIERAO, E.TORRES, G. A. M.CONSOLI, L.SANTANA, F. M.FERNANDES, J. M. C.SIMMONDS, J.UAUY, C.COCKRAM, J.NICHOLSON, P.2020-09-11T17:14:54Z2020-09-11T17:14:54Z2020-09-112020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBMC Plant Biology, v. 20, n. 398, Aug. 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124870https://doi.org/10.1186/s12870-020-02592-0enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2020-09-11T17:15:00Zoai:www.alice.cnptia.embrapa.br:doc/1124870Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542020-09-11T17:15falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542020-09-11T17:15Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena. |
title |
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena. |
spellingShingle |
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena. GODDARD, R. Wheat blast Seedling resistance Head resistance Single nucleotide polymorphism (SNP) genotyping Triticum Aestivum Magnaporthe oryzae Quantitative trait loci Genotyping |
title_short |
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena. |
title_full |
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena. |
title_fullStr |
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena. |
title_full_unstemmed |
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena. |
title_sort |
Dissecting the genetic basis of wheat blast resistance in the Brazilian wheat cultivar BR 18-Terena. |
author |
GODDARD, R. |
author_facet |
GODDARD, R. STEED, A. CHINOY, C. FERREIRA, J. R. SCHEEREN, P. L. MACIEL, J. L. N. CAIERAO, E. TORRES, G. A. M. CONSOLI, L. SANTANA, F. M. FERNANDES, J. M. C. SIMMONDS, J. UAUY, C. COCKRAM, J. NICHOLSON, P. |
author_role |
author |
author2 |
STEED, A. CHINOY, C. FERREIRA, J. R. SCHEEREN, P. L. MACIEL, J. L. N. CAIERAO, E. TORRES, G. A. M. CONSOLI, L. SANTANA, F. M. FERNANDES, J. M. C. SIMMONDS, J. UAUY, C. COCKRAM, J. NICHOLSON, P. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
RACHEL R. GODDARD, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UK ANDREW STEED, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UK CATHERINE CHINOY, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UK JÉSSICA ROSSET FERREIRA, Universidade de Passo Fundo, Passo Fundo, RS, Brazil PEDRO LUIZ SCHEEREN, CNPT JOAO LEODATO NUNES MACIEL, CNPT EDUARDO CAIERAO, CNPT GISELE ABIGAIL MONTAN TORRES, CNPT LUCIANO CONSOLI, CNPT FLAVIO MARTINS SANTANA, CNPT JOSE MAURICIO CUNHA FERNANDES, CNPT James Simmonds, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UK CRISTOBAL UAUY, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UK James Cockram, John Bingham Laboratory, NIAB, Cambridge, UK Paul Nicholson, Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich, UK. |
dc.contributor.author.fl_str_mv |
GODDARD, R. STEED, A. CHINOY, C. FERREIRA, J. R. SCHEEREN, P. L. MACIEL, J. L. N. CAIERAO, E. TORRES, G. A. M. CONSOLI, L. SANTANA, F. M. FERNANDES, J. M. C. SIMMONDS, J. UAUY, C. COCKRAM, J. NICHOLSON, P. |
dc.subject.por.fl_str_mv |
Wheat blast Seedling resistance Head resistance Single nucleotide polymorphism (SNP) genotyping Triticum Aestivum Magnaporthe oryzae Quantitative trait loci Genotyping |
topic |
Wheat blast Seedling resistance Head resistance Single nucleotide polymorphism (SNP) genotyping Triticum Aestivum Magnaporthe oryzae Quantitative trait loci Genotyping |
description |
Background: Wheat blast, caused by Magnaporthe oryzae Triticum (MoT) pathotype, is a global threat to wheat (Triticum aestivum L.) production. Few blast resistance (R) genes have been identified to date, therefore assessing potential sources of resistance in wheat is important. The Brazilian wheat cultivar BR 18-Terena is considered one of the best sources of resistance to blast and has been widely used in Brazilian breeding programmes, however the underlying genetics of this resistance are unknown. Results: BR 18-Terena was used as the common parent in the development of two recombinant inbred line (RIL) F6 populations with the Brazilian cultivars Anahuac 75 and BRS 179. Populations were phenotyped for resistance at the seedling and heading stage using the sequenced MoT isolate BR32, with transgressive segregation being observed. Genetic maps containing 1779 and 1318 markers, were produced for the Anahuac 75 × BR 18-Terena and BR 18- Terena × BRS 179 populations, respectively. Five quantitative trait loci (QTL) associated with seedling resistance, on chromosomes 2B, 4B (2 QTL), 5A and 6A, were identified, as were four QTL associated with heading stage resistance (1A, 2B, 4A and 5A). Seedling and heading stage QTL did not co-locate, despite a significant positive correlation between these traits, indicating that resistance at these developmental stages is likely to be controlled by different genes. BR 18-Terena provided the resistant allele for six QTL, at both developmental stages, with the largest phenotypic effect conferred by a QTL being 24.8% suggesting that BR 18-Terena possesses quantitative resistance. Haplotype analysis of 100 Brazilian wheat cultivars indicates that 11.0% of cultivars already possess a BR 18-Terenalike haplotype for more than one of the identified heading stage QTL. Conclusions: This study suggests that BR 18-Terena possesses quantitative resistance to wheat blast, with nine QTL associated with resistance at either the seedling or heading stage being detected. Wheat blast resistance is also largely tissue-specific. Identification of durable quantitative resistances which can be combined with race-specific R gene-mediated resistance is critical to effectively control wheat blast. Collectively, this work facilitates markerassisted selection to develop new varieties for cultivation in regions at risk from this emerging disease. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-11T17:14:54Z 2020-09-11T17:14:54Z 2020-09-11 2020 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BMC Plant Biology, v. 20, n. 398, Aug. 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124870 https://doi.org/10.1186/s12870-020-02592-0 |
identifier_str_mv |
BMC Plant Biology, v. 20, n. 398, Aug. 2020. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1124870 https://doi.org/10.1186/s12870-020-02592-0 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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