Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.

Detalhes bibliográficos
Autor(a) principal: CARVALHO, F. S.
Data de Publicação: 2011
Outros Autores: WENCESLAU, A. A., TEIXEIRA, M., ALBUQUERQUE, G. R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/908376
Resumo: Abstract - We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR. Individualized PCR with the phenol/chloroform DNA extraction protocol detected a larger number of Eimeria species. Molecular diagnosis was found to be practical and precise, and can be used for monitoring and epidemiological studies of Eimeria.
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spelling Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.PCRReação em cadeiaPolimeraseMultiplex PCRBroilersEimeriosisPrevalênciaFrangoEimeriaAbstract - We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR. Individualized PCR with the phenol/chloroform DNA extraction protocol detected a larger number of Eimeria species. Molecular diagnosis was found to be practical and precise, and can be used for monitoring and epidemiological studies of Eimeria.F.S. Carvalho, Universidade Estadual de Santa Cruz - Ilhéus, BA.; A.A. Wenceslau, Universidade Estadual de Santa Cruz - Ilhéus, BA; MARCEL TEIXEIRA, CNPC; G.R. Albuquerque, Universidade Estadual de Santa Cruz - Ilhéus, BA.CARVALHO, F. S.WENCESLAU, A. A.TEIXEIRA, M.ALBUQUERQUE, G. R.2018-01-26T23:36:59Z2018-01-26T23:36:59Z2011-12-0220112019-09-23T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 10, n. 2, p. 996-1005, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/90837610.4238/vol10-2gmr1043enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-01-26T23:37:06Zoai:www.alice.cnptia.embrapa.br:doc/908376Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-01-26T23:37:06falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-01-26T23:37:06Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
title Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
spellingShingle Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
CARVALHO, F. S.
PCR
Reação em cadeia
Polimerase
Multiplex PCR
Broilers
Eimeriosis
Prevalência
Frango
Eimeria
title_short Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
title_full Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
title_fullStr Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
title_full_unstemmed Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
title_sort Molecular diagnosis of Eimeria species affecting naturally infected Gallus gallus.
author CARVALHO, F. S.
author_facet CARVALHO, F. S.
WENCESLAU, A. A.
TEIXEIRA, M.
ALBUQUERQUE, G. R.
author_role author
author2 WENCESLAU, A. A.
TEIXEIRA, M.
ALBUQUERQUE, G. R.
author2_role author
author
author
dc.contributor.none.fl_str_mv F.S. Carvalho, Universidade Estadual de Santa Cruz - Ilhéus, BA.; A.A. Wenceslau, Universidade Estadual de Santa Cruz - Ilhéus, BA; MARCEL TEIXEIRA, CNPC; G.R. Albuquerque, Universidade Estadual de Santa Cruz - Ilhéus, BA.
dc.contributor.author.fl_str_mv CARVALHO, F. S.
WENCESLAU, A. A.
TEIXEIRA, M.
ALBUQUERQUE, G. R.
dc.subject.por.fl_str_mv PCR
Reação em cadeia
Polimerase
Multiplex PCR
Broilers
Eimeriosis
Prevalência
Frango
Eimeria
topic PCR
Reação em cadeia
Polimerase
Multiplex PCR
Broilers
Eimeriosis
Prevalência
Frango
Eimeria
description Abstract - We used PCR to test various protocols and define a technique for DNA extraction directly from chicken-shed stool samples for the identification of Eimeria species that parasitize birds. It was possible to extract and amplify DNA of seven Eimeria species from field stool samples, using both protocols tested; extractions made with phenol/chloroform protocols gave the best results. The primers were specific and sensitive, allowing amplification of samples containing as few as 20 oocysts, both in individual and in a multiplex PCR. Individualized PCR with the phenol/chloroform DNA extraction protocol detected a larger number of Eimeria species. Molecular diagnosis was found to be practical and precise, and can be used for monitoring and epidemiological studies of Eimeria.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-02
2011
2018-01-26T23:36:59Z
2018-01-26T23:36:59Z
2019-09-23T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Research, v. 10, n. 2, p. 996-1005, 2011.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/908376
10.4238/vol10-2gmr1043
identifier_str_mv Genetics and Molecular Research, v. 10, n. 2, p. 996-1005, 2011.
10.4238/vol10-2gmr1043
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/908376
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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