New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100557 |
Resumo: | The geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These plasmids are suitable to be used as CRM for Embrapa 5.1 GM common bean. They will be useful for survey of food labels for compliance with legislation about GMO content in Brazil and in other countries where GM common bean is not yet approved for commercialization. |
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spelling |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR.Certified reference materialEMB-PV051-1-eventFeijãoPhaseolus VulgarisOrganismo TransgênicoEngenharia GenéticaPlasmídeoBeansPlasmidsGenetically modified organismsQuantitative polymerase chain reactionThe geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These plasmids are suitable to be used as CRM for Embrapa 5.1 GM common bean. They will be useful for survey of food labels for compliance with legislation about GMO content in Brazil and in other countries where GM common bean is not yet approved for commercialization.GUSTAVO LUIZ VENTURELLI, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; KELLY JUSTIN DA SILVA, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; DIANA TREML, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; PAOLA BEATRIZ NAVAS, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; MARYELLA OSÓRIO VARGAS, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; JOANA LAURA BISCHOFF, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; JOSIAS CORREA DE FARIA, CNPAF; ANA CAROLINA MAISONNAVE ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC.VENTURELLI, G. L.SILVA, K. J. daTREML, D.NAVAS, P. B.VARGAS, M. O.BISCHOFF, J. L.FARIA, J. C. deARISI, A. C. M.2018-12-03T23:36:23Z2018-12-03T23:36:23Z2018-12-0320182019-01-22T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFood Bioscience, v. 26, p. 145-151, Dec. 2018.2212-4292http://www.alice.cnptia.embrapa.br/alice/handle/doc/110055710.1016/j.fbio.2018.10.007enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-12-03T23:36:28Zoai:www.alice.cnptia.embrapa.br:doc/1100557Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-12-03T23:36:28falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-12-03T23:36:28Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. |
title |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. |
spellingShingle |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. VENTURELLI, G. L. Certified reference material EMB-PV051-1-event Feijão Phaseolus Vulgaris Organismo Transgênico Engenharia Genética Plasmídeo Beans Plasmids Genetically modified organisms Quantitative polymerase chain reaction |
title_short |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. |
title_full |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. |
title_fullStr |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. |
title_full_unstemmed |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. |
title_sort |
New plasmid calibrators for geminivirus-resistant (EMB-PV051-1 event) common bean (Phaseolus vulgaris L.) quantitation using simplex and duplex qPCR. |
author |
VENTURELLI, G. L. |
author_facet |
VENTURELLI, G. L. SILVA, K. J. da TREML, D. NAVAS, P. B. VARGAS, M. O. BISCHOFF, J. L. FARIA, J. C. de ARISI, A. C. M. |
author_role |
author |
author2 |
SILVA, K. J. da TREML, D. NAVAS, P. B. VARGAS, M. O. BISCHOFF, J. L. FARIA, J. C. de ARISI, A. C. M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
GUSTAVO LUIZ VENTURELLI, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; KELLY JUSTIN DA SILVA, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; DIANA TREML, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; PAOLA BEATRIZ NAVAS, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; MARYELLA OSÓRIO VARGAS, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; JOANA LAURA BISCHOFF, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC; JOSIAS CORREA DE FARIA, CNPAF; ANA CAROLINA MAISONNAVE ARISI, UNIVERSIDADE FEDERAL DE SANTA CATARINA, Florianópolis-SC. |
dc.contributor.author.fl_str_mv |
VENTURELLI, G. L. SILVA, K. J. da TREML, D. NAVAS, P. B. VARGAS, M. O. BISCHOFF, J. L. FARIA, J. C. de ARISI, A. C. M. |
dc.subject.por.fl_str_mv |
Certified reference material EMB-PV051-1-event Feijão Phaseolus Vulgaris Organismo Transgênico Engenharia Genética Plasmídeo Beans Plasmids Genetically modified organisms Quantitative polymerase chain reaction |
topic |
Certified reference material EMB-PV051-1-event Feijão Phaseolus Vulgaris Organismo Transgênico Engenharia Genética Plasmídeo Beans Plasmids Genetically modified organisms Quantitative polymerase chain reaction |
description |
The geminivirus-resistant common bean, Embrapa 5.1, was the first commercial genetically modified (GM) event developed in Latin America by the Brazilian Agricultural Research Corp.. Therein novel standard reference plasmids were constructed for species-specific (pLEC) and event-specific (pFGM) qualitative and quantitative detection of Embrapa 5.1 GM common bean. To establish these plasmids as certified reference materials (CRM) for Embrapa 5.1 GM common bean, two DNA extraction protocols, simplex and duplex qPCR using two different operators (experimenters) were tested. The efficiency values ranged from 92% to 110% for the simplex and duplex reactions considering both operators. The limit of detection was enough to detect at least 0.1% GM content. These plasmids are suitable to be used as CRM for Embrapa 5.1 GM common bean. They will be useful for survey of food labels for compliance with legislation about GMO content in Brazil and in other countries where GM common bean is not yet approved for commercialization. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-03T23:36:23Z 2018-12-03T23:36:23Z 2018-12-03 2018 2019-01-22T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Food Bioscience, v. 26, p. 145-151, Dec. 2018. 2212-4292 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100557 10.1016/j.fbio.2018.10.007 |
identifier_str_mv |
Food Bioscience, v. 26, p. 145-151, Dec. 2018. 2212-4292 10.1016/j.fbio.2018.10.007 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100557 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503466234675200 |