Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.

Detalhes bibliográficos
Autor(a) principal: PAULA, C. M. P.
Data de Publicação: 2016
Outros Autores: FIGUEIREDO, K. G., SOUZA SOBRINHO, F. de, BENITES, F. R. G., DAVIDE, L. C., TECHIO, V. H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056860
https://doi.org/10.4238/gmr.15038737
Resumo: The genus Brachiaria contains species that have great economic importance in the Brazilian agricultural sector, as they enable cattle ranching on acid and poor soils with species that are resistant to spittlebugs and form crop-livestock-forest integration systems. The genus mainly consists of tetraploid (2n = 4x = 36) and apomictic species such as B. decumbens and B. brizantha. Sexuality is found in diploid species (2n = 2x = 18) such as B. ruziziensis. Interspecific hybridization between species of interest is possible by the artificial tetraploidization of B. ruziziensis and the subsequent hybridization with genotypes of B. brizantha and B. decumbens. Therefore, tetraploidized plants have to have normal meiosis or low rates of irregularities, as well as produce viable pollen grains. The objective of this study was to compare meiosis and pollen grain viability and morphology in artificially tetraploidized B. ruziziensis with that of descendants generated from crossing and selfing. The frequency of meiotic abnormalities ranged from 4.43 to 11%, and pollen viability ranged from 61 to 85%. Abnormalities were detected from prophase I to the tetrad stage with a variable frequency between the genotypes. The meiotic behavior of the artificially tetraploidized plants was little affected, and the pollen viability of the genotypes was high. Regarding pollen grain ultrastructure, there were no variations or morphological changes in the different genotypes. The genotypes have meiotic stability and high pollen viability, and can be incorporated into Brachiaria breeding programs.
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spelling Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.Pollen grainbreedingforagemeiosispolyploidyThe genus Brachiaria contains species that have great economic importance in the Brazilian agricultural sector, as they enable cattle ranching on acid and poor soils with species that are resistant to spittlebugs and form crop-livestock-forest integration systems. The genus mainly consists of tetraploid (2n = 4x = 36) and apomictic species such as B. decumbens and B. brizantha. Sexuality is found in diploid species (2n = 2x = 18) such as B. ruziziensis. Interspecific hybridization between species of interest is possible by the artificial tetraploidization of B. ruziziensis and the subsequent hybridization with genotypes of B. brizantha and B. decumbens. Therefore, tetraploidized plants have to have normal meiosis or low rates of irregularities, as well as produce viable pollen grains. The objective of this study was to compare meiosis and pollen grain viability and morphology in artificially tetraploidized B. ruziziensis with that of descendants generated from crossing and selfing. The frequency of meiotic abnormalities ranged from 4.43 to 11%, and pollen viability ranged from 61 to 85%. Abnormalities were detected from prophase I to the tetrad stage with a variable frequency between the genotypes. The meiotic behavior of the artificially tetraploidized plants was little affected, and the pollen viability of the genotypes was high. Regarding pollen grain ultrastructure, there were no variations or morphological changes in the different genotypes. The genotypes have meiotic stability and high pollen viability, and can be incorporated into Brachiaria breeding programs.C.M.P. Paula, UFLA; K. G. Figueiredo, UFLA; FAUSTO DE SOUZA SOBRINHO, CNPGL; FLAVIO RODRIGO GANDOLFI BENITES, CNPGL; L. C. Davide, UFLA; V. H. Techio, UFLA.PAULA, C. M. P.FIGUEIREDO, K. G.SOUZA SOBRINHO, F. deBENITES, F. R. G.DAVIDE, L. C.TECHIO, V. H.2016-11-23T11:11:11Z2016-11-23T11:11:11Z2016-11-2320162016-11-23T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 15, n. 3, gmr.15038737, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056860https://doi.org/10.4238/gmr.15038737enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:49:03Zoai:www.alice.cnptia.embrapa.br:doc/1056860Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:49:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:49:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
title Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
spellingShingle Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
PAULA, C. M. P.
Pollen grain
breeding
forage
meiosis
polyploidy
title_short Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
title_full Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
title_fullStr Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
title_full_unstemmed Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
title_sort Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
author PAULA, C. M. P.
author_facet PAULA, C. M. P.
FIGUEIREDO, K. G.
SOUZA SOBRINHO, F. de
BENITES, F. R. G.
DAVIDE, L. C.
TECHIO, V. H.
author_role author
author2 FIGUEIREDO, K. G.
SOUZA SOBRINHO, F. de
BENITES, F. R. G.
DAVIDE, L. C.
TECHIO, V. H.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv C.M.P. Paula, UFLA; K. G. Figueiredo, UFLA; FAUSTO DE SOUZA SOBRINHO, CNPGL; FLAVIO RODRIGO GANDOLFI BENITES, CNPGL; L. C. Davide, UFLA; V. H. Techio, UFLA.
dc.contributor.author.fl_str_mv PAULA, C. M. P.
FIGUEIREDO, K. G.
SOUZA SOBRINHO, F. de
BENITES, F. R. G.
DAVIDE, L. C.
TECHIO, V. H.
dc.subject.por.fl_str_mv Pollen grain
breeding
forage
meiosis
polyploidy
topic Pollen grain
breeding
forage
meiosis
polyploidy
description The genus Brachiaria contains species that have great economic importance in the Brazilian agricultural sector, as they enable cattle ranching on acid and poor soils with species that are resistant to spittlebugs and form crop-livestock-forest integration systems. The genus mainly consists of tetraploid (2n = 4x = 36) and apomictic species such as B. decumbens and B. brizantha. Sexuality is found in diploid species (2n = 2x = 18) such as B. ruziziensis. Interspecific hybridization between species of interest is possible by the artificial tetraploidization of B. ruziziensis and the subsequent hybridization with genotypes of B. brizantha and B. decumbens. Therefore, tetraploidized plants have to have normal meiosis or low rates of irregularities, as well as produce viable pollen grains. The objective of this study was to compare meiosis and pollen grain viability and morphology in artificially tetraploidized B. ruziziensis with that of descendants generated from crossing and selfing. The frequency of meiotic abnormalities ranged from 4.43 to 11%, and pollen viability ranged from 61 to 85%. Abnormalities were detected from prophase I to the tetrad stage with a variable frequency between the genotypes. The meiotic behavior of the artificially tetraploidized plants was little affected, and the pollen viability of the genotypes was high. Regarding pollen grain ultrastructure, there were no variations or morphological changes in the different genotypes. The genotypes have meiotic stability and high pollen viability, and can be incorporated into Brachiaria breeding programs.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-23T11:11:11Z
2016-11-23T11:11:11Z
2016-11-23
2016
2016-11-23T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Research, v. 15, n. 3, gmr.15038737, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056860
https://doi.org/10.4238/gmr.15038737
identifier_str_mv Genetics and Molecular Research, v. 15, n. 3, gmr.15038737, 2016.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056860
https://doi.org/10.4238/gmr.15038737
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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repository.mail.fl_str_mv cg-riaa@embrapa.br
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