Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056860 https://doi.org/10.4238/gmr.15038737 |
Resumo: | The genus Brachiaria contains species that have great economic importance in the Brazilian agricultural sector, as they enable cattle ranching on acid and poor soils with species that are resistant to spittlebugs and form crop-livestock-forest integration systems. The genus mainly consists of tetraploid (2n = 4x = 36) and apomictic species such as B. decumbens and B. brizantha. Sexuality is found in diploid species (2n = 2x = 18) such as B. ruziziensis. Interspecific hybridization between species of interest is possible by the artificial tetraploidization of B. ruziziensis and the subsequent hybridization with genotypes of B. brizantha and B. decumbens. Therefore, tetraploidized plants have to have normal meiosis or low rates of irregularities, as well as produce viable pollen grains. The objective of this study was to compare meiosis and pollen grain viability and morphology in artificially tetraploidized B. ruziziensis with that of descendants generated from crossing and selfing. The frequency of meiotic abnormalities ranged from 4.43 to 11%, and pollen viability ranged from 61 to 85%. Abnormalities were detected from prophase I to the tetrad stage with a variable frequency between the genotypes. The meiotic behavior of the artificially tetraploidized plants was little affected, and the pollen viability of the genotypes was high. Regarding pollen grain ultrastructure, there were no variations or morphological changes in the different genotypes. The genotypes have meiotic stability and high pollen viability, and can be incorporated into Brachiaria breeding programs. |
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Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs.Pollen grainbreedingforagemeiosispolyploidyThe genus Brachiaria contains species that have great economic importance in the Brazilian agricultural sector, as they enable cattle ranching on acid and poor soils with species that are resistant to spittlebugs and form crop-livestock-forest integration systems. The genus mainly consists of tetraploid (2n = 4x = 36) and apomictic species such as B. decumbens and B. brizantha. Sexuality is found in diploid species (2n = 2x = 18) such as B. ruziziensis. Interspecific hybridization between species of interest is possible by the artificial tetraploidization of B. ruziziensis and the subsequent hybridization with genotypes of B. brizantha and B. decumbens. Therefore, tetraploidized plants have to have normal meiosis or low rates of irregularities, as well as produce viable pollen grains. The objective of this study was to compare meiosis and pollen grain viability and morphology in artificially tetraploidized B. ruziziensis with that of descendants generated from crossing and selfing. The frequency of meiotic abnormalities ranged from 4.43 to 11%, and pollen viability ranged from 61 to 85%. Abnormalities were detected from prophase I to the tetrad stage with a variable frequency between the genotypes. The meiotic behavior of the artificially tetraploidized plants was little affected, and the pollen viability of the genotypes was high. Regarding pollen grain ultrastructure, there were no variations or morphological changes in the different genotypes. The genotypes have meiotic stability and high pollen viability, and can be incorporated into Brachiaria breeding programs.C.M.P. Paula, UFLA; K. G. Figueiredo, UFLA; FAUSTO DE SOUZA SOBRINHO, CNPGL; FLAVIO RODRIGO GANDOLFI BENITES, CNPGL; L. C. Davide, UFLA; V. H. Techio, UFLA.PAULA, C. M. P.FIGUEIREDO, K. G.SOUZA SOBRINHO, F. deBENITES, F. R. G.DAVIDE, L. C.TECHIO, V. H.2016-11-23T11:11:11Z2016-11-23T11:11:11Z2016-11-2320162016-11-23T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 15, n. 3, gmr.15038737, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056860https://doi.org/10.4238/gmr.15038737enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:49:03Zoai:www.alice.cnptia.embrapa.br:doc/1056860Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:49:03falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:49:03Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs. |
title |
Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs. |
spellingShingle |
Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs. PAULA, C. M. P. Pollen grain breeding forage meiosis polyploidy |
title_short |
Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs. |
title_full |
Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs. |
title_fullStr |
Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs. |
title_full_unstemmed |
Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs. |
title_sort |
Microsporogenesis analysis validates the use of artificially tetraploidized Brachiaria ruziziensis in breeding programs. |
author |
PAULA, C. M. P. |
author_facet |
PAULA, C. M. P. FIGUEIREDO, K. G. SOUZA SOBRINHO, F. de BENITES, F. R. G. DAVIDE, L. C. TECHIO, V. H. |
author_role |
author |
author2 |
FIGUEIREDO, K. G. SOUZA SOBRINHO, F. de BENITES, F. R. G. DAVIDE, L. C. TECHIO, V. H. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
C.M.P. Paula, UFLA; K. G. Figueiredo, UFLA; FAUSTO DE SOUZA SOBRINHO, CNPGL; FLAVIO RODRIGO GANDOLFI BENITES, CNPGL; L. C. Davide, UFLA; V. H. Techio, UFLA. |
dc.contributor.author.fl_str_mv |
PAULA, C. M. P. FIGUEIREDO, K. G. SOUZA SOBRINHO, F. de BENITES, F. R. G. DAVIDE, L. C. TECHIO, V. H. |
dc.subject.por.fl_str_mv |
Pollen grain breeding forage meiosis polyploidy |
topic |
Pollen grain breeding forage meiosis polyploidy |
description |
The genus Brachiaria contains species that have great economic importance in the Brazilian agricultural sector, as they enable cattle ranching on acid and poor soils with species that are resistant to spittlebugs and form crop-livestock-forest integration systems. The genus mainly consists of tetraploid (2n = 4x = 36) and apomictic species such as B. decumbens and B. brizantha. Sexuality is found in diploid species (2n = 2x = 18) such as B. ruziziensis. Interspecific hybridization between species of interest is possible by the artificial tetraploidization of B. ruziziensis and the subsequent hybridization with genotypes of B. brizantha and B. decumbens. Therefore, tetraploidized plants have to have normal meiosis or low rates of irregularities, as well as produce viable pollen grains. The objective of this study was to compare meiosis and pollen grain viability and morphology in artificially tetraploidized B. ruziziensis with that of descendants generated from crossing and selfing. The frequency of meiotic abnormalities ranged from 4.43 to 11%, and pollen viability ranged from 61 to 85%. Abnormalities were detected from prophase I to the tetrad stage with a variable frequency between the genotypes. The meiotic behavior of the artificially tetraploidized plants was little affected, and the pollen viability of the genotypes was high. Regarding pollen grain ultrastructure, there were no variations or morphological changes in the different genotypes. The genotypes have meiotic stability and high pollen viability, and can be incorporated into Brachiaria breeding programs. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-23T11:11:11Z 2016-11-23T11:11:11Z 2016-11-23 2016 2016-11-23T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 15, n. 3, gmr.15038737, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056860 https://doi.org/10.4238/gmr.15038737 |
identifier_str_mv |
Genetics and Molecular Research, v. 15, n. 3, gmr.15038737, 2016. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1056860 https://doi.org/10.4238/gmr.15038737 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503429018615808 |