Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae).
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138446 https://doi.org/10.3390/insects12040308 |
Resumo: | Abstract: A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose?response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility/mortality levels compared to males from the bisexual strain at doses < 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia. |
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Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae).LineageSouth american fruit flyAnastrepha FraterculusEsterilizaçãoMosca das FrutasIrradiationFruit fliesSterile insect techniqueOxygenAbstract: A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose?response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility/mortality levels compared to males from the bisexual strain at doses < 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia.PALOMA DELLA GIUSTINA, CENA-USPTHIAGO MASTRANGELO, Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and AgricultureSOHEL AHMAD, Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and AgricultureGABRIEL MOURA MASCARIN, CNPMACARLOS CACERES, Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture.DELLA GIUSTINA, P.MASTRANGELO, T.AHMAD, S.MASCARIN, G. M.CACERES, C.2021-12-28T14:01:17Z2021-12-28T14:01:17Z2021-12-282021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlep. 1-12.Insects, v. 12, n. 4, Article 308, 2021.2075-4450http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138446https://doi.org/10.3390/insects12040308enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-12-28T14:01:26Zoai:www.alice.cnptia.embrapa.br:doc/1138446Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-12-28T14:01:26falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-12-28T14:01:26Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae). |
title |
Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae). |
spellingShingle |
Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae). DELLA GIUSTINA, P. Lineage South american fruit fly Anastrepha Fraterculus Esterilização Mosca das Frutas Irradiation Fruit flies Sterile insect technique Oxygen |
title_short |
Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae). |
title_full |
Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae). |
title_fullStr |
Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae). |
title_full_unstemmed |
Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae). |
title_sort |
Determining the sterilization doses under hypoxia for the novel black pupae genetic sexing strain of Anastrepha fraterculus (Diptera, Tephritidae). |
author |
DELLA GIUSTINA, P. |
author_facet |
DELLA GIUSTINA, P. MASTRANGELO, T. AHMAD, S. MASCARIN, G. M. CACERES, C. |
author_role |
author |
author2 |
MASTRANGELO, T. AHMAD, S. MASCARIN, G. M. CACERES, C. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
PALOMA DELLA GIUSTINA, CENA-USP THIAGO MASTRANGELO, Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture SOHEL AHMAD, Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture GABRIEL MOURA MASCARIN, CNPMA CARLOS CACERES, Insect Pest Control Laboratory, Joint FAO/IAEA Centre of Nuclear Techniques in Food and Agriculture. |
dc.contributor.author.fl_str_mv |
DELLA GIUSTINA, P. MASTRANGELO, T. AHMAD, S. MASCARIN, G. M. CACERES, C. |
dc.subject.por.fl_str_mv |
Lineage South american fruit fly Anastrepha Fraterculus Esterilização Mosca das Frutas Irradiation Fruit flies Sterile insect technique Oxygen |
topic |
Lineage South american fruit fly Anastrepha Fraterculus Esterilização Mosca das Frutas Irradiation Fruit flies Sterile insect technique Oxygen |
description |
Abstract: A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose?response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility/mortality levels compared to males from the bisexual strain at doses < 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-28T14:01:17Z 2021-12-28T14:01:17Z 2021-12-28 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Insects, v. 12, n. 4, Article 308, 2021. 2075-4450 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138446 https://doi.org/10.3390/insects12040308 |
identifier_str_mv |
Insects, v. 12, n. 4, Article 308, 2021. 2075-4450 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1138446 https://doi.org/10.3390/insects12040308 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
p. 1-12. |
dc.source.none.fl_str_mv |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
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EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503515685519360 |