Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.

Detalhes bibliográficos
Autor(a) principal: SANTOS, M. R. A. dos
Data de Publicação: 2017
Outros Autores: AGUIAR, C. B., MENDES, A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950
Resumo: Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day.
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spelling Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.CallogenesisGrowlh curvesecondary metabolitesChili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day.MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia.SANTOS, M. R. A. dosAGUIAR, C. B.MENDES, A. M.2017-11-28T23:30:36Z2017-11-28T23:30:36Z2017-11-2820172017-11-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInternational Journal of Development Research, v. 7, n. 6, p.13062-13066, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-11-28T23:30:44Zoai:www.alice.cnptia.embrapa.br:doc/1080950Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-11-28T23:30:44falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-11-28T23:30:44Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
title Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
spellingShingle Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
SANTOS, M. R. A. dos
Callogenesis
Growlh curve
secondary metabolites
title_short Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
title_full Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
title_fullStr Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
title_full_unstemmed Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
title_sort Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
author SANTOS, M. R. A. dos
author_facet SANTOS, M. R. A. dos
AGUIAR, C. B.
MENDES, A. M.
author_role author
author2 AGUIAR, C. B.
MENDES, A. M.
author2_role author
author
dc.contributor.none.fl_str_mv MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia.
dc.contributor.author.fl_str_mv SANTOS, M. R. A. dos
AGUIAR, C. B.
MENDES, A. M.
dc.subject.por.fl_str_mv Callogenesis
Growlh curve
secondary metabolites
topic Callogenesis
Growlh curve
secondary metabolites
description Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-28T23:30:36Z
2017-11-28T23:30:36Z
2017-11-28
2017
2017-11-29T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv International Journal of Development Research, v. 7, n. 6, p.13062-13066, 2017.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950
identifier_str_mv International Journal of Development Research, v. 7, n. 6, p.13062-13066, 2017.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950
dc.language.iso.fl_str_mv eng
language eng
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instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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