Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950 |
Resumo: | Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day. |
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Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama.CallogenesisGrowlh curvesecondary metabolitesChili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day.MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia.SANTOS, M. R. A. dosAGUIAR, C. B.MENDES, A. M.2017-11-28T23:30:36Z2017-11-28T23:30:36Z2017-11-2820172017-11-29T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInternational Journal of Development Research, v. 7, n. 6, p.13062-13066, 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-11-28T23:30:44Zoai:www.alice.cnptia.embrapa.br:doc/1080950Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-11-28T23:30:44falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-11-28T23:30:44Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. |
title |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. |
spellingShingle |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. SANTOS, M. R. A. dos Callogenesis Growlh curve secondary metabolites |
title_short |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. |
title_full |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. |
title_fullStr |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. |
title_full_unstemmed |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. |
title_sort |
Dedifferentiation and growth of friable calluses from stems of Capsicum chinense cv. Airetama. |
author |
SANTOS, M. R. A. dos |
author_facet |
SANTOS, M. R. A. dos AGUIAR, C. B. MENDES, A. M. |
author_role |
author |
author2 |
AGUIAR, C. B. MENDES, A. M. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-Rondonia. |
dc.contributor.author.fl_str_mv |
SANTOS, M. R. A. dos AGUIAR, C. B. MENDES, A. M. |
dc.subject.por.fl_str_mv |
Callogenesis Growlh curve secondary metabolites |
topic |
Callogenesis Growlh curve secondary metabolites |
description |
Chili peppers belong to the genus Capsicum and have been studied for their bioactivity, provided by secondary metabolites, which can be used for medicinal and agricultural purposes. Some Capsicum species have been submitted to in vitro cultivation systems of cell suspensions, which have been largely reported as safe and standardized methods of production of secondary compounds. The objective of this study was to determine a protocol for the establishment of cell suspensions from stems of the cultivar C. chinense cv. Airetama. For this, it was necessary to determine the combinations and concentrations of growth regulators for callus induction and to study the growth pattern of the calluses with a focus on the deceleration phase. In this phase the callus cells must be subcultured in a liquid medium in order to establish cell suspension cultivations. Stem explants were inoculated into media supplemented with 2,4-D and BA. The proliferation of callus cells and their growth pattern were evaluated. The highest callus cell proliferation occurred with the combination of 18.10 µM 2,4-D + 2.22 µM BA. The calluses were friable and whitish and their growth pattern followed a sigmoid shape. The deceleration phase started on the 42nd day. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-28T23:30:36Z 2017-11-28T23:30:36Z 2017-11-28 2017 2017-11-29T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal of Development Research, v. 7, n. 6, p.13062-13066, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950 |
identifier_str_mv |
International Journal of Development Research, v. 7, n. 6, p.13062-13066, 2017. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1080950 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503445544173568 |