Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency.
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097743 |
Resumo: | Phosphate (Pi) unavailability is a growth-limiting factor for plants. Under Pi-limited conditions, plants activate molecular mechanisms for better acquisition and utilization of this nutrient. In maize, changes in the expression pattern of several Pi starvation-induced genes, including the A1 coding for dihydroflavonol 4-reductase (DFR) involved in anthocyanin biosynthesis, were identified through microarray analysis. In order to elucidate the molecular determinants with a potential role in P use efficiency, we carried out a study on gene expression analysis of the A1 phosphate responsive gene by northern blot analysis of total RNA from maize genotypes contrasting for Pi efficiency. Two Pi-efficient (L-03 and L-161-1) and five inefficient (L-11, L-16, L-22, L-53, and L-5046) genotypes of maize were grown for 15 days in hydroponic culture in the presence (250 ,uM Pi) or absence (0 ,uM Pi) of phosphate. All genotypes showed an increase in anthocyanin accumulation in roots in the absence of Pi (0 ,uM Pi). The Pi-efficient genotype L-36 and the Pi-inefficient genotypes L-16, L22, and L-5046 showed the highest levels of anthocyanin accumulation. The A1 gene exhibits temporal and spatial expression patterns associated with Pi deficiency. Although there were differences in the expression profile of Pi starvation induced genes, no consistent expression patterns could be associated with either Pi-efficient or Pi-inefficient genotypes. It appears that Pi efficiency in tropical maize is a complex trait mediated by a coordinated action of genes that are either induced or suppressed in response to Pi-deficiency. |
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Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency.Estresse abiócoExpressão genéticaSolo ÁcidoAntocianinaPhosphate (Pi) unavailability is a growth-limiting factor for plants. Under Pi-limited conditions, plants activate molecular mechanisms for better acquisition and utilization of this nutrient. In maize, changes in the expression pattern of several Pi starvation-induced genes, including the A1 coding for dihydroflavonol 4-reductase (DFR) involved in anthocyanin biosynthesis, were identified through microarray analysis. In order to elucidate the molecular determinants with a potential role in P use efficiency, we carried out a study on gene expression analysis of the A1 phosphate responsive gene by northern blot analysis of total RNA from maize genotypes contrasting for Pi efficiency. Two Pi-efficient (L-03 and L-161-1) and five inefficient (L-11, L-16, L-22, L-53, and L-5046) genotypes of maize were grown for 15 days in hydroponic culture in the presence (250 ,uM Pi) or absence (0 ,uM Pi) of phosphate. All genotypes showed an increase in anthocyanin accumulation in roots in the absence of Pi (0 ,uM Pi). The Pi-efficient genotype L-36 and the Pi-inefficient genotypes L-16, L22, and L-5046 showed the highest levels of anthocyanin accumulation. The A1 gene exhibits temporal and spatial expression patterns associated with Pi deficiency. Although there were differences in the expression profile of Pi starvation induced genes, no consistent expression patterns could be associated with either Pi-efficient or Pi-inefficient genotypes. It appears that Pi efficiency in tropical maize is a complex trait mediated by a coordinated action of genes that are either induced or suppressed in response to Pi-deficiency.Article: gmr18036.MARIA JOSE VILACA DE VASCONCELOS, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; Purdue University.VASCONCELOS, M. J. V. deFIGUEIREDO, J. E. F.RAGHOTHAMA, K. G.2018-10-19T00:49:28Z2018-10-19T00:49:28Z2018-10-1820182018-10-19T00:49:28Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, Ribeirão Preto, v. 17, n. 4, p. 1-9, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/109774310.4238/gmr18036enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-10-19T00:49:34Zoai:www.alice.cnptia.embrapa.br:doc/1097743Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-10-19T00:49:34falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-10-19T00:49:34Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. |
title |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. |
spellingShingle |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. VASCONCELOS, M. J. V. de Estresse abióco Expressão genética Solo Ácido Antocianina |
title_short |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. |
title_full |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. |
title_fullStr |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. |
title_full_unstemmed |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. |
title_sort |
Expression analysis of anthocyanin gene induced under phosphorus starvation in maize genotypes with contrasting phosphorus use efficiency. |
author |
VASCONCELOS, M. J. V. de |
author_facet |
VASCONCELOS, M. J. V. de FIGUEIREDO, J. E. F. RAGHOTHAMA, K. G. |
author_role |
author |
author2 |
FIGUEIREDO, J. E. F. RAGHOTHAMA, K. G. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; Purdue University. |
dc.contributor.author.fl_str_mv |
VASCONCELOS, M. J. V. de FIGUEIREDO, J. E. F. RAGHOTHAMA, K. G. |
dc.subject.por.fl_str_mv |
Estresse abióco Expressão genética Solo Ácido Antocianina |
topic |
Estresse abióco Expressão genética Solo Ácido Antocianina |
description |
Phosphate (Pi) unavailability is a growth-limiting factor for plants. Under Pi-limited conditions, plants activate molecular mechanisms for better acquisition and utilization of this nutrient. In maize, changes in the expression pattern of several Pi starvation-induced genes, including the A1 coding for dihydroflavonol 4-reductase (DFR) involved in anthocyanin biosynthesis, were identified through microarray analysis. In order to elucidate the molecular determinants with a potential role in P use efficiency, we carried out a study on gene expression analysis of the A1 phosphate responsive gene by northern blot analysis of total RNA from maize genotypes contrasting for Pi efficiency. Two Pi-efficient (L-03 and L-161-1) and five inefficient (L-11, L-16, L-22, L-53, and L-5046) genotypes of maize were grown for 15 days in hydroponic culture in the presence (250 ,uM Pi) or absence (0 ,uM Pi) of phosphate. All genotypes showed an increase in anthocyanin accumulation in roots in the absence of Pi (0 ,uM Pi). The Pi-efficient genotype L-36 and the Pi-inefficient genotypes L-16, L22, and L-5046 showed the highest levels of anthocyanin accumulation. The A1 gene exhibits temporal and spatial expression patterns associated with Pi deficiency. Although there were differences in the expression profile of Pi starvation induced genes, no consistent expression patterns could be associated with either Pi-efficient or Pi-inefficient genotypes. It appears that Pi efficiency in tropical maize is a complex trait mediated by a coordinated action of genes that are either induced or suppressed in response to Pi-deficiency. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-10-19T00:49:28Z 2018-10-19T00:49:28Z 2018-10-18 2018 2018-10-19T00:49:28Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v. 17, n. 4, p. 1-9, 2018. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097743 10.4238/gmr18036 |
identifier_str_mv |
Genetics and Molecular Research, Ribeirão Preto, v. 17, n. 4, p. 1-9, 2018. 10.4238/gmr18036 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1097743 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503464103968768 |