Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781 https://doi.org/10.1016/j.anireprosci.2023.107278 |
Resumo: | This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes |
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Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.FoliculogêneseFolículos primordiaisOócitoHormônios sexuaisOvelhaÁgua PotávelDrinking waterOocytesSex hormonesSheepThis study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewesVANÚZIA GONÇALVES MENEZES, UNIVASFALANE PAINS OLIVEIRA DO MONTE, UNIVASFRICÁSSIO DE SOUSA BARBERINO, UNIVASFBRUNA BORTOLONI GOUVEIA, UNIVASFTHAE LANE BARBOSA GAMA LINS, UNIVASFTAÍS JOBARD SILVA E MACEDO, UNIVASFJOÃOZITO LIANDRO DE OLIVEIRA JÚNIOR, UNIVASFCLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste Pernambucano, Garanhuns, PEFLEMING SENA CAMPOS, Universidade Federal do Maranh ̃ao - UFMA, Chapadina, MAEMANUEL FELIPE DE OLIVEIRA FILHO, UFRPEANDRÉ MARIANO BATISTA, UFRPEMARIO ADRIANO ÁVILA QUEIROZ, UNIVASFGHERMAN GARCIA LEAL DE ARAUJO, CPATSAMARIA HELENA TAVARES DE MATOS, UNIVASFAUREA WISCHRA, UFRPE.MENEZES, V. G.MONTE, A. P. O.BARBERINO, R. de S.GOUVEIA, B. B.LINS, T. L. B.MACEDO, T. J. S. eOLIVEIRA JÚNIOR, J. L. deARAÚJO, C. de A.CAMPOS, F. S.OLIVEIRA FILHO, E. F. deBATISTA, A. M.QUEIROZ, M. A. A.ARAUJO, G. G. L. deMATOS, M. H. T. deWISCHRA, A.2023-09-19T17:24:21Z2023-09-19T17:24:21Z2023-09-192023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnimal Reproduction Science, v. 255, 107278, Jun. 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781https://doi.org/10.1016/j.anireprosci.2023.107278enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-19T17:24:21Zoai:www.alice.cnptia.embrapa.br:doc/1156781Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-19T17:24:21falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-19T17:24:21Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
title |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
spellingShingle |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. MENEZES, V. G. Foliculogênese Folículos primordiais Oócito Hormônios sexuais Ovelha Água Potável Drinking water Oocytes Sex hormones Sheep |
title_short |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
title_full |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
title_fullStr |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
title_full_unstemmed |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
title_sort |
Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles. |
author |
MENEZES, V. G. |
author_facet |
MENEZES, V. G. MONTE, A. P. O. BARBERINO, R. de S. GOUVEIA, B. B. LINS, T. L. B. MACEDO, T. J. S. e OLIVEIRA JÚNIOR, J. L. de ARAÚJO, C. de A. CAMPOS, F. S. OLIVEIRA FILHO, E. F. de BATISTA, A. M. QUEIROZ, M. A. A. ARAUJO, G. G. L. de MATOS, M. H. T. de WISCHRA, A. |
author_role |
author |
author2 |
MONTE, A. P. O. BARBERINO, R. de S. GOUVEIA, B. B. LINS, T. L. B. MACEDO, T. J. S. e OLIVEIRA JÚNIOR, J. L. de ARAÚJO, C. de A. CAMPOS, F. S. OLIVEIRA FILHO, E. F. de BATISTA, A. M. QUEIROZ, M. A. A. ARAUJO, G. G. L. de MATOS, M. H. T. de WISCHRA, A. |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
VANÚZIA GONÇALVES MENEZES, UNIVASF ALANE PAINS OLIVEIRA DO MONTE, UNIVASF RICÁSSIO DE SOUSA BARBERINO, UNIVASF BRUNA BORTOLONI GOUVEIA, UNIVASF THAE LANE BARBOSA GAMA LINS, UNIVASF TAÍS JOBARD SILVA E MACEDO, UNIVASF JOÃOZITO LIANDRO DE OLIVEIRA JÚNIOR, UNIVASF CLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste Pernambucano, Garanhuns, PE FLEMING SENA CAMPOS, Universidade Federal do Maranh ̃ao - UFMA, Chapadina, MA EMANUEL FELIPE DE OLIVEIRA FILHO, UFRPE ANDRÉ MARIANO BATISTA, UFRPE MARIO ADRIANO ÁVILA QUEIROZ, UNIVASF GHERMAN GARCIA LEAL DE ARAUJO, CPATSA MARIA HELENA TAVARES DE MATOS, UNIVASF AUREA WISCHRA, UFRPE. |
dc.contributor.author.fl_str_mv |
MENEZES, V. G. MONTE, A. P. O. BARBERINO, R. de S. GOUVEIA, B. B. LINS, T. L. B. MACEDO, T. J. S. e OLIVEIRA JÚNIOR, J. L. de ARAÚJO, C. de A. CAMPOS, F. S. OLIVEIRA FILHO, E. F. de BATISTA, A. M. QUEIROZ, M. A. A. ARAUJO, G. G. L. de MATOS, M. H. T. de WISCHRA, A. |
dc.subject.por.fl_str_mv |
Foliculogênese Folículos primordiais Oócito Hormônios sexuais Ovelha Água Potável Drinking water Oocytes Sex hormones Sheep |
topic |
Foliculogênese Folículos primordiais Oócito Hormônios sexuais Ovelha Água Potável Drinking water Oocytes Sex hormones Sheep |
description |
This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (α-MEM+) or α-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in α-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-09-19T17:24:21Z 2023-09-19T17:24:21Z 2023-09-19 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Animal Reproduction Science, v. 255, 107278, Jun. 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781 https://doi.org/10.1016/j.anireprosci.2023.107278 |
identifier_str_mv |
Animal Reproduction Science, v. 255, 107278, Jun. 2023. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781 https://doi.org/10.1016/j.anireprosci.2023.107278 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503549762142208 |