Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.

Detalhes bibliográficos
Autor(a) principal: MENEZES, V. G.
Data de Publicação: 2023
Outros Autores: MONTE, A. P. O., BARBERINO, R. de S., GOUVEIA, B. B., LINS, T. L. B., MACEDO, T. J. S. e, OLIVEIRA JÚNIOR, J. L. de, ARAÚJO, C. de A., CAMPOS, F. S., OLIVEIRA FILHO, E. F. de, BATISTA, A. M., QUEIROZ, M. A. A., ARAUJO, G. G. L. de, MATOS, M. H. T. de, WISCHRA, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781
https://doi.org/10.1016/j.anireprosci.2023.107278
Resumo: This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (&#945;-MEM+) or &#945;-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in &#945;-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes
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spelling Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.FoliculogêneseFolículos primordiaisOócitoHormônios sexuaisOvelhaÁgua PotávelDrinking waterOocytesSex hormonesSheepThis study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (&#945;-MEM+) or &#945;-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in &#945;-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewesVANÚZIA GONÇALVES MENEZES, UNIVASFALANE PAINS OLIVEIRA DO MONTE, UNIVASFRICÁSSIO DE SOUSA BARBERINO, UNIVASFBRUNA BORTOLONI GOUVEIA, UNIVASFTHAE LANE BARBOSA GAMA LINS, UNIVASFTAÍS JOBARD SILVA E MACEDO, UNIVASFJOÃOZITO LIANDRO DE OLIVEIRA JÚNIOR, UNIVASFCLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste Pernambucano, Garanhuns, PEFLEMING SENA CAMPOS, Universidade Federal do Maranh ̃ao - UFMA, Chapadina, MAEMANUEL FELIPE DE OLIVEIRA FILHO, UFRPEANDRÉ MARIANO BATISTA, UFRPEMARIO ADRIANO ÁVILA QUEIROZ, UNIVASFGHERMAN GARCIA LEAL DE ARAUJO, CPATSAMARIA HELENA TAVARES DE MATOS, UNIVASFAUREA WISCHRA, UFRPE.MENEZES, V. G.MONTE, A. P. O.BARBERINO, R. de S.GOUVEIA, B. B.LINS, T. L. B.MACEDO, T. J. S. eOLIVEIRA JÚNIOR, J. L. deARAÚJO, C. de A.CAMPOS, F. S.OLIVEIRA FILHO, E. F. deBATISTA, A. M.QUEIROZ, M. A. A.ARAUJO, G. G. L. deMATOS, M. H. T. deWISCHRA, A.2023-09-19T17:24:21Z2023-09-19T17:24:21Z2023-09-192023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnimal Reproduction Science, v. 255, 107278, Jun. 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781https://doi.org/10.1016/j.anireprosci.2023.107278enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-09-19T17:24:21Zoai:www.alice.cnptia.embrapa.br:doc/1156781Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-09-19T17:24:21falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-09-19T17:24:21Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
title Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
spellingShingle Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
MENEZES, V. G.
Foliculogênese
Folículos primordiais
Oócito
Hormônios sexuais
Ovelha
Água Potável
Drinking water
Oocytes
Sex hormones
Sheep
title_short Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
title_full Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
title_fullStr Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
title_full_unstemmed Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
title_sort Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
author MENEZES, V. G.
author_facet MENEZES, V. G.
MONTE, A. P. O.
BARBERINO, R. de S.
GOUVEIA, B. B.
LINS, T. L. B.
MACEDO, T. J. S. e
OLIVEIRA JÚNIOR, J. L. de
ARAÚJO, C. de A.
CAMPOS, F. S.
OLIVEIRA FILHO, E. F. de
BATISTA, A. M.
QUEIROZ, M. A. A.
ARAUJO, G. G. L. de
MATOS, M. H. T. de
WISCHRA, A.
author_role author
author2 MONTE, A. P. O.
BARBERINO, R. de S.
GOUVEIA, B. B.
LINS, T. L. B.
MACEDO, T. J. S. e
OLIVEIRA JÚNIOR, J. L. de
ARAÚJO, C. de A.
CAMPOS, F. S.
OLIVEIRA FILHO, E. F. de
BATISTA, A. M.
QUEIROZ, M. A. A.
ARAUJO, G. G. L. de
MATOS, M. H. T. de
WISCHRA, A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv VANÚZIA GONÇALVES MENEZES, UNIVASF
ALANE PAINS OLIVEIRA DO MONTE, UNIVASF
RICÁSSIO DE SOUSA BARBERINO, UNIVASF
BRUNA BORTOLONI GOUVEIA, UNIVASF
THAE LANE BARBOSA GAMA LINS, UNIVASF
TAÍS JOBARD SILVA E MACEDO, UNIVASF
JOÃOZITO LIANDRO DE OLIVEIRA JÚNIOR, UNIVASF
CLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste Pernambucano, Garanhuns, PE
FLEMING SENA CAMPOS, Universidade Federal do Maranh ̃ao - UFMA, Chapadina, MA
EMANUEL FELIPE DE OLIVEIRA FILHO, UFRPE
ANDRÉ MARIANO BATISTA, UFRPE
MARIO ADRIANO ÁVILA QUEIROZ, UNIVASF
GHERMAN GARCIA LEAL DE ARAUJO, CPATSA
MARIA HELENA TAVARES DE MATOS, UNIVASF
AUREA WISCHRA, UFRPE.
dc.contributor.author.fl_str_mv MENEZES, V. G.
MONTE, A. P. O.
BARBERINO, R. de S.
GOUVEIA, B. B.
LINS, T. L. B.
MACEDO, T. J. S. e
OLIVEIRA JÚNIOR, J. L. de
ARAÚJO, C. de A.
CAMPOS, F. S.
OLIVEIRA FILHO, E. F. de
BATISTA, A. M.
QUEIROZ, M. A. A.
ARAUJO, G. G. L. de
MATOS, M. H. T. de
WISCHRA, A.
dc.subject.por.fl_str_mv Foliculogênese
Folículos primordiais
Oócito
Hormônios sexuais
Ovelha
Água Potável
Drinking water
Oocytes
Sex hormones
Sheep
topic Foliculogênese
Folículos primordiais
Oócito
Hormônios sexuais
Ovelha
Água Potável
Drinking water
Oocytes
Sex hormones
Sheep
description This study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (&#945;-MEM+) or &#945;-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in &#945;-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes
publishDate 2023
dc.date.none.fl_str_mv 2023-09-19T17:24:21Z
2023-09-19T17:24:21Z
2023-09-19
2023
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Animal Reproduction Science, v. 255, 107278, Jun. 2023.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781
https://doi.org/10.1016/j.anireprosci.2023.107278
identifier_str_mv Animal Reproduction Science, v. 255, 107278, Jun. 2023.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781
https://doi.org/10.1016/j.anireprosci.2023.107278
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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