On the search for markers of tick resistance in bovines.

Detalhes bibliográficos
Autor(a) principal: REGITANO, L. C. A.
Data de Publicação: 2008
Outros Autores: IBELLI, A. M. G., GASPARIN, G., MIYATA, M., AZEVEDO, A. L. S., COUTINHO, L. L., TEODORO, R. L., MACHADO, M. A., SILVA, M. V. G. B., NAKATA, L. C., ZAROS, L. G., SONSTEGARD, T. S., SILVA, A. M., ALENCAR, M. M., OLIVEIRA, M. C. S.
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/596319
Resumo: Genetic differences in susceptibility to tick (Riphicephalus (Boophilus) microplus) are considerable in bovine. In general, Bos taurus is more susceptible to ticks than Bos indicus. On the search for the molecular basis of tick resistance we used mapping, association and gene expression approaches. A B. taurus x B. indicus F2 population was developed by Embrapa and 300 individuals were measured for parasite load and genotype for microsatellite markers covering chromosomes 5, 7 and 14. A suggestive chromosome-wide QTL for tick load (P < 0.05) was mapped on chromosome 5 and two suggestive (P < 0.10) QTL on chromosome 7 and 14. In the candidate gene approach, females from the genetic groups Nelore (NE -184), Canchim x Nelore (CN -153), Aberdeen Angus x Nelore (AN -123) and Simmental x Nelore (SN -120) were evaluated under natural infestation in seven to ten tick Counts. Microsatellite markers close to the genes for interleukin 2 (IL2), interleukin 4 (IL4) and interferongamma (lFNG) were analysed. Tick counts were associated to the marker for interleukin 4 (P < 0.05) in NE, CN and AN genetic groups. We also investigated if there were differences in cytokine mRNA of naive versus infested Nelore as well as of resistant versus susceptible cows from NE, CN and AN genetic groups. Relative quantification of cytokines from infested compared to naives showed down regulation of IL2. When resistant cows from the from genetic groups were. compared to susceptible animals, IL8 was down regulated but no differences were found for IL4 expression.
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spelling On the search for markers of tick resistance in bovines.Marcadores genéticosCarrapatoResistênciaGenetic differences in susceptibility to tick (Riphicephalus (Boophilus) microplus) are considerable in bovine. In general, Bos taurus is more susceptible to ticks than Bos indicus. On the search for the molecular basis of tick resistance we used mapping, association and gene expression approaches. A B. taurus x B. indicus F2 population was developed by Embrapa and 300 individuals were measured for parasite load and genotype for microsatellite markers covering chromosomes 5, 7 and 14. A suggestive chromosome-wide QTL for tick load (P < 0.05) was mapped on chromosome 5 and two suggestive (P < 0.10) QTL on chromosome 7 and 14. In the candidate gene approach, females from the genetic groups Nelore (NE -184), Canchim x Nelore (CN -153), Aberdeen Angus x Nelore (AN -123) and Simmental x Nelore (SN -120) were evaluated under natural infestation in seven to ten tick Counts. Microsatellite markers close to the genes for interleukin 2 (IL2), interleukin 4 (IL4) and interferongamma (lFNG) were analysed. Tick counts were associated to the marker for interleukin 4 (P < 0.05) in NE, CN and AN genetic groups. We also investigated if there were differences in cytokine mRNA of naive versus infested Nelore as well as of resistant versus susceptible cows from NE, CN and AN genetic groups. Relative quantification of cytokines from infested compared to naives showed down regulation of IL2. When resistant cows from the from genetic groups were. compared to susceptible animals, IL8 was down regulated but no differences were found for IL4 expression.Luciana Correia de Almeida Regitano - Embrapa Pecuária Sudeste; A.G. Ibelli - UFSCar; Gustavo Gasparin - UFSCar; Yukino Miyata - UFSCar; A. L. S. Azevedo, UFV; Luiz L. Coutinho - ESALQ/USP; Roberto Luiz Teodoro, Embrapa Gado de Leite; Marco Antonio Machado - Embrapa Gado de Leite; Marcus Vinicius G.B. Silva, Embrapa Gado de Leite; L. C. Nakata, UF São Carlos; L. G. Zaros, Esalq; Tad Stewart Sonstegard, USDA/ARS; A. M. Silva, UF São Carlos; Mauricio de Mello Alencar, Embrapa Pecuária Sudeste; M.C.S. Oliveira, Embrapa Pecuária Sudeste.REGITANO, L. C. A.IBELLI, A. M. G.GASPARIN, G.MIYATA, M.AZEVEDO, A. L. S.COUTINHO, L. L.TEODORO, R. L.MACHADO, M. A.SILVA, M. V. G. B.NAKATA, L. C.ZAROS, L. G.SONSTEGARD, T. S.SILVA, A. M.ALENCAR, M. M.OLIVEIRA, M. C. S.2024-06-17T12:55:14Z2024-06-17T12:55:14Z2009-02-192008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPartIn: PINARD, M.; GAY, C.; PASTORET, P.; DODET, B. (Ed.). Animal genomics for animal healt. Basel, Karger: Dev Biol, 2008. p. 225-230.http://www.alice.cnptia.embrapa.br/alice/handle/doc/596319enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-06-17T12:55:14Zoai:www.alice.cnptia.embrapa.br:doc/596319Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-06-17T12:55:14Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv On the search for markers of tick resistance in bovines.
title On the search for markers of tick resistance in bovines.
spellingShingle On the search for markers of tick resistance in bovines.
REGITANO, L. C. A.
Marcadores genéticos
Carrapato
Resistência
title_short On the search for markers of tick resistance in bovines.
title_full On the search for markers of tick resistance in bovines.
title_fullStr On the search for markers of tick resistance in bovines.
title_full_unstemmed On the search for markers of tick resistance in bovines.
title_sort On the search for markers of tick resistance in bovines.
author REGITANO, L. C. A.
author_facet REGITANO, L. C. A.
IBELLI, A. M. G.
GASPARIN, G.
MIYATA, M.
AZEVEDO, A. L. S.
COUTINHO, L. L.
TEODORO, R. L.
MACHADO, M. A.
SILVA, M. V. G. B.
NAKATA, L. C.
ZAROS, L. G.
SONSTEGARD, T. S.
SILVA, A. M.
ALENCAR, M. M.
OLIVEIRA, M. C. S.
author_role author
author2 IBELLI, A. M. G.
GASPARIN, G.
MIYATA, M.
AZEVEDO, A. L. S.
COUTINHO, L. L.
TEODORO, R. L.
MACHADO, M. A.
SILVA, M. V. G. B.
NAKATA, L. C.
ZAROS, L. G.
SONSTEGARD, T. S.
SILVA, A. M.
ALENCAR, M. M.
OLIVEIRA, M. C. S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Luciana Correia de Almeida Regitano - Embrapa Pecuária Sudeste; A.G. Ibelli - UFSCar; Gustavo Gasparin - UFSCar; Yukino Miyata - UFSCar; A. L. S. Azevedo, UFV; Luiz L. Coutinho - ESALQ/USP; Roberto Luiz Teodoro, Embrapa Gado de Leite; Marco Antonio Machado - Embrapa Gado de Leite; Marcus Vinicius G.B. Silva, Embrapa Gado de Leite; L. C. Nakata, UF São Carlos; L. G. Zaros, Esalq; Tad Stewart Sonstegard, USDA/ARS; A. M. Silva, UF São Carlos; Mauricio de Mello Alencar, Embrapa Pecuária Sudeste; M.C.S. Oliveira, Embrapa Pecuária Sudeste.
dc.contributor.author.fl_str_mv REGITANO, L. C. A.
IBELLI, A. M. G.
GASPARIN, G.
MIYATA, M.
AZEVEDO, A. L. S.
COUTINHO, L. L.
TEODORO, R. L.
MACHADO, M. A.
SILVA, M. V. G. B.
NAKATA, L. C.
ZAROS, L. G.
SONSTEGARD, T. S.
SILVA, A. M.
ALENCAR, M. M.
OLIVEIRA, M. C. S.
dc.subject.por.fl_str_mv Marcadores genéticos
Carrapato
Resistência
topic Marcadores genéticos
Carrapato
Resistência
description Genetic differences in susceptibility to tick (Riphicephalus (Boophilus) microplus) are considerable in bovine. In general, Bos taurus is more susceptible to ticks than Bos indicus. On the search for the molecular basis of tick resistance we used mapping, association and gene expression approaches. A B. taurus x B. indicus F2 population was developed by Embrapa and 300 individuals were measured for parasite load and genotype for microsatellite markers covering chromosomes 5, 7 and 14. A suggestive chromosome-wide QTL for tick load (P < 0.05) was mapped on chromosome 5 and two suggestive (P < 0.10) QTL on chromosome 7 and 14. In the candidate gene approach, females from the genetic groups Nelore (NE -184), Canchim x Nelore (CN -153), Aberdeen Angus x Nelore (AN -123) and Simmental x Nelore (SN -120) were evaluated under natural infestation in seven to ten tick Counts. Microsatellite markers close to the genes for interleukin 2 (IL2), interleukin 4 (IL4) and interferongamma (lFNG) were analysed. Tick counts were associated to the marker for interleukin 4 (P < 0.05) in NE, CN and AN genetic groups. We also investigated if there were differences in cytokine mRNA of naive versus infested Nelore as well as of resistant versus susceptible cows from NE, CN and AN genetic groups. Relative quantification of cytokines from infested compared to naives showed down regulation of IL2. When resistant cows from the from genetic groups were. compared to susceptible animals, IL8 was down regulated but no differences were found for IL4 expression.
publishDate 2008
dc.date.none.fl_str_mv 2008
2009-02-19
2024-06-17T12:55:14Z
2024-06-17T12:55:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.uri.fl_str_mv In: PINARD, M.; GAY, C.; PASTORET, P.; DODET, B. (Ed.). Animal genomics for animal healt. Basel, Karger: Dev Biol, 2008. p. 225-230.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/596319
identifier_str_mv In: PINARD, M.; GAY, C.; PASTORET, P.; DODET, B. (Ed.). Animal genomics for animal healt. Basel, Karger: Dev Biol, 2008. p. 225-230.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/596319
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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