Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR.
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154376 http://doi.org/10.1590/0103-8478cr20210328 |
Resumo: | The aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis. |
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Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR.ERIC 1+2-PCREpidemiologia molecularGenotipagemLinfadenite CaseosaDoença AnimalMolecular epidemiologyCaseous lymphadenitisGenotypingThe aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis.CAROLINA PANTUZZA RAMOS, UFMG; ELAINE MARIA SELES DORNELES, UFLA; DIONEI JOAQUIM HAAS, UFMG; JOSIR LAINE APARECIDA VESCHI, CPATSA; DAN LOUREIRO, UFBA; RICARDO DIAS PORTELA, UFBA; VASCO AZEVEDO, UFMG; MARCOS BRYAN HEINEMANN, USP - São Paulo; ANDREY PEREIRA LAGE, UFMG.RAMOS, C. P.DORNELES, E. M. S.HAAS, D. J.VESCHI, J. L. A.LOUREIRO, D.PORTELA, R. D.AZEVEDO, V.HEINEMANN, M. B.LAGE, A. P.2023-06-12T12:33:18Z2023-06-12T12:33:18Z2023-06-122022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCiência Rural, v. 52, n. 11, e20210328, 2022.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154376http://doi.org/10.1590/0103-8478cr20210328enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-06-12T12:33:18Zoai:www.alice.cnptia.embrapa.br:doc/1154376Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-06-12T12:33:18falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-06-12T12:33:18Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
title |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
spellingShingle |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. RAMOS, C. P. ERIC 1+2-PCR Epidemiologia molecular Genotipagem Linfadenite Caseosa Doença Animal Molecular epidemiology Caseous lymphadenitis Genotyping |
title_short |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
title_full |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
title_fullStr |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
title_full_unstemmed |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
title_sort |
Molecular characterization of Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis by ERIC-PCR. |
author |
RAMOS, C. P. |
author_facet |
RAMOS, C. P. DORNELES, E. M. S. HAAS, D. J. VESCHI, J. L. A. LOUREIRO, D. PORTELA, R. D. AZEVEDO, V. HEINEMANN, M. B. LAGE, A. P. |
author_role |
author |
author2 |
DORNELES, E. M. S. HAAS, D. J. VESCHI, J. L. A. LOUREIRO, D. PORTELA, R. D. AZEVEDO, V. HEINEMANN, M. B. LAGE, A. P. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
CAROLINA PANTUZZA RAMOS, UFMG; ELAINE MARIA SELES DORNELES, UFLA; DIONEI JOAQUIM HAAS, UFMG; JOSIR LAINE APARECIDA VESCHI, CPATSA; DAN LOUREIRO, UFBA; RICARDO DIAS PORTELA, UFBA; VASCO AZEVEDO, UFMG; MARCOS BRYAN HEINEMANN, USP - São Paulo; ANDREY PEREIRA LAGE, UFMG. |
dc.contributor.author.fl_str_mv |
RAMOS, C. P. DORNELES, E. M. S. HAAS, D. J. VESCHI, J. L. A. LOUREIRO, D. PORTELA, R. D. AZEVEDO, V. HEINEMANN, M. B. LAGE, A. P. |
dc.subject.por.fl_str_mv |
ERIC 1+2-PCR Epidemiologia molecular Genotipagem Linfadenite Caseosa Doença Animal Molecular epidemiology Caseous lymphadenitis Genotyping |
topic |
ERIC 1+2-PCR Epidemiologia molecular Genotipagem Linfadenite Caseosa Doença Animal Molecular epidemiology Caseous lymphadenitis Genotyping |
description |
The aims of the present study were (i) to genotype Corynebacterium pseudotuberculosis, C. silvaticum, and C. auriscanis strains using enterobacterial repetitive intergenic consensus (ERIC-PCR), and (ii) to analyze the epidemiological relationships among isolates according to biovar (Equi and Ovis), species, host, and geographical origin of the C. pseudotuberculosis strains. Sixty-eight C. pseudotuberculosis, nine C. silvaticum, and one C. auriscanis, C. pseudotuberculosis ATCC® 19410? strain and the attenuated C. pseudotuberculosis 1002 vaccinal strain were fingerprinted by ERIC 1+2-PCR. Field strains were isolated from various hosts (cattle, buffaloes, sheep, goats, horses, dogs, and pigs) in six countries (Mexico, Portugal, Brazil, Equatorial Guinea, Egypt, and Israel). High genetic diversity was found among the studied Corynebacterium spp. isolates, clustering in 24 genotypes with a Hunter & Gaston diversity index (HGDI) of 0.937. The minimal spanning tree of Corynebacterium spp. revealed three clonal complexes, each associated with one bacterial species. Twenty-two genotypes were observed among C. pseudotuberculosis isolates, with an HGDI of 0.934. Three major clonal complexes were formed at the minimal spanning tree, grouped around the geographic origin of C. pseudotuberculosis isolates. These results reinforce the high typeability, epidemiological concordance, and discriminatory power of ERIC-PCR as a consistent genotyping method for C. pseudotuberculosis, which could be useful as an epidemiological tool to control caseous lymphadenitis. Moreover, our results also indicate the potential of ERIC 1+2-PCR for the genotyping of other species of Corynebacterium other than C. pseudotuberculosis. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2023-06-12T12:33:18Z 2023-06-12T12:33:18Z 2023-06-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Ciência Rural, v. 52, n. 11, e20210328, 2022. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154376 http://doi.org/10.1590/0103-8478cr20210328 |
identifier_str_mv |
Ciência Rural, v. 52, n. 11, e20210328, 2022. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1154376 http://doi.org/10.1590/0103-8478cr20210328 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503545806913536 |