Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil.
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152548 |
Resumo: | Citrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (Citrus sinensis L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(?)RNA viruses, with ORFs in a typical organization of members of the genus Dichorhavirus, were recovered. These genomes shared 98?99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for new species demarcation within that genus. Phylogenetically, the three haplotypes of the new virus called citrus bright spot virus (CiBSV) are clustered with citrus leprosis virus N, which is a dichorhavirus transmitted by Brevipalpus phoenicis sensu stricto. In CiBSV-infected citrus plants, B. papayensis and B. azores were found, but the virus could only be transmitted to Arabidopsis plants by B. azores. The study provides the first evidence of the role of B. azores as a viral vector and supports the assignment of CiBSV to the tentative new species Dichorhavirus australis. |
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spelling |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil.Fruta CítricaVírusMononegaviralesRhabdoviridaeVector-borne diseasesCitrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (Citrus sinensis L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(?)RNA viruses, with ORFs in a typical organization of members of the genus Dichorhavirus, were recovered. These genomes shared 98?99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for new species demarcation within that genus. Phylogenetically, the three haplotypes of the new virus called citrus bright spot virus (CiBSV) are clustered with citrus leprosis virus N, which is a dichorhavirus transmitted by Brevipalpus phoenicis sensu stricto. In CiBSV-infected citrus plants, B. papayensis and B. azores were found, but the virus could only be transmitted to Arabidopsis plants by B. azores. The study provides the first evidence of the role of B. azores as a viral vector and supports the assignment of CiBSV to the tentative new species Dichorhavirus australis.CAMILA CHABI-JESUS, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; PEDRO LUIS RAMOS-GONZÁLEZ, INSTITUTO BIOLÓGICO; ALINE DANIELE TASSI, INSTITUTO BIOLÓGICO; LAURA ROSSETTO PEREIRA, INSTITUTO BIOLÓGICO; MARINÊS BASTIANEL, CENTRO DE CITRICULTURA SYLVIO MOREIRA; DOUGLAS LAU, CNPT; MARIA CRISTINA CANALE, EMPRESA DE PESQUISA AGROPECUÁRIA E EXTENSÃO RURAL DE SANTA CATARINA; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; VALDENICE MOREIRA NOVELLI, CENTRO DE CITRICULTURA SYLVIO MOREIRA; ELLIOT WATA NABE KITAJIMA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JULIANA DE FREITAS ASTUA, CNPMF.CHABI-JESUS, C.RAMOS-GONZÁLEZ, P. L.TASSI, A. D.PEREIRA, L. R.BASTIANEL, M.LAU, D.CANALE, M. C.HARAKAVA, R.NOVELLI, V. M.KITAJIMA, E. W. N.ASTUA, J. de F.2023-03-21T14:50:58Z2023-03-21T14:50:58Z2023-03-212023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlants, v.12, n.6 p.1371. 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/115254810.3390/plants12061371enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-03-21T14:50:58Zoai:www.alice.cnptia.embrapa.br:doc/1152548Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-03-21T14:50:58falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-03-21T14:50:58Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. |
title |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. |
spellingShingle |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. CHABI-JESUS, C. Fruta Cítrica Vírus Mononegavirales Rhabdoviridae Vector-borne diseases |
title_short |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. |
title_full |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. |
title_fullStr |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. |
title_full_unstemmed |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. |
title_sort |
Citrus bright spot virus: a new dichorhavirus, transmitted by Brevipalpus azores, Causing Citrus Leprosis Disease in Brazil. |
author |
CHABI-JESUS, C. |
author_facet |
CHABI-JESUS, C. RAMOS-GONZÁLEZ, P. L. TASSI, A. D. PEREIRA, L. R. BASTIANEL, M. LAU, D. CANALE, M. C. HARAKAVA, R. NOVELLI, V. M. KITAJIMA, E. W. N. ASTUA, J. de F. |
author_role |
author |
author2 |
RAMOS-GONZÁLEZ, P. L. TASSI, A. D. PEREIRA, L. R. BASTIANEL, M. LAU, D. CANALE, M. C. HARAKAVA, R. NOVELLI, V. M. KITAJIMA, E. W. N. ASTUA, J. de F. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
CAMILA CHABI-JESUS, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; PEDRO LUIS RAMOS-GONZÁLEZ, INSTITUTO BIOLÓGICO; ALINE DANIELE TASSI, INSTITUTO BIOLÓGICO; LAURA ROSSETTO PEREIRA, INSTITUTO BIOLÓGICO; MARINÊS BASTIANEL, CENTRO DE CITRICULTURA SYLVIO MOREIRA; DOUGLAS LAU, CNPT; MARIA CRISTINA CANALE, EMPRESA DE PESQUISA AGROPECUÁRIA E EXTENSÃO RURAL DE SANTA CATARINA; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; VALDENICE MOREIRA NOVELLI, CENTRO DE CITRICULTURA SYLVIO MOREIRA; ELLIOT WATA NABE KITAJIMA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JULIANA DE FREITAS ASTUA, CNPMF. |
dc.contributor.author.fl_str_mv |
CHABI-JESUS, C. RAMOS-GONZÁLEZ, P. L. TASSI, A. D. PEREIRA, L. R. BASTIANEL, M. LAU, D. CANALE, M. C. HARAKAVA, R. NOVELLI, V. M. KITAJIMA, E. W. N. ASTUA, J. de F. |
dc.subject.por.fl_str_mv |
Fruta Cítrica Vírus Mononegavirales Rhabdoviridae Vector-borne diseases |
topic |
Fruta Cítrica Vírus Mononegavirales Rhabdoviridae Vector-borne diseases |
description |
Citrus leprosis (CL) is the main viral disease affecting the Brazilian citriculture. Sweet orange (Citrus sinensis L. Osbeck) trees affected by CL were identified in small orchards in Southern Brazil. Rod-like particles of 40 × 100 nm and electron lucent viroplasm were observed in the nucleus of infected cells in symptomatic tissues. RNA extracts from three plants, which proved negative by RT-PCR for known CL-causing viruses, were analyzed by high throughput sequencing and Sanger sequencing after RT-PCR. The genomes of bi-segmented ss(?)RNA viruses, with ORFs in a typical organization of members of the genus Dichorhavirus, were recovered. These genomes shared 98?99% nt sequence identity among them but <73% with those of known dichorhavirids, a value below the threshold for new species demarcation within that genus. Phylogenetically, the three haplotypes of the new virus called citrus bright spot virus (CiBSV) are clustered with citrus leprosis virus N, which is a dichorhavirus transmitted by Brevipalpus phoenicis sensu stricto. In CiBSV-infected citrus plants, B. papayensis and B. azores were found, but the virus could only be transmitted to Arabidopsis plants by B. azores. The study provides the first evidence of the role of B. azores as a viral vector and supports the assignment of CiBSV to the tentative new species Dichorhavirus australis. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-21T14:50:58Z 2023-03-21T14:50:58Z 2023-03-21 2023 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plants, v.12, n.6 p.1371. 2023. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152548 10.3390/plants12061371 |
identifier_str_mv |
Plants, v.12, n.6 p.1371. 2023. 10.3390/plants12061371 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1152548 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503541688107008 |