Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).

Detalhes bibliográficos
Autor(a) principal: ROCHA, M. de F.
Data de Publicação: 2011
Outros Autores: MELO, N. F. de, SOUZA, M. J. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/889967
Resumo: The grasshopper species Orthoscapheus rufipes and Eujivarus fusiformis were analyzed using several cytogenetic techniques. The karyotype of O. rufipes, described here for the first time, had a diploid number of 2n = 23, whereasE. fusiformis had a karyotype with 2n = 21. The two species showed the same mechanism of sex determination (XO type) but differed in chromosome morphology. Pericentromeric blocks of constitutive heterochromatin (CH) were de- tected in the chromosome complement of both species. CMA3 /DA/DAPI staining revealed CMA3 -positive blocks in CH regions in four autosomal bivalents of O. rufipes and in two of E. fusiformis. The location of active NORs differed between the two species, occurring in bivalents M6 and S9 of O. rufipes and M6 and M7 of E. fusiformsi. The rDNA sites revealed by FISH coincided with the number and position of the active NORs detected by AgNO 3 staining. The vari- ability in chromosomal markers accounted for the karyotype differentiation observed in the tribe Abracrini.
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spelling Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).Análise citogenéticaHeterocromatina constitutivaFluorocromosDNAGafanhotoOrthopteraThe grasshopper species Orthoscapheus rufipes and Eujivarus fusiformis were analyzed using several cytogenetic techniques. The karyotype of O. rufipes, described here for the first time, had a diploid number of 2n = 23, whereasE. fusiformis had a karyotype with 2n = 21. The two species showed the same mechanism of sex determination (XO type) but differed in chromosome morphology. Pericentromeric blocks of constitutive heterochromatin (CH) were de- tected in the chromosome complement of both species. CMA3 /DA/DAPI staining revealed CMA3 -positive blocks in CH regions in four autosomal bivalents of O. rufipes and in two of E. fusiformis. The location of active NORs differed between the two species, occurring in bivalents M6 and S9 of O. rufipes and M6 and M7 of E. fusiformsi. The rDNA sites revealed by FISH coincided with the number and position of the active NORs detected by AgNO 3 staining. The vari- ability in chromosomal markers accounted for the karyotype differentiation observed in the tribe Abracrini.MARÍLIA DE FRANÇA ROCHA, UPE; NATONIEL FRANKLIN DE MELO, CPATSA; MARIA JOSÉ DE SOUZA, UFPE.ROCHA, M. de F.MELO, N. F. deSOUZA, M. J. de2011-05-30T11:11:11Z2011-05-30T11:11:11Z2011-05-3020112011-08-08T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Biology, Ribeirão Preto, v. 34, n. 2, p. 214-219, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/889967enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T23:38:50Zoai:www.alice.cnptia.embrapa.br:doc/889967Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T23:38:50falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T23:38:50Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).
title Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).
spellingShingle Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).
ROCHA, M. de F.
Análise citogenética
Heterocromatina constitutiva
Fluorocromos
DNA
Gafanhoto
Orthoptera
title_short Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).
title_full Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).
title_fullStr Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).
title_full_unstemmed Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).
title_sort Comparative cytogenetic analysis of two grasshopper species of the tribe Abracrini (Ommatolampinae, Acrididae).
author ROCHA, M. de F.
author_facet ROCHA, M. de F.
MELO, N. F. de
SOUZA, M. J. de
author_role author
author2 MELO, N. F. de
SOUZA, M. J. de
author2_role author
author
dc.contributor.none.fl_str_mv MARÍLIA DE FRANÇA ROCHA, UPE; NATONIEL FRANKLIN DE MELO, CPATSA; MARIA JOSÉ DE SOUZA, UFPE.
dc.contributor.author.fl_str_mv ROCHA, M. de F.
MELO, N. F. de
SOUZA, M. J. de
dc.subject.por.fl_str_mv Análise citogenética
Heterocromatina constitutiva
Fluorocromos
DNA
Gafanhoto
Orthoptera
topic Análise citogenética
Heterocromatina constitutiva
Fluorocromos
DNA
Gafanhoto
Orthoptera
description The grasshopper species Orthoscapheus rufipes and Eujivarus fusiformis were analyzed using several cytogenetic techniques. The karyotype of O. rufipes, described here for the first time, had a diploid number of 2n = 23, whereasE. fusiformis had a karyotype with 2n = 21. The two species showed the same mechanism of sex determination (XO type) but differed in chromosome morphology. Pericentromeric blocks of constitutive heterochromatin (CH) were de- tected in the chromosome complement of both species. CMA3 /DA/DAPI staining revealed CMA3 -positive blocks in CH regions in four autosomal bivalents of O. rufipes and in two of E. fusiformis. The location of active NORs differed between the two species, occurring in bivalents M6 and S9 of O. rufipes and M6 and M7 of E. fusiformsi. The rDNA sites revealed by FISH coincided with the number and position of the active NORs detected by AgNO 3 staining. The vari- ability in chromosomal markers accounted for the karyotype differentiation observed in the tribe Abracrini.
publishDate 2011
dc.date.none.fl_str_mv 2011-05-30T11:11:11Z
2011-05-30T11:11:11Z
2011-05-30
2011
2011-08-08T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genetics and Molecular Biology, Ribeirão Preto, v. 34, n. 2, p. 214-219, 2011.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/889967
identifier_str_mv Genetics and Molecular Biology, Ribeirão Preto, v. 34, n. 2, p. 214-219, 2011.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/889967
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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