A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.

Detalhes bibliográficos
Autor(a) principal: VASCONCELOS, E. A. R.
Data de Publicação: 2011
Outros Autores: SANTANA, C. G., GODOY, C. V., SEIXAS, C. D. S., SILVA, M. S., MOREIRA, L. R. S., OLIVEIRA-NETO, O. B., PRICE, D., FITCHES, E., FERREIRA FILHO, E. X., MEHTA, A., GATEHOUSE, J. A., GROSSI-DE-SA, M. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/879641
Resumo: Background: Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. Results: A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (b/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay showed that purified recombinant CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at 12:1 (w/w) enzyme:inhibitor ratio. Additionally, CaclXIP at 1.5 ?g/?L inhibited the germination of spores of Phakopsora pachyrhizi by 45%. Conclusions: Our data suggests that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes it an eligible candidate gene for the control of Asian rust.
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spelling A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.SojaDoença de plantaSoybeansPlant diseases and disordersBackground: Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. Results: A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (b/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay showed that purified recombinant CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at 12:1 (w/w) enzyme:inhibitor ratio. Additionally, CaclXIP at 1.5 ?g/?L inhibited the germination of spores of Phakopsora pachyrhizi by 45%. Conclusions: Our data suggests that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes it an eligible candidate gene for the control of Asian rust.ERICO A. R. VASCONCELOS, Embrapa Recursos Genéticos e Biotecnologia; CELSO G. SANTANA, Embrapa Recursos Genéticos e Biotecnologia; CLAUDIA VIEIRA GODOY, CNPSO; CLAUDINE DINALI SANTOS SEIXAS, CNPSO; MARILIA SANTOS SILVA, CPAC; LEONORA R. S. MOREIRA, UNB; OSMUNDO B. OLIVEIRA-NETO, Embrapa Recursos Genéticos e Biotecnologia; DANIEL PRICE, Durham University; ELAINE FITCHES, Durham University; EDIVALDO XIMENES FERREIRA FILHO, UNB; ANGELA MEHTA DOS REIS, CENARGEN; JOHN A. GATEHOUSE, Durham University; MARIA FATIMA GROSSI DE SA, CENARGEN.VASCONCELOS, E. A. R.SANTANA, C. G.GODOY, C. V.SEIXAS, C. D. S.SILVA, M. S.MOREIRA, L. R. S.OLIVEIRA-NETO, O. B.PRICE, D.FITCHES, E.FERREIRA FILHO, E. X.MEHTA, A.GATEHOUSE, J. A.GROSSI-DE-SA, M. F.2011-04-10T11:11:11Z2011-04-10T11:11:11Z2011-03-0120112018-04-17T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBMC Biotechnology, v. 11, 2011.http://www.alice.cnptia.embrapa.br/alice/handle/doc/879641enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:12:51Zoai:www.alice.cnptia.embrapa.br:doc/879641Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:12:51falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:12:51Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
title A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
spellingShingle A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
VASCONCELOS, E. A. R.
Soja
Doença de planta
Soybeans
Plant diseases and disorders
title_short A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
title_full A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
title_fullStr A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
title_full_unstemmed A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
title_sort A new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) affects Soybean Asian rust (Phakopsora pachyrhizi) spore germination.
author VASCONCELOS, E. A. R.
author_facet VASCONCELOS, E. A. R.
SANTANA, C. G.
GODOY, C. V.
SEIXAS, C. D. S.
SILVA, M. S.
MOREIRA, L. R. S.
OLIVEIRA-NETO, O. B.
PRICE, D.
FITCHES, E.
FERREIRA FILHO, E. X.
MEHTA, A.
GATEHOUSE, J. A.
GROSSI-DE-SA, M. F.
author_role author
author2 SANTANA, C. G.
GODOY, C. V.
SEIXAS, C. D. S.
SILVA, M. S.
MOREIRA, L. R. S.
OLIVEIRA-NETO, O. B.
PRICE, D.
FITCHES, E.
FERREIRA FILHO, E. X.
MEHTA, A.
GATEHOUSE, J. A.
GROSSI-DE-SA, M. F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv ERICO A. R. VASCONCELOS, Embrapa Recursos Genéticos e Biotecnologia; CELSO G. SANTANA, Embrapa Recursos Genéticos e Biotecnologia; CLAUDIA VIEIRA GODOY, CNPSO; CLAUDINE DINALI SANTOS SEIXAS, CNPSO; MARILIA SANTOS SILVA, CPAC; LEONORA R. S. MOREIRA, UNB; OSMUNDO B. OLIVEIRA-NETO, Embrapa Recursos Genéticos e Biotecnologia; DANIEL PRICE, Durham University; ELAINE FITCHES, Durham University; EDIVALDO XIMENES FERREIRA FILHO, UNB; ANGELA MEHTA DOS REIS, CENARGEN; JOHN A. GATEHOUSE, Durham University; MARIA FATIMA GROSSI DE SA, CENARGEN.
dc.contributor.author.fl_str_mv VASCONCELOS, E. A. R.
SANTANA, C. G.
GODOY, C. V.
SEIXAS, C. D. S.
SILVA, M. S.
MOREIRA, L. R. S.
OLIVEIRA-NETO, O. B.
PRICE, D.
FITCHES, E.
FERREIRA FILHO, E. X.
MEHTA, A.
GATEHOUSE, J. A.
GROSSI-DE-SA, M. F.
dc.subject.por.fl_str_mv Soja
Doença de planta
Soybeans
Plant diseases and disorders
topic Soja
Doença de planta
Soybeans
Plant diseases and disorders
description Background: Asian rust (Phakopsora pachyrhizi) is a common disease in Brazilian soybean fields and it is difficult to control. To identify a biochemical candidate with potential to combat this disease, a new chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP) leaves was cloned into the pGAPZa-B vector for expression in Pichia pastoris. Results: A cDNA encoding a chitinase-like xylanase inhibitor protein (XIP) from coffee (Coffea arabica) (CaclXIP), was isolated from leaves. The amino acid sequence predicts a (b/a)8 topology common to Class III Chitinases (glycoside hydrolase family 18 proteins; GH18), and shares similarity with other GH18 members, although it lacks the glutamic acid residue essential for catalysis, which is replaced by glutamine. CaclXIP was expressed as a recombinant protein in Pichia pastoris. Enzymatic assay showed that purified recombinant CaclXIP had only residual chitinolytic activity. However, it inhibited xylanases from Acrophialophora nainiana by approx. 60% when present at 12:1 (w/w) enzyme:inhibitor ratio. Additionally, CaclXIP at 1.5 ?g/?L inhibited the germination of spores of Phakopsora pachyrhizi by 45%. Conclusions: Our data suggests that CaclXIP belongs to a class of naturally inactive chitinases that have evolved to act in plant cell defence as xylanase inhibitors. Its role on inhibiting germination of fungal spores makes it an eligible candidate gene for the control of Asian rust.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-10T11:11:11Z
2011-04-10T11:11:11Z
2011-03-01
2011
2018-04-17T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BMC Biotechnology, v. 11, 2011.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/879641
identifier_str_mv BMC Biotechnology, v. 11, 2011.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/879641
dc.language.iso.fl_str_mv eng
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