Development of mutants of Coniothyrium minitans with improved efficiency for control of Sclerotinia sclerotiorum.

• Autor(a) principal: MELO, I. S. de
• Data de Publicação: 2011
• Outros Autores: MORETINI, A., CASSIOLATO, A. M. R., FAULL, J. L.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/898276
• topic: Coniothvrium minitans; Resistance; Antibiotic production; Sclerotinia sclerotiorum; Doença fúngica; Controle biológico; Fungo para controle biológico; Biological control; Antifungal agents; iprodione

Coniothurium minitans (CM) is hyperparasitic to Sclerotinia sderotiorum (55), a palhogen of many economically important crops. In this paper, we describe lhe isolation of improved mutants of CM, using a UV - irradiation regime, with altered chitinase production and tolerance to high concentration of iprodione, which are effect:iveagainst Ss. Three out of the 59 mutants obtained inhibited the mycelial growth of CM. Infectivity of sclerotia by the new mutants was assayed by the plant-tissue-based system using carrot segments. More than 80%of sclerotia were colonized by lhe mutants and the wild-type CM. The mutant strains retained ability to produce significant amounts of chitinase. The mutants differed frorn their wild-type strain in appearance, morphology and sporulation. In conclusion, the results presented here provide evidence that lhe new biotypes of C. minitans are effective in controlling S.sclemtiarum.