Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.

Detalhes bibliográficos
Autor(a) principal: CARVALHO, L. J. C. B.
Data de Publicação: 2016
Outros Autores: AGUSTINI, M. A. V., ANDERSON, J. V., VIEIRA, E. A., SOUZA, C. R. B. de, CHEN, S., SCHAAL, B. A., SILVA, J. P. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1051346
Resumo: ABSTRACT: BACKGROUND: Cassava (Manihot esculenta Crantz) storage root provides a staple food source for millions of people worldwide. Increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. Because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-yellow-to-pink. This study provides important information to plant breeding programs aimed at improving cassava storage root nutritional quality. RESULTS: Among the 23 landraces, five carotenoid types were detected in storage root with white color, while carotenoid types ranged from 1 to 21 in storage root with pink and yellow color. The majority of storage root in these landraces ranged in color from pale-to-intense yellow. In this color group, total ß-carotene, containing all-E-, 9-Z-, and 13-Z-ß-carotene isomers, was the major carotenoid type detected, varying from 26.13 to 76.72 %. Although no ?-carotene was observed, variable amounts of a ?-ring derived xanthophyll, lutein, was detected; with greater accumulation of ?-ring xanthophylls than of ß-ring xanthophyll. Lycopene was detected in a landrace (Cas51) with pink color storage root, but it was not detected in storage root with yellow color. Based on microarray and qRT-PCR analyses, abundance of transcripts coding for enzymes involved in carotenoid biosynthesis were consistent with carotenoid composition determined by contrasting HPLC-Diode Array profiles from storage root of landraces IAC12, Cas64, and Cas51. Abundance of transcripts encoding for proteins regulating plastid division were also consistent with the observed differences in total ß-carotene accumulation. CONCLUSIONS: Among the 23 cassava landraces with varying storage root color and diverse carotenoid types and profiles, landrace Cas51 (pink color storage root) had low LYCb transcript abundance, whereas landrace Cas64 (intense yellow storage root) had decreased HYb transcript abundance. These results may explain the increased amounts of lycopene and total ß-carotene observed in landraces Cas51 and Cas64, respectively. Overall, total carotenoid content in cassava storage root of color class representatives were associated with spatial patterns of secondary growth, color, and abundance of transcripts linked to plastid division. Finally, a partial carotenoid biosynthesis pathway is proposed.
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spelling Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.CaretonóideHPLC-DADMicromatrizes de DNAQRT_PCRMandiocaArmazenamentoCassavaStorageCarotenoidsDNA microarraysABSTRACT: BACKGROUND: Cassava (Manihot esculenta Crantz) storage root provides a staple food source for millions of people worldwide. Increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. Because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-yellow-to-pink. This study provides important information to plant breeding programs aimed at improving cassava storage root nutritional quality. RESULTS: Among the 23 landraces, five carotenoid types were detected in storage root with white color, while carotenoid types ranged from 1 to 21 in storage root with pink and yellow color. The majority of storage root in these landraces ranged in color from pale-to-intense yellow. In this color group, total ß-carotene, containing all-E-, 9-Z-, and 13-Z-ß-carotene isomers, was the major carotenoid type detected, varying from 26.13 to 76.72 %. Although no ?-carotene was observed, variable amounts of a ?-ring derived xanthophyll, lutein, was detected; with greater accumulation of ?-ring xanthophylls than of ß-ring xanthophyll. Lycopene was detected in a landrace (Cas51) with pink color storage root, but it was not detected in storage root with yellow color. Based on microarray and qRT-PCR analyses, abundance of transcripts coding for enzymes involved in carotenoid biosynthesis were consistent with carotenoid composition determined by contrasting HPLC-Diode Array profiles from storage root of landraces IAC12, Cas64, and Cas51. Abundance of transcripts encoding for proteins regulating plastid division were also consistent with the observed differences in total ß-carotene accumulation. CONCLUSIONS: Among the 23 cassava landraces with varying storage root color and diverse carotenoid types and profiles, landrace Cas51 (pink color storage root) had low LYCb transcript abundance, whereas landrace Cas64 (intense yellow storage root) had decreased HYb transcript abundance. These results may explain the increased amounts of lycopene and total ß-carotene observed in landraces Cas51 and Cas64, respectively. Overall, total carotenoid content in cassava storage root of color class representatives were associated with spatial patterns of secondary growth, color, and abundance of transcripts linked to plastid division. Finally, a partial carotenoid biosynthesis pathway is proposed.LUIZ JOAQUIM CASTELO B CARVALHO, Cenargen; MARCO A. V. AGUSTINI, Cenargen; JAMES V. ANDERSON, USDA ARS; EDUARDO ALANO VIEIRA, CPAC; CLAUDIA R. B. DE SOUZA, UFPA; SONGBI CHEN, Chinese Acad Trop Agr, Trop Crops Genet Resources Inst; BARBARA A. SCHAAL, Washington Univ; JOSEANE PADILHA DA SILVA, Cenargen.CARVALHO, L. J. C. B.AGUSTINI, M. A. V.ANDERSON, J. V.VIEIRA, E. A.SOUZA, C. R. B. deCHEN, S.SCHAAL, B. A.SILVA, J. P. da2016-08-23T08:11:43Z2016-08-23T08:11:43Z2016-08-2220162016-08-23T08:11:43Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBMC Plant Biology, v. 16, n. 1, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/105134610.1186/s12870-016-0826-0enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T02:41:58Zoai:www.alice.cnptia.embrapa.br:doc/1051346Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T02:41:58falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T02:41:58Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.
title Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.
spellingShingle Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.
CARVALHO, L. J. C. B.
Caretonóide
HPLC-DAD
Micromatrizes de DNA
QRT_PCR
Mandioca
Armazenamento
Cassava
Storage
Carotenoids
DNA microarrays
title_short Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.
title_full Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.
title_fullStr Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.
title_full_unstemmed Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.
title_sort Natural variation in expression of genes associated with carotenoid biosynthesis and accumulation in cassava (Manihot esculenta Crantz) storage root.
author CARVALHO, L. J. C. B.
author_facet CARVALHO, L. J. C. B.
AGUSTINI, M. A. V.
ANDERSON, J. V.
VIEIRA, E. A.
SOUZA, C. R. B. de
CHEN, S.
SCHAAL, B. A.
SILVA, J. P. da
author_role author
author2 AGUSTINI, M. A. V.
ANDERSON, J. V.
VIEIRA, E. A.
SOUZA, C. R. B. de
CHEN, S.
SCHAAL, B. A.
SILVA, J. P. da
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv LUIZ JOAQUIM CASTELO B CARVALHO, Cenargen; MARCO A. V. AGUSTINI, Cenargen; JAMES V. ANDERSON, USDA ARS; EDUARDO ALANO VIEIRA, CPAC; CLAUDIA R. B. DE SOUZA, UFPA; SONGBI CHEN, Chinese Acad Trop Agr, Trop Crops Genet Resources Inst; BARBARA A. SCHAAL, Washington Univ; JOSEANE PADILHA DA SILVA, Cenargen.
dc.contributor.author.fl_str_mv CARVALHO, L. J. C. B.
AGUSTINI, M. A. V.
ANDERSON, J. V.
VIEIRA, E. A.
SOUZA, C. R. B. de
CHEN, S.
SCHAAL, B. A.
SILVA, J. P. da
dc.subject.por.fl_str_mv Caretonóide
HPLC-DAD
Micromatrizes de DNA
QRT_PCR
Mandioca
Armazenamento
Cassava
Storage
Carotenoids
DNA microarrays
topic Caretonóide
HPLC-DAD
Micromatrizes de DNA
QRT_PCR
Mandioca
Armazenamento
Cassava
Storage
Carotenoids
DNA microarrays
description ABSTRACT: BACKGROUND: Cassava (Manihot esculenta Crantz) storage root provides a staple food source for millions of people worldwide. Increasing the carotenoid content in storage root of cassava could provide improved nutritional and health benefits. Because carotenoid accumulation has been associated with storage root color, this study characterized carotenoid profiles, and abundance of key transcripts associated with carotenoid biosynthesis, from 23 landraces of cassava storage root ranging in color from white-to-yellow-to-pink. This study provides important information to plant breeding programs aimed at improving cassava storage root nutritional quality. RESULTS: Among the 23 landraces, five carotenoid types were detected in storage root with white color, while carotenoid types ranged from 1 to 21 in storage root with pink and yellow color. The majority of storage root in these landraces ranged in color from pale-to-intense yellow. In this color group, total ß-carotene, containing all-E-, 9-Z-, and 13-Z-ß-carotene isomers, was the major carotenoid type detected, varying from 26.13 to 76.72 %. Although no ?-carotene was observed, variable amounts of a ?-ring derived xanthophyll, lutein, was detected; with greater accumulation of ?-ring xanthophylls than of ß-ring xanthophyll. Lycopene was detected in a landrace (Cas51) with pink color storage root, but it was not detected in storage root with yellow color. Based on microarray and qRT-PCR analyses, abundance of transcripts coding for enzymes involved in carotenoid biosynthesis were consistent with carotenoid composition determined by contrasting HPLC-Diode Array profiles from storage root of landraces IAC12, Cas64, and Cas51. Abundance of transcripts encoding for proteins regulating plastid division were also consistent with the observed differences in total ß-carotene accumulation. CONCLUSIONS: Among the 23 cassava landraces with varying storage root color and diverse carotenoid types and profiles, landrace Cas51 (pink color storage root) had low LYCb transcript abundance, whereas landrace Cas64 (intense yellow storage root) had decreased HYb transcript abundance. These results may explain the increased amounts of lycopene and total ß-carotene observed in landraces Cas51 and Cas64, respectively. Overall, total carotenoid content in cassava storage root of color class representatives were associated with spatial patterns of secondary growth, color, and abundance of transcripts linked to plastid division. Finally, a partial carotenoid biosynthesis pathway is proposed.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-23T08:11:43Z
2016-08-23T08:11:43Z
2016-08-22
2016
2016-08-23T08:11:43Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BMC Plant Biology, v. 16, n. 1, 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1051346
10.1186/s12870-016-0826-0
identifier_str_mv BMC Plant Biology, v. 16, n. 1, 2016.
10.1186/s12870-016-0826-0
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1051346
dc.language.iso.fl_str_mv eng
language eng
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instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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repository.mail.fl_str_mv cg-riaa@embrapa.br
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