Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata.
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002535 http://dx.doi.org/10.4238/2014.January.28.6 |
Resumo: | 17-α-methyltestosterone (MT) is a synthetic hormone used in fish hatcheries to induce male monosex. Snails hold promise as possible test models to assess chemicals acting on the endocrine system. Biomphalaria glabrata is an aquatic gastropod mollusk (Pulmonata, Planorbidae) that can be easily maintained in aquaria, predisposing the species for use in ecotoxicological testing. This study evaluated the reproductive effects of MT on B. glabrata by examining histological changes and its reproductive performance. Ten snails per group were exposed for 4 weeks to different concentrations of MT (0.01, 0.1, and 1.0 mg/L). The total number of laid eggs, egg mass per group, size of type V oocytes, and production of spermatozoids were determined. Reproduction of B. glabrata was affected by MT. At the lowest concentration (0.01 mg/L), MT caused a statistically significant increase in the number of egg mass per snail compared with controls unexposed to MT. Histopathology analyses showed an increase in the sperm production at the higher MT concentrations of 0.1 and 1.0 mg/L. Chromatographic analyses of water samples showed that MT concentrations rapidly declined within a 96-h period. These results highlight the importance of giving more support to regulatory authorities, since MT is not registered for use on fish hatcheries in many countries around the world. Wastewater from fish farms discharged into aquatic ecosystems should be monitored for MT residues, since its presence could compromise the reproduction of other native snail species. |
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Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata.Hormônio sintéticoPerformance reprodutivaDesreguladores endócrinosHistopatologiaBiomphalaria GlabrataMethyltestosteroneReproductive performanceEndocrine-disrupting chemicalsHistopathologySynthetic hormones17-α-methyltestosterone (MT) is a synthetic hormone used in fish hatcheries to induce male monosex. Snails hold promise as possible test models to assess chemicals acting on the endocrine system. Biomphalaria glabrata is an aquatic gastropod mollusk (Pulmonata, Planorbidae) that can be easily maintained in aquaria, predisposing the species for use in ecotoxicological testing. This study evaluated the reproductive effects of MT on B. glabrata by examining histological changes and its reproductive performance. Ten snails per group were exposed for 4 weeks to different concentrations of MT (0.01, 0.1, and 1.0 mg/L). The total number of laid eggs, egg mass per group, size of type V oocytes, and production of spermatozoids were determined. Reproduction of B. glabrata was affected by MT. At the lowest concentration (0.01 mg/L), MT caused a statistically significant increase in the number of egg mass per snail compared with controls unexposed to MT. Histopathology analyses showed an increase in the sperm production at the higher MT concentrations of 0.1 and 1.0 mg/L. Chromatographic analyses of water samples showed that MT concentrations rapidly declined within a 96-h period. These results highlight the importance of giving more support to regulatory authorities, since MT is not registered for use on fish hatcheries in many countries around the world. Wastewater from fish farms discharged into aquatic ecosystems should be monitored for MT residues, since its presence could compromise the reproduction of other native snail species.UNB; UNB; EDUARDO CYRINO DE OLIVEIRA FILHO, CPAC; UNB; UNB; UNB.RIVERO-WENDT, C. L. G.BORGES, A. C.OLIVEIRA FILHO, E. C. deMIRANDA-VILELA, A. L.FERREIRA, M. F. N.GRISOLIA, C. K.2014-12-15T11:11:11Z2014-12-15T11:11:11Z2014-12-1520142014-12-15T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 13, n. 1, p. 605-615, 2014.1676-5680http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002535http://dx.doi.org/10.4238/2014.January.28.6enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T01:36:58Zoai:www.alice.cnptia.embrapa.br:doc/1002535Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T01:36:58falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T01:36:58Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata. |
title |
Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata. |
spellingShingle |
Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata. RIVERO-WENDT, C. L. G. Hormônio sintético Performance reprodutiva Desreguladores endócrinos Histopatologia Biomphalaria Glabrata Methyltestosterone Reproductive performance Endocrine-disrupting chemicals Histopathology Synthetic hormones |
title_short |
Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata. |
title_full |
Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata. |
title_fullStr |
Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata. |
title_full_unstemmed |
Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata. |
title_sort |
Effects of 17α-methyltestosterone on the reproduction of the freshwater snail Biomphalaria glabrata. |
author |
RIVERO-WENDT, C. L. G. |
author_facet |
RIVERO-WENDT, C. L. G. BORGES, A. C. OLIVEIRA FILHO, E. C. de MIRANDA-VILELA, A. L. FERREIRA, M. F. N. GRISOLIA, C. K. |
author_role |
author |
author2 |
BORGES, A. C. OLIVEIRA FILHO, E. C. de MIRANDA-VILELA, A. L. FERREIRA, M. F. N. GRISOLIA, C. K. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
UNB; UNB; EDUARDO CYRINO DE OLIVEIRA FILHO, CPAC; UNB; UNB; UNB. |
dc.contributor.author.fl_str_mv |
RIVERO-WENDT, C. L. G. BORGES, A. C. OLIVEIRA FILHO, E. C. de MIRANDA-VILELA, A. L. FERREIRA, M. F. N. GRISOLIA, C. K. |
dc.subject.por.fl_str_mv |
Hormônio sintético Performance reprodutiva Desreguladores endócrinos Histopatologia Biomphalaria Glabrata Methyltestosterone Reproductive performance Endocrine-disrupting chemicals Histopathology Synthetic hormones |
topic |
Hormônio sintético Performance reprodutiva Desreguladores endócrinos Histopatologia Biomphalaria Glabrata Methyltestosterone Reproductive performance Endocrine-disrupting chemicals Histopathology Synthetic hormones |
description |
17-α-methyltestosterone (MT) is a synthetic hormone used in fish hatcheries to induce male monosex. Snails hold promise as possible test models to assess chemicals acting on the endocrine system. Biomphalaria glabrata is an aquatic gastropod mollusk (Pulmonata, Planorbidae) that can be easily maintained in aquaria, predisposing the species for use in ecotoxicological testing. This study evaluated the reproductive effects of MT on B. glabrata by examining histological changes and its reproductive performance. Ten snails per group were exposed for 4 weeks to different concentrations of MT (0.01, 0.1, and 1.0 mg/L). The total number of laid eggs, egg mass per group, size of type V oocytes, and production of spermatozoids were determined. Reproduction of B. glabrata was affected by MT. At the lowest concentration (0.01 mg/L), MT caused a statistically significant increase in the number of egg mass per snail compared with controls unexposed to MT. Histopathology analyses showed an increase in the sperm production at the higher MT concentrations of 0.1 and 1.0 mg/L. Chromatographic analyses of water samples showed that MT concentrations rapidly declined within a 96-h period. These results highlight the importance of giving more support to regulatory authorities, since MT is not registered for use on fish hatcheries in many countries around the world. Wastewater from fish farms discharged into aquatic ecosystems should be monitored for MT residues, since its presence could compromise the reproduction of other native snail species. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-15T11:11:11Z 2014-12-15T11:11:11Z 2014-12-15 2014 2014-12-15T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 13, n. 1, p. 605-615, 2014. 1676-5680 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002535 http://dx.doi.org/10.4238/2014.January.28.6 |
identifier_str_mv |
Genetics and Molecular Research, v. 13, n. 1, p. 605-615, 2014. 1676-5680 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1002535 http://dx.doi.org/10.4238/2014.January.28.6 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503398544900096 |