Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1019634 |
Resumo: | The objective of this work was to isolate and characterize Bacillus thuringiensis from agricultural and other insect breeding sites in Mali. A hundred soil samples were collected from Bamako district, Segou, Sikasso and Timbuktu regions. B. thuringiensis (Bt) was isolated from the samples using a heat-acetate method and the isolates were identified and classified using morphological and biochemical tests. The frequency of B. thuringiensis in soils was noted. The results showed that, 15 out of the 3111 bacterial isolates were putative Bt. thuringiensis. Most isolates produced parasporal crystals. The average Bt index for all the areas sampled was 5.1%; the highest frequency was recorded for Niono in Segou region (11.7) and the lowest for Bozola in Bamako district (0.5). Contrary the known information on the high content and distribution of B. in soils, the agricultural soils of Mali contain few Bt strains, confirmed by the low Bt index obtained. |
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Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali.BacteriaInsetoSoloThe objective of this work was to isolate and characterize Bacillus thuringiensis from agricultural and other insect breeding sites in Mali. A hundred soil samples were collected from Bamako district, Segou, Sikasso and Timbuktu regions. B. thuringiensis (Bt) was isolated from the samples using a heat-acetate method and the isolates were identified and classified using morphological and biochemical tests. The frequency of B. thuringiensis in soils was noted. The results showed that, 15 out of the 3111 bacterial isolates were putative Bt. thuringiensis. Most isolates produced parasporal crystals. The average Bt index for all the areas sampled was 5.1%; the highest frequency was recorded for Niono in Segou region (11.7) and the lowest for Bozola in Bamako district (0.5). Contrary the known information on the high content and distribution of B. in soils, the agricultural soils of Mali contain few Bt strains, confirmed by the low Bt index obtained.FERNANDO HERCOS VALICENTE, CNPMS.KASSOGUÉ, A.MAÏGA, K.TRAORÉ, D.DICKO, A. H.FANÉ, R.GISSOU, T.FARADJI, F. A.VALICENTE, F. H.HAMADOUN, A.BADANA, A. H.2015-07-10T11:11:11Z2015-07-10T11:11:11Z2015-07-1020152017-07-19T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAfrican Journal of Agricultural Research, v. 10, n. 28, p. 2748-2755, 2015.http://www.alice.cnptia.embrapa.br/alice/handle/doc/101963410.5897/AJAR2015.9848porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:30:28Zoai:www.alice.cnptia.embrapa.br:doc/1019634Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:30:28falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:30:28Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali. |
title |
Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali. |
spellingShingle |
Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali. KASSOGUÉ, A. Bacteria Inseto Solo |
title_short |
Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali. |
title_full |
Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali. |
title_fullStr |
Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali. |
title_full_unstemmed |
Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali. |
title_sort |
Isolation and characterization of Bacillus thuringiensis (Ernst Berliner) strains indigenous to agricultural soils of Mali. |
author |
KASSOGUÉ, A. |
author_facet |
KASSOGUÉ, A. MAÏGA, K. TRAORÉ, D. DICKO, A. H. FANÉ, R. GISSOU, T. FARADJI, F. A. VALICENTE, F. H. HAMADOUN, A. BADANA, A. H. |
author_role |
author |
author2 |
MAÏGA, K. TRAORÉ, D. DICKO, A. H. FANÉ, R. GISSOU, T. FARADJI, F. A. VALICENTE, F. H. HAMADOUN, A. BADANA, A. H. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
FERNANDO HERCOS VALICENTE, CNPMS. |
dc.contributor.author.fl_str_mv |
KASSOGUÉ, A. MAÏGA, K. TRAORÉ, D. DICKO, A. H. FANÉ, R. GISSOU, T. FARADJI, F. A. VALICENTE, F. H. HAMADOUN, A. BADANA, A. H. |
dc.subject.por.fl_str_mv |
Bacteria Inseto Solo |
topic |
Bacteria Inseto Solo |
description |
The objective of this work was to isolate and characterize Bacillus thuringiensis from agricultural and other insect breeding sites in Mali. A hundred soil samples were collected from Bamako district, Segou, Sikasso and Timbuktu regions. B. thuringiensis (Bt) was isolated from the samples using a heat-acetate method and the isolates were identified and classified using morphological and biochemical tests. The frequency of B. thuringiensis in soils was noted. The results showed that, 15 out of the 3111 bacterial isolates were putative Bt. thuringiensis. Most isolates produced parasporal crystals. The average Bt index for all the areas sampled was 5.1%; the highest frequency was recorded for Niono in Segou region (11.7) and the lowest for Bozola in Bamako district (0.5). Contrary the known information on the high content and distribution of B. in soils, the agricultural soils of Mali contain few Bt strains, confirmed by the low Bt index obtained. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-10T11:11:11Z 2015-07-10T11:11:11Z 2015-07-10 2015 2017-07-19T11:11:11Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
African Journal of Agricultural Research, v. 10, n. 28, p. 2748-2755, 2015. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1019634 10.5897/AJAR2015.9848 |
identifier_str_mv |
African Journal of Agricultural Research, v. 10, n. 28, p. 2748-2755, 2015. 10.5897/AJAR2015.9848 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1019634 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503437203800064 |