Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.

Detalhes bibliográficos
Autor(a) principal: CARDOSO, T. F.
Data de Publicação: 2021
Outros Autores: COUTINHO, L. L., BRUSCADIN, J. J., DINIZ, W. J. da S., PETRINI, J., ANDRADE, B. G. N., OLIVEIRA, P. S. N. de, POLETI, M. D., CESAR, A. S. M., SILVEIRA, J. C. DA, CHIARATTI, M. R., ZERLOTINI NETO, A., MOURÃO, G. B., REGITANO, L. C. de A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131655
https://doi.org/10.3390/genes12010067
Resumo: MicroRNAs (miRNAs) are key regulators of gene expression, potentially affecting several biological processes, whose function can be altered by sequence variation. Hence, the integration of single nucleotide polymorphisms (SNP) and miRNAs can explain individual differences in economic traits. To provide new insights into the effects of SNPs on miRNAs and their related target genes, we carried out a multi-omic analysis to identify SNPs in miRNA mature sequences (miR-SNPs) associated with fatty acid (FA) composition in the Nelore cattle. As a result, we identified 3 miR-SNPs in different miRNAs (bta-miR-2419-3p, bta-miR-193a-2, and bta-miR-1291) significantly associated with FA traits (p-value < 0.02, Bonferroni corrected). Among these, the rs110817643C>T, located in the seed sequence of the bta-miR-1291, was associated with different ?6 FAs, polyunsaturated FA, and polyunsaturated:saturated FA ratios. Concerning the other two miR-SNPs, the rs43400521T>C (located in the bta-miR-2419-3p) was associated with C12:0 and C18:1 cis-11 FA, whereas the rs516857374A>G (located in the bta-miR-193a-2) was associated with C18:3 ?6 and ratio of ?6/?3 traits. Additionally, to identify potential biomarkers for FA composition, we described target genes affected by these miR-SNPs at the mRNA or protein level. Our multi-omics analysis outlines the effects of genetic polymorphism on miRNA, and it highlights miR-SNPs and target candidate genes that control beef fatty acid composition.
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spelling Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.Análise multiômicaExpressão gênicaPolimorfismo de nucleotídeo únicoAssociation analysisMiRNAsBos IndicusBeef qualityPolymorphismGene expressionSingle nucleotide polymorphismMicroRNAs (miRNAs) are key regulators of gene expression, potentially affecting several biological processes, whose function can be altered by sequence variation. Hence, the integration of single nucleotide polymorphisms (SNP) and miRNAs can explain individual differences in economic traits. To provide new insights into the effects of SNPs on miRNAs and their related target genes, we carried out a multi-omic analysis to identify SNPs in miRNA mature sequences (miR-SNPs) associated with fatty acid (FA) composition in the Nelore cattle. As a result, we identified 3 miR-SNPs in different miRNAs (bta-miR-2419-3p, bta-miR-193a-2, and bta-miR-1291) significantly associated with FA traits (p-value < 0.02, Bonferroni corrected). Among these, the rs110817643C>T, located in the seed sequence of the bta-miR-1291, was associated with different ?6 FAs, polyunsaturated FA, and polyunsaturated:saturated FA ratios. Concerning the other two miR-SNPs, the rs43400521T>C (located in the bta-miR-2419-3p) was associated with C12:0 and C18:1 cis-11 FA, whereas the rs516857374A>G (located in the bta-miR-193a-2) was associated with C18:3 ?6 and ratio of ?6/?3 traits. Additionally, to identify potential biomarkers for FA composition, we described target genes affected by these miR-SNPs at the mRNA or protein level. Our multi-omics analysis outlines the effects of genetic polymorphism on miRNA, and it highlights miR-SNPs and target candidate genes that control beef fatty acid composition.Article 67. Na publicação: Adhemar Zerlotini.TAINÃ FIGUEIREDO CARDOSO; LUIZ LEHMANN COUTINHO, ESALQ/USP; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, North Dakota State University; JULIANA PETRINI, ESALQ/USP; BRUNO GABRIEL NASCIMENTO ANDRADE; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; MIRELE DAIANA POLETI, USP; ALINE SILVA MELLO CESAR, ESALQ/USP; JULIANO COELHO DA SILVEIRA, USP; MARCOS ROBERTO CHIARATTI, UFSCAR; ADHEMAR ZERLOTINI NETO, CNPTIA; GERSON BARRETO MOURÃO, ESALQ/USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.CARDOSO, T. F.COUTINHO, L. L.BRUSCADIN, J. J.DINIZ, W. J. da S.PETRINI, J.ANDRADE, B. G. N.OLIVEIRA, P. S. N. dePOLETI, M. D.CESAR, A. S. M.SILVEIRA, J. C. DACHIARATTI, M. R.ZERLOTINI NETO, A.MOURÃO, G. B.REGITANO, L. C. de A.2021-05-04T15:30:54Z2021-05-04T15:30:54Z2021-05-042021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenes, v. 12, n. 1, p. 1-18, Jan. 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131655https://doi.org/10.3390/genes12010067enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-05-04T15:31:04Zoai:www.alice.cnptia.embrapa.br:doc/1131655Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-05-04T15:31:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-05-04T15:31:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
title Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
spellingShingle Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
CARDOSO, T. F.
Análise multiômica
Expressão gênica
Polimorfismo de nucleotídeo único
Association analysis
MiRNAs
Bos Indicus
Beef quality
Polymorphism
Gene expression
Single nucleotide polymorphism
title_short Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
title_full Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
title_fullStr Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
title_full_unstemmed Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
title_sort Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
author CARDOSO, T. F.
author_facet CARDOSO, T. F.
COUTINHO, L. L.
BRUSCADIN, J. J.
DINIZ, W. J. da S.
PETRINI, J.
ANDRADE, B. G. N.
OLIVEIRA, P. S. N. de
POLETI, M. D.
CESAR, A. S. M.
SILVEIRA, J. C. DA
CHIARATTI, M. R.
ZERLOTINI NETO, A.
MOURÃO, G. B.
REGITANO, L. C. de A.
author_role author
author2 COUTINHO, L. L.
BRUSCADIN, J. J.
DINIZ, W. J. da S.
PETRINI, J.
ANDRADE, B. G. N.
OLIVEIRA, P. S. N. de
POLETI, M. D.
CESAR, A. S. M.
SILVEIRA, J. C. DA
CHIARATTI, M. R.
ZERLOTINI NETO, A.
MOURÃO, G. B.
REGITANO, L. C. de A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv TAINÃ FIGUEIREDO CARDOSO; LUIZ LEHMANN COUTINHO, ESALQ/USP; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, North Dakota State University; JULIANA PETRINI, ESALQ/USP; BRUNO GABRIEL NASCIMENTO ANDRADE; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; MIRELE DAIANA POLETI, USP; ALINE SILVA MELLO CESAR, ESALQ/USP; JULIANO COELHO DA SILVEIRA, USP; MARCOS ROBERTO CHIARATTI, UFSCAR; ADHEMAR ZERLOTINI NETO, CNPTIA; GERSON BARRETO MOURÃO, ESALQ/USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
dc.contributor.author.fl_str_mv CARDOSO, T. F.
COUTINHO, L. L.
BRUSCADIN, J. J.
DINIZ, W. J. da S.
PETRINI, J.
ANDRADE, B. G. N.
OLIVEIRA, P. S. N. de
POLETI, M. D.
CESAR, A. S. M.
SILVEIRA, J. C. DA
CHIARATTI, M. R.
ZERLOTINI NETO, A.
MOURÃO, G. B.
REGITANO, L. C. de A.
dc.subject.por.fl_str_mv Análise multiômica
Expressão gênica
Polimorfismo de nucleotídeo único
Association analysis
MiRNAs
Bos Indicus
Beef quality
Polymorphism
Gene expression
Single nucleotide polymorphism
topic Análise multiômica
Expressão gênica
Polimorfismo de nucleotídeo único
Association analysis
MiRNAs
Bos Indicus
Beef quality
Polymorphism
Gene expression
Single nucleotide polymorphism
description MicroRNAs (miRNAs) are key regulators of gene expression, potentially affecting several biological processes, whose function can be altered by sequence variation. Hence, the integration of single nucleotide polymorphisms (SNP) and miRNAs can explain individual differences in economic traits. To provide new insights into the effects of SNPs on miRNAs and their related target genes, we carried out a multi-omic analysis to identify SNPs in miRNA mature sequences (miR-SNPs) associated with fatty acid (FA) composition in the Nelore cattle. As a result, we identified 3 miR-SNPs in different miRNAs (bta-miR-2419-3p, bta-miR-193a-2, and bta-miR-1291) significantly associated with FA traits (p-value < 0.02, Bonferroni corrected). Among these, the rs110817643C>T, located in the seed sequence of the bta-miR-1291, was associated with different ?6 FAs, polyunsaturated FA, and polyunsaturated:saturated FA ratios. Concerning the other two miR-SNPs, the rs43400521T>C (located in the bta-miR-2419-3p) was associated with C12:0 and C18:1 cis-11 FA, whereas the rs516857374A>G (located in the bta-miR-193a-2) was associated with C18:3 ?6 and ratio of ?6/?3 traits. Additionally, to identify potential biomarkers for FA composition, we described target genes affected by these miR-SNPs at the mRNA or protein level. Our multi-omics analysis outlines the effects of genetic polymorphism on miRNA, and it highlights miR-SNPs and target candidate genes that control beef fatty acid composition.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-04T15:30:54Z
2021-05-04T15:30:54Z
2021-05-04
2021
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Genes, v. 12, n. 1, p. 1-18, Jan. 2021.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131655
https://doi.org/10.3390/genes12010067
identifier_str_mv Genes, v. 12, n. 1, p. 1-18, Jan. 2021.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131655
https://doi.org/10.3390/genes12010067
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
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repository.mail.fl_str_mv cg-riaa@embrapa.br
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