Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131655 https://doi.org/10.3390/genes12010067 |
Resumo: | MicroRNAs (miRNAs) are key regulators of gene expression, potentially affecting several biological processes, whose function can be altered by sequence variation. Hence, the integration of single nucleotide polymorphisms (SNP) and miRNAs can explain individual differences in economic traits. To provide new insights into the effects of SNPs on miRNAs and their related target genes, we carried out a multi-omic analysis to identify SNPs in miRNA mature sequences (miR-SNPs) associated with fatty acid (FA) composition in the Nelore cattle. As a result, we identified 3 miR-SNPs in different miRNAs (bta-miR-2419-3p, bta-miR-193a-2, and bta-miR-1291) significantly associated with FA traits (p-value < 0.02, Bonferroni corrected). Among these, the rs110817643C>T, located in the seed sequence of the bta-miR-1291, was associated with different ?6 FAs, polyunsaturated FA, and polyunsaturated:saturated FA ratios. Concerning the other two miR-SNPs, the rs43400521T>C (located in the bta-miR-2419-3p) was associated with C12:0 and C18:1 cis-11 FA, whereas the rs516857374A>G (located in the bta-miR-193a-2) was associated with C18:3 ?6 and ratio of ?6/?3 traits. Additionally, to identify potential biomarkers for FA composition, we described target genes affected by these miR-SNPs at the mRNA or protein level. Our multi-omics analysis outlines the effects of genetic polymorphism on miRNA, and it highlights miR-SNPs and target candidate genes that control beef fatty acid composition. |
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Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle.Análise multiômicaExpressão gênicaPolimorfismo de nucleotídeo únicoAssociation analysisMiRNAsBos IndicusBeef qualityPolymorphismGene expressionSingle nucleotide polymorphismMicroRNAs (miRNAs) are key regulators of gene expression, potentially affecting several biological processes, whose function can be altered by sequence variation. Hence, the integration of single nucleotide polymorphisms (SNP) and miRNAs can explain individual differences in economic traits. To provide new insights into the effects of SNPs on miRNAs and their related target genes, we carried out a multi-omic analysis to identify SNPs in miRNA mature sequences (miR-SNPs) associated with fatty acid (FA) composition in the Nelore cattle. As a result, we identified 3 miR-SNPs in different miRNAs (bta-miR-2419-3p, bta-miR-193a-2, and bta-miR-1291) significantly associated with FA traits (p-value < 0.02, Bonferroni corrected). Among these, the rs110817643C>T, located in the seed sequence of the bta-miR-1291, was associated with different ?6 FAs, polyunsaturated FA, and polyunsaturated:saturated FA ratios. Concerning the other two miR-SNPs, the rs43400521T>C (located in the bta-miR-2419-3p) was associated with C12:0 and C18:1 cis-11 FA, whereas the rs516857374A>G (located in the bta-miR-193a-2) was associated with C18:3 ?6 and ratio of ?6/?3 traits. Additionally, to identify potential biomarkers for FA composition, we described target genes affected by these miR-SNPs at the mRNA or protein level. Our multi-omics analysis outlines the effects of genetic polymorphism on miRNA, and it highlights miR-SNPs and target candidate genes that control beef fatty acid composition.Article 67. Na publicação: Adhemar Zerlotini.TAINÃ FIGUEIREDO CARDOSO; LUIZ LEHMANN COUTINHO, ESALQ/USP; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, North Dakota State University; JULIANA PETRINI, ESALQ/USP; BRUNO GABRIEL NASCIMENTO ANDRADE; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; MIRELE DAIANA POLETI, USP; ALINE SILVA MELLO CESAR, ESALQ/USP; JULIANO COELHO DA SILVEIRA, USP; MARCOS ROBERTO CHIARATTI, UFSCAR; ADHEMAR ZERLOTINI NETO, CNPTIA; GERSON BARRETO MOURÃO, ESALQ/USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.CARDOSO, T. F.COUTINHO, L. L.BRUSCADIN, J. J.DINIZ, W. J. da S.PETRINI, J.ANDRADE, B. G. N.OLIVEIRA, P. S. N. dePOLETI, M. D.CESAR, A. S. M.SILVEIRA, J. C. DACHIARATTI, M. R.ZERLOTINI NETO, A.MOURÃO, G. B.REGITANO, L. C. de A.2021-05-04T15:30:54Z2021-05-04T15:30:54Z2021-05-042021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenes, v. 12, n. 1, p. 1-18, Jan. 2021.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131655https://doi.org/10.3390/genes12010067enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-05-04T15:31:04Zoai:www.alice.cnptia.embrapa.br:doc/1131655Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-05-04T15:31:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-05-04T15:31:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle. |
title |
Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle. |
spellingShingle |
Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle. CARDOSO, T. F. Análise multiômica Expressão gênica Polimorfismo de nucleotídeo único Association analysis MiRNAs Bos Indicus Beef quality Polymorphism Gene expression Single nucleotide polymorphism |
title_short |
Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle. |
title_full |
Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle. |
title_fullStr |
Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle. |
title_full_unstemmed |
Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle. |
title_sort |
Multi-omics approach reveals miR-SNPs affecting muscle fatty acids profile in Nelore cattle. |
author |
CARDOSO, T. F. |
author_facet |
CARDOSO, T. F. COUTINHO, L. L. BRUSCADIN, J. J. DINIZ, W. J. da S. PETRINI, J. ANDRADE, B. G. N. OLIVEIRA, P. S. N. de POLETI, M. D. CESAR, A. S. M. SILVEIRA, J. C. DA CHIARATTI, M. R. ZERLOTINI NETO, A. MOURÃO, G. B. REGITANO, L. C. de A. |
author_role |
author |
author2 |
COUTINHO, L. L. BRUSCADIN, J. J. DINIZ, W. J. da S. PETRINI, J. ANDRADE, B. G. N. OLIVEIRA, P. S. N. de POLETI, M. D. CESAR, A. S. M. SILVEIRA, J. C. DA CHIARATTI, M. R. ZERLOTINI NETO, A. MOURÃO, G. B. REGITANO, L. C. de A. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
TAINÃ FIGUEIREDO CARDOSO; LUIZ LEHMANN COUTINHO, ESALQ/USP; JENNIFER JESSICA BRUSCADIN, UFSCAR; WELLISON JARLES DA SILVA DINIZ, North Dakota State University; JULIANA PETRINI, ESALQ/USP; BRUNO GABRIEL NASCIMENTO ANDRADE; PRISCILA SILVA NEUBERN DE OLIVEIRA, UFSCAR; MIRELE DAIANA POLETI, USP; ALINE SILVA MELLO CESAR, ESALQ/USP; JULIANO COELHO DA SILVEIRA, USP; MARCOS ROBERTO CHIARATTI, UFSCAR; ADHEMAR ZERLOTINI NETO, CNPTIA; GERSON BARRETO MOURÃO, ESALQ/USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE. |
dc.contributor.author.fl_str_mv |
CARDOSO, T. F. COUTINHO, L. L. BRUSCADIN, J. J. DINIZ, W. J. da S. PETRINI, J. ANDRADE, B. G. N. OLIVEIRA, P. S. N. de POLETI, M. D. CESAR, A. S. M. SILVEIRA, J. C. DA CHIARATTI, M. R. ZERLOTINI NETO, A. MOURÃO, G. B. REGITANO, L. C. de A. |
dc.subject.por.fl_str_mv |
Análise multiômica Expressão gênica Polimorfismo de nucleotídeo único Association analysis MiRNAs Bos Indicus Beef quality Polymorphism Gene expression Single nucleotide polymorphism |
topic |
Análise multiômica Expressão gênica Polimorfismo de nucleotídeo único Association analysis MiRNAs Bos Indicus Beef quality Polymorphism Gene expression Single nucleotide polymorphism |
description |
MicroRNAs (miRNAs) are key regulators of gene expression, potentially affecting several biological processes, whose function can be altered by sequence variation. Hence, the integration of single nucleotide polymorphisms (SNP) and miRNAs can explain individual differences in economic traits. To provide new insights into the effects of SNPs on miRNAs and their related target genes, we carried out a multi-omic analysis to identify SNPs in miRNA mature sequences (miR-SNPs) associated with fatty acid (FA) composition in the Nelore cattle. As a result, we identified 3 miR-SNPs in different miRNAs (bta-miR-2419-3p, bta-miR-193a-2, and bta-miR-1291) significantly associated with FA traits (p-value < 0.02, Bonferroni corrected). Among these, the rs110817643C>T, located in the seed sequence of the bta-miR-1291, was associated with different ?6 FAs, polyunsaturated FA, and polyunsaturated:saturated FA ratios. Concerning the other two miR-SNPs, the rs43400521T>C (located in the bta-miR-2419-3p) was associated with C12:0 and C18:1 cis-11 FA, whereas the rs516857374A>G (located in the bta-miR-193a-2) was associated with C18:3 ?6 and ratio of ?6/?3 traits. Additionally, to identify potential biomarkers for FA composition, we described target genes affected by these miR-SNPs at the mRNA or protein level. Our multi-omics analysis outlines the effects of genetic polymorphism on miRNA, and it highlights miR-SNPs and target candidate genes that control beef fatty acid composition. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-04T15:30:54Z 2021-05-04T15:30:54Z 2021-05-04 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genes, v. 12, n. 1, p. 1-18, Jan. 2021. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131655 https://doi.org/10.3390/genes12010067 |
identifier_str_mv |
Genes, v. 12, n. 1, p. 1-18, Jan. 2021. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1131655 https://doi.org/10.3390/genes12010067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503504918740992 |