Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822).
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160896 https://doi.org/10.3390/fishes9010024 |
Resumo: | The pirarucu, Arapaima gigas, is an emblematic endangered Amazon osteoglossid with high potential for aquaculture development. In this study, we report the in vivo collection of semen from pirarucu for the first-time and a complete description of the spermatozoa ultrastructure using scanning and transmission electron microscopy (SEM and TEM). The spermatozoon of pirarucu is biflagellate with no acrosomal cell, comprising a spherical head (8.26 ± 2.19 μm2), a short intermediate piece region and two long flagella (68.34 ± 5.69 μm). The nucleus is spherical and electron-dense, and at its base, there are two nuclear fossae where the centrioles are found. The anterior part of the flagellum is positioned at the lateral base of the nucleus, forming an intermediate piece. Lateral to the intermediate piece, an abundant number of mitochondria are present and occupy most of the cytoplasmic volume. The two flagella were found with a 9 + 2 axonemal structure (nine peripheral doublets and two central microtubules). The central microtubules are aligned with two side fins formed laterally by the axoneme plasma membrane. Side fins were observed from the anterior region to the posterior end. The results show that the spermatozoa of A. gigas are biflagellate, mitochondria-rich and robust, corroborating the external fertilization characteristic of the species. The novel description of sperm morphology presented herein will contribute to advancing the knowledge of and aiding future research on the reproductive biology of this species. |
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Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822).PirarucuPeixeReprodução AnimalMorfologia AnimalEspermatozóideSpermatozoaOsteoglossidaeArapaima gigasReproductionUltrastructureThe pirarucu, Arapaima gigas, is an emblematic endangered Amazon osteoglossid with high potential for aquaculture development. In this study, we report the in vivo collection of semen from pirarucu for the first-time and a complete description of the spermatozoa ultrastructure using scanning and transmission electron microscopy (SEM and TEM). The spermatozoon of pirarucu is biflagellate with no acrosomal cell, comprising a spherical head (8.26 ± 2.19 μm2), a short intermediate piece region and two long flagella (68.34 ± 5.69 μm). The nucleus is spherical and electron-dense, and at its base, there are two nuclear fossae where the centrioles are found. The anterior part of the flagellum is positioned at the lateral base of the nucleus, forming an intermediate piece. Lateral to the intermediate piece, an abundant number of mitochondria are present and occupy most of the cytoplasmic volume. The two flagella were found with a 9 + 2 axonemal structure (nine peripheral doublets and two central microtubules). The central microtubules are aligned with two side fins formed laterally by the axoneme plasma membrane. Side fins were observed from the anterior region to the posterior end. The results show that the spermatozoa of A. gigas are biflagellate, mitochondria-rich and robust, corroborating the external fertilization characteristic of the species. The novel description of sperm morphology presented herein will contribute to advancing the knowledge of and aiding future research on the reproductive biology of this species.LUCAS SIMON TORATI, CNPASAJULIA T. LOPES, IFTO, Pedro Afonso-TOADRIANA FERREIRA LIMA, CNPASAVELMURUGU PUVANENDRAN, CENTER FOR MARINE AQUACULTURE & PRODUCTION BIOLOGY, NorwaySERGIO R. BATLOUNI, UNESP, Jaboticabal-SPLUCIANA NAKAGHI GANECO KIRSCHNIK, CNPASA.TORATI, L. S.LOPES, J. T.LIMA, A. F.PUVANENDRAN, V.BATLOUNI, S. R.KIRSCHNIK, L. N. G.2024-01-17T11:32:32Z2024-01-17T11:32:32Z2024-01-172024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFishes, v. 9, 24, 2024.2410-3888http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160896https://doi.org/10.3390/fishes9010024enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-01-17T11:32:32Zoai:www.alice.cnptia.embrapa.br:doc/1160896Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-01-17T11:32:32falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-01-17T11:32:32Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). |
title |
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). |
spellingShingle |
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). TORATI, L. S. Pirarucu Peixe Reprodução Animal Morfologia Animal Espermatozóide Spermatozoa Osteoglossidae Arapaima gigas Reproduction Ultrastructure |
title_short |
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). |
title_full |
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). |
title_fullStr |
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). |
title_full_unstemmed |
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). |
title_sort |
Feasibility of In Vivo semen collection and description of the morphology and ultrastructure of the spermatozoa of Arapaima gigas (Schinz, 1822). |
author |
TORATI, L. S. |
author_facet |
TORATI, L. S. LOPES, J. T. LIMA, A. F. PUVANENDRAN, V. BATLOUNI, S. R. KIRSCHNIK, L. N. G. |
author_role |
author |
author2 |
LOPES, J. T. LIMA, A. F. PUVANENDRAN, V. BATLOUNI, S. R. KIRSCHNIK, L. N. G. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
LUCAS SIMON TORATI, CNPASA JULIA T. LOPES, IFTO, Pedro Afonso-TO ADRIANA FERREIRA LIMA, CNPASA VELMURUGU PUVANENDRAN, CENTER FOR MARINE AQUACULTURE & PRODUCTION BIOLOGY, Norway SERGIO R. BATLOUNI, UNESP, Jaboticabal-SP LUCIANA NAKAGHI GANECO KIRSCHNIK, CNPASA. |
dc.contributor.author.fl_str_mv |
TORATI, L. S. LOPES, J. T. LIMA, A. F. PUVANENDRAN, V. BATLOUNI, S. R. KIRSCHNIK, L. N. G. |
dc.subject.por.fl_str_mv |
Pirarucu Peixe Reprodução Animal Morfologia Animal Espermatozóide Spermatozoa Osteoglossidae Arapaima gigas Reproduction Ultrastructure |
topic |
Pirarucu Peixe Reprodução Animal Morfologia Animal Espermatozóide Spermatozoa Osteoglossidae Arapaima gigas Reproduction Ultrastructure |
description |
The pirarucu, Arapaima gigas, is an emblematic endangered Amazon osteoglossid with high potential for aquaculture development. In this study, we report the in vivo collection of semen from pirarucu for the first-time and a complete description of the spermatozoa ultrastructure using scanning and transmission electron microscopy (SEM and TEM). The spermatozoon of pirarucu is biflagellate with no acrosomal cell, comprising a spherical head (8.26 ± 2.19 μm2), a short intermediate piece region and two long flagella (68.34 ± 5.69 μm). The nucleus is spherical and electron-dense, and at its base, there are two nuclear fossae where the centrioles are found. The anterior part of the flagellum is positioned at the lateral base of the nucleus, forming an intermediate piece. Lateral to the intermediate piece, an abundant number of mitochondria are present and occupy most of the cytoplasmic volume. The two flagella were found with a 9 + 2 axonemal structure (nine peripheral doublets and two central microtubules). The central microtubules are aligned with two side fins formed laterally by the axoneme plasma membrane. Side fins were observed from the anterior region to the posterior end. The results show that the spermatozoa of A. gigas are biflagellate, mitochondria-rich and robust, corroborating the external fertilization characteristic of the species. The novel description of sperm morphology presented herein will contribute to advancing the knowledge of and aiding future research on the reproductive biology of this species. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-01-17T11:32:32Z 2024-01-17T11:32:32Z 2024-01-17 2024 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Fishes, v. 9, 24, 2024. 2410-3888 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160896 https://doi.org/10.3390/fishes9010024 |
identifier_str_mv |
Fishes, v. 9, 24, 2024. 2410-3888 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1160896 https://doi.org/10.3390/fishes9010024 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503556050452480 |