Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note.
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112332 |
Resumo: | Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. |
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Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note.Melhoramento VegetalBreeding and Genetic ImprovementPlant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint.gmr18394CLAUDIA FORTES FERREIRA, CNPMF; D. L. GUTIERREZ, INIA; J. F. KREUZE, CIP; M. L. ISKRA-CARUANA, CIRAD; M. CHABANNES, CIRAD; A. C. O. BARBOSA, UFRB; T. A. SANTOS, UFRB; A. G. S. SILVA, UFRB; R.M.F. SANTOS, UEFS; EDSON PERITO AMORIM, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ONILDO NUNES DE JESUS, CNPMF.FERREIRA, C. F.GUTIERREZ, D. L.KREUZE, J. F.ISKRA-CARUANA, M. L.CHABANNES, M.BARBOSA, A. C. O.SANTOS, T. A.SILVA, A. G. S.SANTOS, R. M. F.AMORIM, E. P.OLIVEIRA, S. A. S. deJESUS, O. N. de2019-12-07T00:33:34Z2019-12-07T00:33:34Z2019-09-1920192019-12-07T00:33:34Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenetics and Molecular Research, v. 18, n.3, 2019.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112332enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-12-07T00:33:45Zoai:www.alice.cnptia.embrapa.br:doc/1112332Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-12-07T00:33:45falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-12-07T00:33:45Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
title |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
spellingShingle |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. FERREIRA, C. F. Melhoramento Vegetal Breeding and Genetic Improvement |
title_short |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
title_full |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
title_fullStr |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
title_full_unstemmed |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
title_sort |
Rapid plant DNA and RNA extraction protocol using a bench drill Brief Note. |
author |
FERREIRA, C. F. |
author_facet |
FERREIRA, C. F. GUTIERREZ, D. L. KREUZE, J. F. ISKRA-CARUANA, M. L. CHABANNES, M. BARBOSA, A. C. O. SANTOS, T. A. SILVA, A. G. S. SANTOS, R. M. F. AMORIM, E. P. OLIVEIRA, S. A. S. de JESUS, O. N. de |
author_role |
author |
author2 |
GUTIERREZ, D. L. KREUZE, J. F. ISKRA-CARUANA, M. L. CHABANNES, M. BARBOSA, A. C. O. SANTOS, T. A. SILVA, A. G. S. SANTOS, R. M. F. AMORIM, E. P. OLIVEIRA, S. A. S. de JESUS, O. N. de |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
CLAUDIA FORTES FERREIRA, CNPMF; D. L. GUTIERREZ, INIA; J. F. KREUZE, CIP; M. L. ISKRA-CARUANA, CIRAD; M. CHABANNES, CIRAD; A. C. O. BARBOSA, UFRB; T. A. SANTOS, UFRB; A. G. S. SILVA, UFRB; R.M.F. SANTOS, UEFS; EDSON PERITO AMORIM, CNPMF; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; ONILDO NUNES DE JESUS, CNPMF. |
dc.contributor.author.fl_str_mv |
FERREIRA, C. F. GUTIERREZ, D. L. KREUZE, J. F. ISKRA-CARUANA, M. L. CHABANNES, M. BARBOSA, A. C. O. SANTOS, T. A. SILVA, A. G. S. SANTOS, R. M. F. AMORIM, E. P. OLIVEIRA, S. A. S. de JESUS, O. N. de |
dc.subject.por.fl_str_mv |
Melhoramento Vegetal Breeding and Genetic Improvement |
topic |
Melhoramento Vegetal Breeding and Genetic Improvement |
description |
Plant DNA and RNA extraction methods are well established, with a wide range of protocols, depending on the purposes of each laboratory/research. Nowadays, quick, inexpensive and easy plant DNA and RNA extraction methods are highly sought after. We developed an optimized protocol for plant DNA and RNA extraction that uses an inexpensive bench drill and plastic bags and does not require liquid nitrogen. DNA from leaves and RNA from leaves and roots of banana, pineapple, citrus, papaya, passion fruit and cassava, were extracted using a basic cetyltrimethylammonium bromide method. Both nucleic acids were quantified and evaluated for quality based on agarose gel electrophoresis. The DNA and RNA extractions were successful for all species, and RNA quality in pellets was maintained after storage at room temperature for three weeks. This protocol can reduce costs considerably in laboratories with ongoing routine activities of DNA and RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic acids. This is especially relevant for many laboratories in developing countries where the cost and availability of liquid nitrogen may be a constraint. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-07T00:33:34Z 2019-12-07T00:33:34Z 2019-09-19 2019 2019-12-07T00:33:34Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Genetics and Molecular Research, v. 18, n.3, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112332 |
identifier_str_mv |
Genetics and Molecular Research, v. 18, n.3, 2019. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1112332 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503485452976128 |