Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.

Detalhes bibliográficos
Autor(a) principal: VIGNA, B. B. Z.
Data de Publicação: 2016
Outros Autores: SANTOS, J. C. S., JUNGMANN, L., VALLE, C. B. do, MOLLINARI, M., PASTINA, M. M., PAGLIARINI, M. S., GARCIA, A. A. F., SOUZA, A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057798
Resumo: The African species Urochloa humidicola (Rendle) Morrone & Zuloaga (syn. Brachiaria humidicola (Rendle) Schweick.) is an important perennial forage grass found throughout the tropics. This species is polyploid, ranging from tetra to nonaploid, and apomictic, which makes genetic studies challenging; therefore, the number of currently available genetic resources is limited. The genomic architecture and evolution of U. humidicola and the molecular markers linked to apomixis were investigated in a full-sib F1 population obtained by crossing the sexual accession H031 and the apomictic cultivar U. humidicola cv. BRS Tupi, both of which are hexaploid. A simple sequence repeat (SSR)-based linkage map was constructed for the species from 102 polymorphic and specific SSR markers based on simplex and double-simplex markers. The map consisted of 49 linkage groups (LGs) and had a total length of 1702.82 cM, with 89 microsatellite loci and an average map density of 10.6 cM. Eight homology groups (HGs) were formed, comprising 22 LGs, and the other LGs remained ungrouped. The locus that controls apospory (apo-locus) was mapped in LG02 and was located 19.4 cM from the locus Bh027.c.D2. In the cytological analyses of some hybrids, bi- to hexavalents at diakinesis were observed, as well as two nucleoli in some meiocytes, smaller chromosomes with preferential allocation within the first metaphase plate and asynchronous chromosome migration to the poles during anaphase. The linkage map and the meiocyte analyses confirm previous reports of hybridization and suggest an allopolyploid origin of the hexaploid U. humidicola. This is the first linkage map of an Urochloa species, and it will be useful for future quantitative trait locus (QTL) analysis after saturation of the map and for genome assembly and evolutionary studies in Urochloa spp. Moreover, the results of the apomixis mapping are consistent with previous reports and confirm the need for additional studies to search for a co-segregating marker.
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spelling Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.Mapeamento genéticoGenePlanta forrageiraThe African species Urochloa humidicola (Rendle) Morrone & Zuloaga (syn. Brachiaria humidicola (Rendle) Schweick.) is an important perennial forage grass found throughout the tropics. This species is polyploid, ranging from tetra to nonaploid, and apomictic, which makes genetic studies challenging; therefore, the number of currently available genetic resources is limited. The genomic architecture and evolution of U. humidicola and the molecular markers linked to apomixis were investigated in a full-sib F1 population obtained by crossing the sexual accession H031 and the apomictic cultivar U. humidicola cv. BRS Tupi, both of which are hexaploid. A simple sequence repeat (SSR)-based linkage map was constructed for the species from 102 polymorphic and specific SSR markers based on simplex and double-simplex markers. The map consisted of 49 linkage groups (LGs) and had a total length of 1702.82 cM, with 89 microsatellite loci and an average map density of 10.6 cM. Eight homology groups (HGs) were formed, comprising 22 LGs, and the other LGs remained ungrouped. The locus that controls apospory (apo-locus) was mapped in LG02 and was located 19.4 cM from the locus Bh027.c.D2. In the cytological analyses of some hybrids, bi- to hexavalents at diakinesis were observed, as well as two nucleoli in some meiocytes, smaller chromosomes with preferential allocation within the first metaphase plate and asynchronous chromosome migration to the poles during anaphase. The linkage map and the meiocyte analyses confirm previous reports of hybridization and suggest an allopolyploid origin of the hexaploid U. humidicola. This is the first linkage map of an Urochloa species, and it will be useful for future quantitative trait locus (QTL) analysis after saturation of the map and for genome assembly and evolutionary studies in Urochloa spp. Moreover, the results of the apomixis mapping are consistent with previous reports and confirm the need for additional studies to search for a co-segregating marker.Bianca B. Z. Vigna, UNICAMP; Jean C. S. Santos, UNICAMP; LETICIA JUNGMANN CANCADO, CNPAE; CACILDA BORGES DO VALLE, CNPGC; Marcelo Mollinari, ESALQ; MARIA MARTA PASTINA, CNPMS; Maria Suely Pagliarini, Universidade Estadual de Maringá; Antonio A. F. Garcia, ESALQ; Anete P. Souza, UNICAMP.VIGNA, B. B. Z.SANTOS, J. C. S.JUNGMANN, L.VALLE, C. B. doMOLLINARI, M.PASTINA, M. M.PAGLIARINI, M. S.GARCIA, A. A. F.SOUZA, A. P.2016-12-02T11:11:11Z2016-12-02T11:11:11Z2016-12-0220162016-12-02T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlos One, San Francisco, v. 11, n. 4, p. 1-23, Apr. 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/105779810.1371/ journal.pone.0153764enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:52:11Zoai:www.alice.cnptia.embrapa.br:doc/1057798Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:52:11Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.
title Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.
spellingShingle Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.
VIGNA, B. B. Z.
Mapeamento genético
Gene
Planta forrageira
title_short Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.
title_full Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.
title_fullStr Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.
title_full_unstemmed Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.
title_sort Evidence of Allopolyploidy in Urochloa humidicola based on cytological analysis and genetic linkage mapping.
author VIGNA, B. B. Z.
author_facet VIGNA, B. B. Z.
SANTOS, J. C. S.
JUNGMANN, L.
VALLE, C. B. do
MOLLINARI, M.
PASTINA, M. M.
PAGLIARINI, M. S.
GARCIA, A. A. F.
SOUZA, A. P.
author_role author
author2 SANTOS, J. C. S.
JUNGMANN, L.
VALLE, C. B. do
MOLLINARI, M.
PASTINA, M. M.
PAGLIARINI, M. S.
GARCIA, A. A. F.
SOUZA, A. P.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Bianca B. Z. Vigna, UNICAMP; Jean C. S. Santos, UNICAMP; LETICIA JUNGMANN CANCADO, CNPAE; CACILDA BORGES DO VALLE, CNPGC; Marcelo Mollinari, ESALQ; MARIA MARTA PASTINA, CNPMS; Maria Suely Pagliarini, Universidade Estadual de Maringá; Antonio A. F. Garcia, ESALQ; Anete P. Souza, UNICAMP.
dc.contributor.author.fl_str_mv VIGNA, B. B. Z.
SANTOS, J. C. S.
JUNGMANN, L.
VALLE, C. B. do
MOLLINARI, M.
PASTINA, M. M.
PAGLIARINI, M. S.
GARCIA, A. A. F.
SOUZA, A. P.
dc.subject.por.fl_str_mv Mapeamento genético
Gene
Planta forrageira
topic Mapeamento genético
Gene
Planta forrageira
description The African species Urochloa humidicola (Rendle) Morrone & Zuloaga (syn. Brachiaria humidicola (Rendle) Schweick.) is an important perennial forage grass found throughout the tropics. This species is polyploid, ranging from tetra to nonaploid, and apomictic, which makes genetic studies challenging; therefore, the number of currently available genetic resources is limited. The genomic architecture and evolution of U. humidicola and the molecular markers linked to apomixis were investigated in a full-sib F1 population obtained by crossing the sexual accession H031 and the apomictic cultivar U. humidicola cv. BRS Tupi, both of which are hexaploid. A simple sequence repeat (SSR)-based linkage map was constructed for the species from 102 polymorphic and specific SSR markers based on simplex and double-simplex markers. The map consisted of 49 linkage groups (LGs) and had a total length of 1702.82 cM, with 89 microsatellite loci and an average map density of 10.6 cM. Eight homology groups (HGs) were formed, comprising 22 LGs, and the other LGs remained ungrouped. The locus that controls apospory (apo-locus) was mapped in LG02 and was located 19.4 cM from the locus Bh027.c.D2. In the cytological analyses of some hybrids, bi- to hexavalents at diakinesis were observed, as well as two nucleoli in some meiocytes, smaller chromosomes with preferential allocation within the first metaphase plate and asynchronous chromosome migration to the poles during anaphase. The linkage map and the meiocyte analyses confirm previous reports of hybridization and suggest an allopolyploid origin of the hexaploid U. humidicola. This is the first linkage map of an Urochloa species, and it will be useful for future quantitative trait locus (QTL) analysis after saturation of the map and for genome assembly and evolutionary studies in Urochloa spp. Moreover, the results of the apomixis mapping are consistent with previous reports and confirm the need for additional studies to search for a co-segregating marker.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-02T11:11:11Z
2016-12-02T11:11:11Z
2016-12-02
2016
2016-12-02T11:11:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plos One, San Francisco, v. 11, n. 4, p. 1-23, Apr. 2016.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057798
10.1371/ journal.pone.0153764
identifier_str_mv Plos One, San Francisco, v. 11, n. 4, p. 1-23, Apr. 2016.
10.1371/ journal.pone.0153764
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057798
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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