Melatonin effect on cryopreserved sperm cells of crioulo stallions.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133434 |
Resumo: | Abstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation. |
id |
EMBR_7edb1d68852efc9ffbb6a8fac227e8d6 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1133434 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
Melatonin effect on cryopreserved sperm cells of crioulo stallions.MelatoninaReprodução AnimalGaranhãoCriopreservaçãoEspermatozóideAnimal reproductionStallionsMelatoninCryopreservationSpermatozoaReactive oxygen speciesAbstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation.CÂNDIDA MALDANER ROMERO TISOT, UPF; RICARDO ZANELLA, UPF; UDESC/Lages; LUCAS MALLMANN LÖF, UPF; UFSM; MARIANA GROKE MARQUES, CNPSA; ERALDO LOURENSO ZANELLA, UPF.ROMERO TISOT, C. M.ZANELLA, R.SOUZA, A. P.LÖF, L. M.SOARES, J. C. M.MARQUES, M. G.ZANELLA, E. L2021-08-11T02:06:52Z2021-08-11T02:06:52Z2021-08-102020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOpen Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020.http://www.alice.cnptia.embrapa.br/alice/handle/doc/113343410.23880/oajvsr-16000202enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-08-11T02:07:00Zoai:www.alice.cnptia.embrapa.br:doc/1133434Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-08-11T02:07Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
title |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
spellingShingle |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. ROMERO TISOT, C. M. Melatonina Reprodução Animal Garanhão Criopreservação Espermatozóide Animal reproduction Stallions Melatonin Cryopreservation Spermatozoa Reactive oxygen species |
title_short |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
title_full |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
title_fullStr |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
title_full_unstemmed |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
title_sort |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
author |
ROMERO TISOT, C. M. |
author_facet |
ROMERO TISOT, C. M. ZANELLA, R. SOUZA, A. P. LÖF, L. M. SOARES, J. C. M. MARQUES, M. G. ZANELLA, E. L |
author_role |
author |
author2 |
ZANELLA, R. SOUZA, A. P. LÖF, L. M. SOARES, J. C. M. MARQUES, M. G. ZANELLA, E. L |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
CÂNDIDA MALDANER ROMERO TISOT, UPF; RICARDO ZANELLA, UPF; UDESC/Lages; LUCAS MALLMANN LÖF, UPF; UFSM; MARIANA GROKE MARQUES, CNPSA; ERALDO LOURENSO ZANELLA, UPF. |
dc.contributor.author.fl_str_mv |
ROMERO TISOT, C. M. ZANELLA, R. SOUZA, A. P. LÖF, L. M. SOARES, J. C. M. MARQUES, M. G. ZANELLA, E. L |
dc.subject.por.fl_str_mv |
Melatonina Reprodução Animal Garanhão Criopreservação Espermatozóide Animal reproduction Stallions Melatonin Cryopreservation Spermatozoa Reactive oxygen species |
topic |
Melatonina Reprodução Animal Garanhão Criopreservação Espermatozóide Animal reproduction Stallions Melatonin Cryopreservation Spermatozoa Reactive oxygen species |
description |
Abstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2021-08-11T02:06:52Z 2021-08-11T02:06:52Z 2021-08-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Open Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133434 10.23880/oajvsr-16000202 |
identifier_str_mv |
Open Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020. 10.23880/oajvsr-16000202 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1133434 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1817695615061065728 |