Post-seminal development and cryopreservation of endemic or endangered bromeliads.

Detalhes bibliográficos
Autor(a) principal: SILVA, S. S. S.
Data de Publicação: 2021
Outros Autores: SOUZA, E. H., SOUZA, F. V. D., MAX, D. A. A. S., ROSSI, M. L., COSTA, M. A. P. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136440
Resumo: Vriesea bahiana, Hohenbergia castellanosii and Encholirium spectabile are endemic Brazilian species that are considered endemic or endangered. Development of strategies to conserve these species is important to prevent irreversible genetic erosion. The objective of this study was to evaluate the post-seminal development and seed cryopreservation of three endemic or in danger of extinction bromeliad species in Brazil, to obtain a protocol that can safeguard the genetic variability of these species. In the seed cryopreservation assay, we evaluated five desiccation periods. The seeds in the cryotubes were taken from the desiccator and immediately plunged into liquid nitrogen. For the analysis of post-seminal development, seeds in different germination stages were collected and evaluated by light and scanning electron microscopy. Vriesea bahiana seeds frozen in liquid nitrogen presented almost 100% germination, indicating dormancy break of this species. Vriesea bahiana can be cryopreserved with 5.9% water content after being dried for 24 hours. Hohenbergia castellanosii and E. spectabile seeds did not need to be desiccated before being cryopreserved. The most relevant morphological traits for differentiation of genera and subfamilies of Bromeliaceae are the shape and type of seed appendages. In this study, all three species presented well-differentiated size and shape of their structures.
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spelling Post-seminal development and cryopreservation of endemic or endangered bromeliads.CriopreservaçãoVriesea bahiana, Hohenbergia castellanosii and Encholirium spectabile are endemic Brazilian species that are considered endemic or endangered. Development of strategies to conserve these species is important to prevent irreversible genetic erosion. The objective of this study was to evaluate the post-seminal development and seed cryopreservation of three endemic or in danger of extinction bromeliad species in Brazil, to obtain a protocol that can safeguard the genetic variability of these species. In the seed cryopreservation assay, we evaluated five desiccation periods. The seeds in the cryotubes were taken from the desiccator and immediately plunged into liquid nitrogen. For the analysis of post-seminal development, seeds in different germination stages were collected and evaluated by light and scanning electron microscopy. Vriesea bahiana seeds frozen in liquid nitrogen presented almost 100% germination, indicating dormancy break of this species. Vriesea bahiana can be cryopreserved with 5.9% water content after being dried for 24 hours. Hohenbergia castellanosii and E. spectabile seeds did not need to be desiccated before being cryopreserved. The most relevant morphological traits for differentiation of genera and subfamilies of Bromeliaceae are the shape and type of seed appendages. In this study, all three species presented well-differentiated size and shape of their structures.SIMONE S.S. SILVA; EVERTON H. SOUZA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; DANIELA A.S. MAX; MONICA L. ROSSI; MARIA A. P. C. COSTA.SILVA, S. S. S.SOUZA, E. H.SOUZA, F. V. D.MAX, D. A. A. S.ROSSI, M. L.COSTA, M. A. P. C.2021-11-23T02:11:54Z2021-11-23T02:11:54Z2021-11-222021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnais da Academia Brasileira de Ciências, v.93, n.1, [2021?].1678-2690http://www.alice.cnptia.embrapa.br/alice/handle/doc/113644010.1590/0001-3765202120191133enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-11-23T02:12:04Zoai:www.alice.cnptia.embrapa.br:doc/1136440Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-11-23T02:12:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-11-23T02:12:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Post-seminal development and cryopreservation of endemic or endangered bromeliads.
title Post-seminal development and cryopreservation of endemic or endangered bromeliads.
spellingShingle Post-seminal development and cryopreservation of endemic or endangered bromeliads.
SILVA, S. S. S.
Criopreservação
title_short Post-seminal development and cryopreservation of endemic or endangered bromeliads.
title_full Post-seminal development and cryopreservation of endemic or endangered bromeliads.
title_fullStr Post-seminal development and cryopreservation of endemic or endangered bromeliads.
title_full_unstemmed Post-seminal development and cryopreservation of endemic or endangered bromeliads.
title_sort Post-seminal development and cryopreservation of endemic or endangered bromeliads.
author SILVA, S. S. S.
author_facet SILVA, S. S. S.
SOUZA, E. H.
SOUZA, F. V. D.
MAX, D. A. A. S.
ROSSI, M. L.
COSTA, M. A. P. C.
author_role author
author2 SOUZA, E. H.
SOUZA, F. V. D.
MAX, D. A. A. S.
ROSSI, M. L.
COSTA, M. A. P. C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv SIMONE S.S. SILVA; EVERTON H. SOUZA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; DANIELA A.S. MAX; MONICA L. ROSSI; MARIA A. P. C. COSTA.
dc.contributor.author.fl_str_mv SILVA, S. S. S.
SOUZA, E. H.
SOUZA, F. V. D.
MAX, D. A. A. S.
ROSSI, M. L.
COSTA, M. A. P. C.
dc.subject.por.fl_str_mv Criopreservação
topic Criopreservação
description Vriesea bahiana, Hohenbergia castellanosii and Encholirium spectabile are endemic Brazilian species that are considered endemic or endangered. Development of strategies to conserve these species is important to prevent irreversible genetic erosion. The objective of this study was to evaluate the post-seminal development and seed cryopreservation of three endemic or in danger of extinction bromeliad species in Brazil, to obtain a protocol that can safeguard the genetic variability of these species. In the seed cryopreservation assay, we evaluated five desiccation periods. The seeds in the cryotubes were taken from the desiccator and immediately plunged into liquid nitrogen. For the analysis of post-seminal development, seeds in different germination stages were collected and evaluated by light and scanning electron microscopy. Vriesea bahiana seeds frozen in liquid nitrogen presented almost 100% germination, indicating dormancy break of this species. Vriesea bahiana can be cryopreserved with 5.9% water content after being dried for 24 hours. Hohenbergia castellanosii and E. spectabile seeds did not need to be desiccated before being cryopreserved. The most relevant morphological traits for differentiation of genera and subfamilies of Bromeliaceae are the shape and type of seed appendages. In this study, all three species presented well-differentiated size and shape of their structures.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-23T02:11:54Z
2021-11-23T02:11:54Z
2021-11-22
2021
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Anais da Academia Brasileira de Ciências, v.93, n.1, [2021?].
1678-2690
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136440
10.1590/0001-3765202120191133
identifier_str_mv Anais da Academia Brasileira de Ciências, v.93, n.1, [2021?].
1678-2690
10.1590/0001-3765202120191133
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136440
dc.language.iso.fl_str_mv eng
language eng
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instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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