Post-seminal development and cryopreservation of endemic or endangered bromeliads.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136440 |
Resumo: | Vriesea bahiana, Hohenbergia castellanosii and Encholirium spectabile are endemic Brazilian species that are considered endemic or endangered. Development of strategies to conserve these species is important to prevent irreversible genetic erosion. The objective of this study was to evaluate the post-seminal development and seed cryopreservation of three endemic or in danger of extinction bromeliad species in Brazil, to obtain a protocol that can safeguard the genetic variability of these species. In the seed cryopreservation assay, we evaluated five desiccation periods. The seeds in the cryotubes were taken from the desiccator and immediately plunged into liquid nitrogen. For the analysis of post-seminal development, seeds in different germination stages were collected and evaluated by light and scanning electron microscopy. Vriesea bahiana seeds frozen in liquid nitrogen presented almost 100% germination, indicating dormancy break of this species. Vriesea bahiana can be cryopreserved with 5.9% water content after being dried for 24 hours. Hohenbergia castellanosii and E. spectabile seeds did not need to be desiccated before being cryopreserved. The most relevant morphological traits for differentiation of genera and subfamilies of Bromeliaceae are the shape and type of seed appendages. In this study, all three species presented well-differentiated size and shape of their structures. |
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Post-seminal development and cryopreservation of endemic or endangered bromeliads.CriopreservaçãoVriesea bahiana, Hohenbergia castellanosii and Encholirium spectabile are endemic Brazilian species that are considered endemic or endangered. Development of strategies to conserve these species is important to prevent irreversible genetic erosion. The objective of this study was to evaluate the post-seminal development and seed cryopreservation of three endemic or in danger of extinction bromeliad species in Brazil, to obtain a protocol that can safeguard the genetic variability of these species. In the seed cryopreservation assay, we evaluated five desiccation periods. The seeds in the cryotubes were taken from the desiccator and immediately plunged into liquid nitrogen. For the analysis of post-seminal development, seeds in different germination stages were collected and evaluated by light and scanning electron microscopy. Vriesea bahiana seeds frozen in liquid nitrogen presented almost 100% germination, indicating dormancy break of this species. Vriesea bahiana can be cryopreserved with 5.9% water content after being dried for 24 hours. Hohenbergia castellanosii and E. spectabile seeds did not need to be desiccated before being cryopreserved. The most relevant morphological traits for differentiation of genera and subfamilies of Bromeliaceae are the shape and type of seed appendages. In this study, all three species presented well-differentiated size and shape of their structures.SIMONE S.S. SILVA; EVERTON H. SOUZA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; DANIELA A.S. MAX; MONICA L. ROSSI; MARIA A. P. C. COSTA.SILVA, S. S. S.SOUZA, E. H.SOUZA, F. V. D.MAX, D. A. A. S.ROSSI, M. L.COSTA, M. A. P. C.2021-11-23T02:11:54Z2021-11-23T02:11:54Z2021-11-222021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAnais da Academia Brasileira de Ciências, v.93, n.1, [2021?].1678-2690http://www.alice.cnptia.embrapa.br/alice/handle/doc/113644010.1590/0001-3765202120191133enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2021-11-23T02:12:04Zoai:www.alice.cnptia.embrapa.br:doc/1136440Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542021-11-23T02:12:04falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542021-11-23T02:12:04Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Post-seminal development and cryopreservation of endemic or endangered bromeliads. |
title |
Post-seminal development and cryopreservation of endemic or endangered bromeliads. |
spellingShingle |
Post-seminal development and cryopreservation of endemic or endangered bromeliads. SILVA, S. S. S. Criopreservação |
title_short |
Post-seminal development and cryopreservation of endemic or endangered bromeliads. |
title_full |
Post-seminal development and cryopreservation of endemic or endangered bromeliads. |
title_fullStr |
Post-seminal development and cryopreservation of endemic or endangered bromeliads. |
title_full_unstemmed |
Post-seminal development and cryopreservation of endemic or endangered bromeliads. |
title_sort |
Post-seminal development and cryopreservation of endemic or endangered bromeliads. |
author |
SILVA, S. S. S. |
author_facet |
SILVA, S. S. S. SOUZA, E. H. SOUZA, F. V. D. MAX, D. A. A. S. ROSSI, M. L. COSTA, M. A. P. C. |
author_role |
author |
author2 |
SOUZA, E. H. SOUZA, F. V. D. MAX, D. A. A. S. ROSSI, M. L. COSTA, M. A. P. C. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
SIMONE S.S. SILVA; EVERTON H. SOUZA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; DANIELA A.S. MAX; MONICA L. ROSSI; MARIA A. P. C. COSTA. |
dc.contributor.author.fl_str_mv |
SILVA, S. S. S. SOUZA, E. H. SOUZA, F. V. D. MAX, D. A. A. S. ROSSI, M. L. COSTA, M. A. P. C. |
dc.subject.por.fl_str_mv |
Criopreservação |
topic |
Criopreservação |
description |
Vriesea bahiana, Hohenbergia castellanosii and Encholirium spectabile are endemic Brazilian species that are considered endemic or endangered. Development of strategies to conserve these species is important to prevent irreversible genetic erosion. The objective of this study was to evaluate the post-seminal development and seed cryopreservation of three endemic or in danger of extinction bromeliad species in Brazil, to obtain a protocol that can safeguard the genetic variability of these species. In the seed cryopreservation assay, we evaluated five desiccation periods. The seeds in the cryotubes were taken from the desiccator and immediately plunged into liquid nitrogen. For the analysis of post-seminal development, seeds in different germination stages were collected and evaluated by light and scanning electron microscopy. Vriesea bahiana seeds frozen in liquid nitrogen presented almost 100% germination, indicating dormancy break of this species. Vriesea bahiana can be cryopreserved with 5.9% water content after being dried for 24 hours. Hohenbergia castellanosii and E. spectabile seeds did not need to be desiccated before being cryopreserved. The most relevant morphological traits for differentiation of genera and subfamilies of Bromeliaceae are the shape and type of seed appendages. In this study, all three species presented well-differentiated size and shape of their structures. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-23T02:11:54Z 2021-11-23T02:11:54Z 2021-11-22 2021 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Anais da Academia Brasileira de Ciências, v.93, n.1, [2021?]. 1678-2690 http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136440 10.1590/0001-3765202120191133 |
identifier_str_mv |
Anais da Academia Brasileira de Ciências, v.93, n.1, [2021?]. 1678-2690 10.1590/0001-3765202120191133 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1136440 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
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Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
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EMBRAPA |
institution |
EMBRAPA |
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Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503512484216832 |