Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality.
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/937417 |
Resumo: | The trade creation and conservation of wild species in Amazon, including collared peccaries (Pecari tajacu), may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 °C over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates (n = 65) were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact (IPM) and sperm morphology. Selected ejaculates (n = 21) were diluted (1:1) in Beltsville Thawing Solution and kept during 48 hours under controlled temperature (17 °C). Assessments were made after dilution (T0), after 24 hours (T24), and 48 hours after the onset of cooling (T48). The storage impacted on sperm survival (P < 0.05). Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before. |
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Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality.SpermCollared peccarySemen conservationAmazonwildlifeThe trade creation and conservation of wild species in Amazon, including collared peccaries (Pecari tajacu), may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 °C over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates (n = 65) were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact (IPM) and sperm morphology. Selected ejaculates (n = 21) were diluted (1:1) in Beltsville Thawing Solution and kept during 48 hours under controlled temperature (17 °C). Assessments were made after dilution (T0), after 24 hours (T24), and 48 hours after the onset of cooling (T48). The storage impacted on sperm survival (P < 0.05). Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before.ALEXANDRE ROSSETTO GARCIA, CPPSEPRISCILA REIS KAHWAGE, UFPA/BELÉMDIVA ANELIE DE ARAÚJO GUIMARÃES, UNIVERSIDADE DO PARÁ/BELÉMOTÁVIO MITIO OHASHI, UNIVERSIDADE DO PARÁ/BELÉM.GARCIA, A. R.KAHWAGE, P. R.GUIMARÃES, D. A. de A.OSHASHI, O. M.2023-02-23T12:57:31Z2023-02-23T12:57:31Z2012-10-222012info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Agricultural Science and Technology A, v.2, p. 921-929, 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/937417enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2023-02-23T12:57:31Zoai:www.alice.cnptia.embrapa.br:doc/937417Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542023-02-23T12:57:31falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542023-02-23T12:57:31Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. |
title |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. |
spellingShingle |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. GARCIA, A. R. Sperm Collared peccary Semen conservation Amazon wildlife |
title_short |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. |
title_full |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. |
title_fullStr |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. |
title_full_unstemmed |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. |
title_sort |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. |
author |
GARCIA, A. R. |
author_facet |
GARCIA, A. R. KAHWAGE, P. R. GUIMARÃES, D. A. de A. OSHASHI, O. M. |
author_role |
author |
author2 |
KAHWAGE, P. R. GUIMARÃES, D. A. de A. OSHASHI, O. M. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
ALEXANDRE ROSSETTO GARCIA, CPPSE PRISCILA REIS KAHWAGE, UFPA/BELÉM DIVA ANELIE DE ARAÚJO GUIMARÃES, UNIVERSIDADE DO PARÁ/BELÉM OTÁVIO MITIO OHASHI, UNIVERSIDADE DO PARÁ/BELÉM. |
dc.contributor.author.fl_str_mv |
GARCIA, A. R. KAHWAGE, P. R. GUIMARÃES, D. A. de A. OSHASHI, O. M. |
dc.subject.por.fl_str_mv |
Sperm Collared peccary Semen conservation Amazon wildlife |
topic |
Sperm Collared peccary Semen conservation Amazon wildlife |
description |
The trade creation and conservation of wild species in Amazon, including collared peccaries (Pecari tajacu), may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 °C over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates (n = 65) were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact (IPM) and sperm morphology. Selected ejaculates (n = 21) were diluted (1:1) in Beltsville Thawing Solution and kept during 48 hours under controlled temperature (17 °C). Assessments were made after dilution (T0), after 24 hours (T24), and 48 hours after the onset of cooling (T48). The storage impacted on sperm survival (P < 0.05). Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-22 2012 2023-02-23T12:57:31Z 2023-02-23T12:57:31Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Agricultural Science and Technology A, v.2, p. 921-929, 2012. http://www.alice.cnptia.embrapa.br/alice/handle/doc/937417 |
identifier_str_mv |
Journal of Agricultural Science and Technology A, v.2, p. 921-929, 2012. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/937417 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503540079591424 |