Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.

Detalhes bibliográficos
Autor(a) principal: SILVA, G. R. da
Data de Publicação: 2018
Outros Autores: SOUZA, I. G. de B., PEREIRA, F. de M., SOUZA, B. de A., LOPES, M. T. do R., BENTZEN, P., DINIZ, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100712
Resumo: Brazilian native meliponines are currently threatened by increased human impacts. The assessment of their genetic variation by microsatellite DNA markers can assist in the conservation of populations and help in the planning and establishment of effi cient management strategies. The purpose of this study was to develop the fi rst set of microsatellite markers for Melipona fasciculata, selected from partial genome assembly of Illumina paired-end reads. Primer pairs were designed for each detected locus at their fl anking regions. Bee samples were genotyped from two different populations of Northeastern Brazil for marker characterization and validation...
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spelling Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.Abelha sem ferrãoMarcador MolecularHymenopteraApidaeBrazilian native meliponines are currently threatened by increased human impacts. The assessment of their genetic variation by microsatellite DNA markers can assist in the conservation of populations and help in the planning and establishment of effi cient management strategies. The purpose of this study was to develop the fi rst set of microsatellite markers for Melipona fasciculata, selected from partial genome assembly of Illumina paired-end reads. Primer pairs were designed for each detected locus at their fl anking regions. Bee samples were genotyped from two different populations of Northeastern Brazil for marker characterization and validation...GEICE RIBEIRO DA SILVA, UFPI; ISIS GOMES DE BRITO SOUZA, UFPI; FABIA DE MELLO PEREIRA, CPAMN; BRUNO DE ALMEIDA SOUZA, CPAMN; MARIA TERESA DO REGO LOPES, CPAMN; PAUL BENTZEN, Department of Biology, Dalhousie University, Halifax, NS, B3H 4J1, Canada; FABIO MENDONCA DINIZ, CNPC.SILVA, G. R. daSOUZA, I. G. de B.PEREIRA, F. de M.SOUZA, B. de A.LOPES, M. T. do R.BENTZEN, P.DINIZ, F. M.2018-12-05T00:00:23Z2018-12-05T00:00:23Z2018-12-0420182018-12-05T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEuropean Journal of Entomology, v. 115, p. 614-619, 2018.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100712enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2018-12-05T00:00:29Zoai:www.alice.cnptia.embrapa.br:doc/1100712Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542018-12-05T00:00:29falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542018-12-05T00:00:29Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.
title Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.
spellingShingle Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.
SILVA, G. R. da
Abelha sem ferrão
Marcador Molecular
Hymenoptera
Apidae
title_short Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.
title_full Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.
title_fullStr Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.
title_full_unstemmed Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.
title_sort Genome-wide discovery and characterization of microsatellite markers from Melipona fasciculata (Hymenoptera: Apidae), cross-amplifi cation and a snapshot assessment of the genetic diversity in two stingless bee populations.
author SILVA, G. R. da
author_facet SILVA, G. R. da
SOUZA, I. G. de B.
PEREIRA, F. de M.
SOUZA, B. de A.
LOPES, M. T. do R.
BENTZEN, P.
DINIZ, F. M.
author_role author
author2 SOUZA, I. G. de B.
PEREIRA, F. de M.
SOUZA, B. de A.
LOPES, M. T. do R.
BENTZEN, P.
DINIZ, F. M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv GEICE RIBEIRO DA SILVA, UFPI; ISIS GOMES DE BRITO SOUZA, UFPI; FABIA DE MELLO PEREIRA, CPAMN; BRUNO DE ALMEIDA SOUZA, CPAMN; MARIA TERESA DO REGO LOPES, CPAMN; PAUL BENTZEN, Department of Biology, Dalhousie University, Halifax, NS, B3H 4J1, Canada; FABIO MENDONCA DINIZ, CNPC.
dc.contributor.author.fl_str_mv SILVA, G. R. da
SOUZA, I. G. de B.
PEREIRA, F. de M.
SOUZA, B. de A.
LOPES, M. T. do R.
BENTZEN, P.
DINIZ, F. M.
dc.subject.por.fl_str_mv Abelha sem ferrão
Marcador Molecular
Hymenoptera
Apidae
topic Abelha sem ferrão
Marcador Molecular
Hymenoptera
Apidae
description Brazilian native meliponines are currently threatened by increased human impacts. The assessment of their genetic variation by microsatellite DNA markers can assist in the conservation of populations and help in the planning and establishment of effi cient management strategies. The purpose of this study was to develop the fi rst set of microsatellite markers for Melipona fasciculata, selected from partial genome assembly of Illumina paired-end reads. Primer pairs were designed for each detected locus at their fl anking regions. Bee samples were genotyped from two different populations of Northeastern Brazil for marker characterization and validation...
publishDate 2018
dc.date.none.fl_str_mv 2018-12-05T00:00:23Z
2018-12-05T00:00:23Z
2018-12-04
2018
2018-12-05T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv European Journal of Entomology, v. 115, p. 614-619, 2018.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100712
identifier_str_mv European Journal of Entomology, v. 115, p. 614-619, 2018.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1100712
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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