Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.

Detalhes bibliográficos
Autor(a) principal: GASPARI-PEZZOPANE, C. de
Data de Publicação: 2012
Outros Autores: BONTURI, N., GUERREIRO FILHO, O., FAVARIN, J. L., MALUF, M. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1044180
Resumo: The objective of this work was to identify genes that could be used as suitable markers for molecular recognition of phenological stages during coffee (Coffea arabica) fruit development. Four cultivars were evaluated as to their differential Expression of genes associated to fruit development and maturation processes. GEne expression was characterized by both semi?quantitative and quantitative RT?PCR, in fruit harvested at seven different developmental stages, during three different seasons. No size polymorphisms or differential expression were observed among the cultivars for the evaluated genes; however, distinct expression profiles along fruit development were determined for each gene. Four out of the 28 evaluated genes exhibited a regular expression profile in all cultivars and harvest seasons, and, therefore, they were validated as candidate phenological markers of coffee fruit. The gene ??galactosidase can be used as a marker of green stage, caffeine synthase as a marker of transition to green and yellowish?green stages, and isocitrate lyase and ethylene receptor 3 as markers of late maturation.
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spelling Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.Cup qualityDifferential expressionFruit phenologyQualidade de bebidaCoffea ArábicaRipeningThe objective of this work was to identify genes that could be used as suitable markers for molecular recognition of phenological stages during coffee (Coffea arabica) fruit development. Four cultivars were evaluated as to their differential Expression of genes associated to fruit development and maturation processes. GEne expression was characterized by both semi?quantitative and quantitative RT?PCR, in fruit harvested at seven different developmental stages, during three different seasons. No size polymorphisms or differential expression were observed among the cultivars for the evaluated genes; however, distinct expression profiles along fruit development were determined for each gene. Four out of the 28 evaluated genes exhibited a regular expression profile in all cultivars and harvest seasons, and, therefore, they were validated as candidate phenological markers of coffee fruit. The gene ??galactosidase can be used as a marker of green stage, caffeine synthase as a marker of transition to green and yellowish?green stages, and isocitrate lyase and ethylene receptor 3 as markers of late maturation.MIRIAN PEREZ MALUF, SAPC.GASPARI-PEZZOPANE, C. deBONTURI, N.GUERREIRO FILHO, O.FAVARIN, J. L.MALUF, M. P.2016-04-29T07:02:31Z2016-04-29T07:02:31Z2016-04-2820122016-04-29T07:02:31Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePesquisa Agropecuária Brasileira, v. 47, n. 7, p. 972-982, jul. 2012.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1044180enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T03:30:41Zoai:www.alice.cnptia.embrapa.br:doc/1044180Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T03:30:41falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T03:30:41Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
title Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
spellingShingle Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
GASPARI-PEZZOPANE, C. de
Cup quality
Differential expression
Fruit phenology
Qualidade de bebida
Coffea Arábica
Ripening
title_short Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
title_full Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
title_fullStr Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
title_full_unstemmed Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
title_sort Gene expression profile during coffee fruit development and identification of candidate markers for phenological stages.
author GASPARI-PEZZOPANE, C. de
author_facet GASPARI-PEZZOPANE, C. de
BONTURI, N.
GUERREIRO FILHO, O.
FAVARIN, J. L.
MALUF, M. P.
author_role author
author2 BONTURI, N.
GUERREIRO FILHO, O.
FAVARIN, J. L.
MALUF, M. P.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv MIRIAN PEREZ MALUF, SAPC.
dc.contributor.author.fl_str_mv GASPARI-PEZZOPANE, C. de
BONTURI, N.
GUERREIRO FILHO, O.
FAVARIN, J. L.
MALUF, M. P.
dc.subject.por.fl_str_mv Cup quality
Differential expression
Fruit phenology
Qualidade de bebida
Coffea Arábica
Ripening
topic Cup quality
Differential expression
Fruit phenology
Qualidade de bebida
Coffea Arábica
Ripening
description The objective of this work was to identify genes that could be used as suitable markers for molecular recognition of phenological stages during coffee (Coffea arabica) fruit development. Four cultivars were evaluated as to their differential Expression of genes associated to fruit development and maturation processes. GEne expression was characterized by both semi?quantitative and quantitative RT?PCR, in fruit harvested at seven different developmental stages, during three different seasons. No size polymorphisms or differential expression were observed among the cultivars for the evaluated genes; however, distinct expression profiles along fruit development were determined for each gene. Four out of the 28 evaluated genes exhibited a regular expression profile in all cultivars and harvest seasons, and, therefore, they were validated as candidate phenological markers of coffee fruit. The gene ??galactosidase can be used as a marker of green stage, caffeine synthase as a marker of transition to green and yellowish?green stages, and isocitrate lyase and ethylene receptor 3 as markers of late maturation.
publishDate 2012
dc.date.none.fl_str_mv 2012
2016-04-29T07:02:31Z
2016-04-29T07:02:31Z
2016-04-28
2016-04-29T07:02:31Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Pesquisa Agropecuária Brasileira, v. 47, n. 7, p. 972-982, jul. 2012.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1044180
identifier_str_mv Pesquisa Agropecuária Brasileira, v. 47, n. 7, p. 972-982, jul. 2012.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1044180
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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