Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products.
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062982 |
Resumo: | Abstract Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions. |
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Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products.Bee pathogensDetection of sporesApis Melliferahoneypollenroyal jellyAbstract Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions.Lubiane Guimarães-Cestaro, UFV; José Eduardo Serrão, UFV; Dejair Message, UFERSA; MARTA FONSECA MARTINS, CNPGL; Érica Weinstein Teixeira, APTA-SAA.GUIMARÃES-CESTARO, L.SERRÃO, J. E.MESSAGE, D.MARTINS, M. F.TEIXEIRA, É. W.2017-02-08T23:11:19Z2017-02-08T23:11:19Z2017-02-0720162017-02-08T23:11:19Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleJournal of Hymenoptera Research, n. 49, p. 43-50, 2016.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062982enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-16T04:08:51Zoai:www.alice.cnptia.embrapa.br:doc/1062982Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-16T04:08:51falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-16T04:08:51Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. |
title |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. |
spellingShingle |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. GUIMARÃES-CESTARO, L. Bee pathogens Detection of spores Apis Mellifera honey pollen royal jelly |
title_short |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. |
title_full |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. |
title_fullStr |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. |
title_full_unstemmed |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. |
title_sort |
Simultaneous detection of Nosema spp., Ascosphaera apis and Paenibacillus larvae in honey bee products. |
author |
GUIMARÃES-CESTARO, L. |
author_facet |
GUIMARÃES-CESTARO, L. SERRÃO, J. E. MESSAGE, D. MARTINS, M. F. TEIXEIRA, É. W. |
author_role |
author |
author2 |
SERRÃO, J. E. MESSAGE, D. MARTINS, M. F. TEIXEIRA, É. W. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Lubiane Guimarães-Cestaro, UFV; José Eduardo Serrão, UFV; Dejair Message, UFERSA; MARTA FONSECA MARTINS, CNPGL; Érica Weinstein Teixeira, APTA-SAA. |
dc.contributor.author.fl_str_mv |
GUIMARÃES-CESTARO, L. SERRÃO, J. E. MESSAGE, D. MARTINS, M. F. TEIXEIRA, É. W. |
dc.subject.por.fl_str_mv |
Bee pathogens Detection of spores Apis Mellifera honey pollen royal jelly |
topic |
Bee pathogens Detection of spores Apis Mellifera honey pollen royal jelly |
description |
Abstract Honey bees are responsible for pollinating many native and cultivated plant species. These insects can be affected by many pathogens, including fungi and bacteria, both of which can form spores that are easily dispersed within the colony by means of the stored products, among other routes. The objective of this study was to develop a method to detect spores of the honey bee pathogens Nosema apis, Nosema ceranae, Ascosphaera apis and Paenibacillus larvae in samples of honey, bee pollen and royal jelly. The method was standardized for each product individually, and then analyzed by monoplex and multiplex PCR, which showed the same detection thresholds: 1.25 spores/mL of honey for N. ceranae; 7.5 spores/mL of honey for A. apis; and 0.4 spore/mL of honey for P. larvae, respectively. The standardized technique was effective and rapid for the detection of these pathogens in bee products and can be used for the establishment of official methods of sanitary control of bee products, considering the growing national and international trade of these products and the movement or migration of colonies between regions. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-02-08T23:11:19Z 2017-02-08T23:11:19Z 2017-02-07 2017-02-08T23:11:19Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Hymenoptera Research, n. 49, p. 43-50, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062982 |
identifier_str_mv |
Journal of Hymenoptera Research, n. 49, p. 43-50, 2016. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1062982 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503431914782720 |