High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082688 |
Resumo: | Coffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdTexclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee?H. vastatrix interaction over a time course in susceptible and resistant coffee plants. |
id |
EMBR_8e16f5d91f8088866fe40520c336e223 |
---|---|
oai_identifier_str |
oai:www.alice.cnptia.embrapa.br:doc/1082688 |
network_acronym_str |
EMBR |
network_name_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository_id_str |
2154 |
spelling |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection.Coffee rustBiotrophic interactionTranscriptomeDisease resistanceCoffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdTexclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee?H. vastatrix interaction over a time course in susceptible and resistant coffee plants.Juan Carlos Florez, BIAGRO/BioCafé/UFV; Luciana Souto Mofatto, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas; Rejane do Livramento Freitas?Lopes, BIAGRO/BioCafé/UFV; Sávio Siqueira Ferreira, BIAGRO/BioCafé/UFV; Eunize Maciel Zambolim, BIAGRO/BioCafé/UFV; Marcelo Falsarella Carazzolle, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas; Laércio Zambolim, Departamento de Fitopatologia/Universidade Federal de Viçosa; EVELINE TEIXEIRA CAIXETA, SAPC.FLORES, J. C.MOFATTO, L. S.FREITAS-LOPES, R. do L.FERREIRA, S. S.ZAMBOLIM, E. M.CARAZZOLLE, M. F.ZAMBOLIM, L.CAIXETA, E. T.2017-12-15T23:19:15Z2017-12-15T23:19:15Z2017-12-1520172017-12-15T23:19:15Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePlant Molecular Biology, v. 95, n. 6, p. 607-623, Dec. 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082688enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-12-15T23:19:23Zoai:www.alice.cnptia.embrapa.br:doc/1082688Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-12-15T23:19:23falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-12-15T23:19:23Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection. |
title |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection. |
spellingShingle |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection. FLORES, J. C. Coffee rust Biotrophic interaction Transcriptome Disease resistance |
title_short |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection. |
title_full |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection. |
title_fullStr |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection. |
title_full_unstemmed |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection. |
title_sort |
High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection. |
author |
FLORES, J. C. |
author_facet |
FLORES, J. C. MOFATTO, L. S. FREITAS-LOPES, R. do L. FERREIRA, S. S. ZAMBOLIM, E. M. CARAZZOLLE, M. F. ZAMBOLIM, L. CAIXETA, E. T. |
author_role |
author |
author2 |
MOFATTO, L. S. FREITAS-LOPES, R. do L. FERREIRA, S. S. ZAMBOLIM, E. M. CARAZZOLLE, M. F. ZAMBOLIM, L. CAIXETA, E. T. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Juan Carlos Florez, BIAGRO/BioCafé/UFV; Luciana Souto Mofatto, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas; Rejane do Livramento Freitas?Lopes, BIAGRO/BioCafé/UFV; Sávio Siqueira Ferreira, BIAGRO/BioCafé/UFV; Eunize Maciel Zambolim, BIAGRO/BioCafé/UFV; Marcelo Falsarella Carazzolle, Laboratório de Genômica e Expressão/Departamento de Genética/Evolução e Bioagentes/Instituto de Biologia/Universidade Estadual de Campinas; Laércio Zambolim, Departamento de Fitopatologia/Universidade Federal de Viçosa; EVELINE TEIXEIRA CAIXETA, SAPC. |
dc.contributor.author.fl_str_mv |
FLORES, J. C. MOFATTO, L. S. FREITAS-LOPES, R. do L. FERREIRA, S. S. ZAMBOLIM, E. M. CARAZZOLLE, M. F. ZAMBOLIM, L. CAIXETA, E. T. |
dc.subject.por.fl_str_mv |
Coffee rust Biotrophic interaction Transcriptome Disease resistance |
topic |
Coffee rust Biotrophic interaction Transcriptome Disease resistance |
description |
Coffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdTexclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee?H. vastatrix interaction over a time course in susceptible and resistant coffee plants. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-15T23:19:15Z 2017-12-15T23:19:15Z 2017-12-15 2017 2017-12-15T23:19:15Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plant Molecular Biology, v. 95, n. 6, p. 607-623, Dec. 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082688 |
identifier_str_mv |
Plant Molecular Biology, v. 95, n. 6, p. 607-623, Dec. 2017. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1082688 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
_version_ |
1794503446585409536 |