Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.

Detalhes bibliográficos
Autor(a) principal: DINIZ, G. de F. D.
Data de Publicação: 2023
Outros Autores: COTA, L. V., FIGUEIREDO, J. E. F., LANA, U. G. de P., MARINS, M. S., SILVA, F. C., SANTOS, V. L. dos, OLIVEIRA-PAIVA, C. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162254
Resumo: Fungal diseases may cause significant damage to crops worldwide, generating yield losses, poor grain quality, and health risks to humans and animals. Biological control using antagonistic bacteria offers innovative solutions for sustainable management aiming at plant protection. However, beneficial plant-microorganism interactions are particular, and few antagonists with broad-spectrum activity have been reported. In this work, two bacteria isolated from sorghum seeds were identified by partial sequencing of the 16S rRNA gene and tested in vitro for their capacity to control six important pathogenic fungi of maize: Fusarium verticillioides, Macrophomina phaseolina, Stenocarpella sp., Fusarium graminearum, Colletotrichum graminicola, and Bipolaris sp. The molecular identification revealed that the bacterial isolates belong to the genera Bacillus (strain LIS05) and Paenibacillus (strain LIS04). Both bacterial isolates inhibited the growth of all six phytopathogens by at least 49%. The isolate LIS05 showed the most significant antagonistic potential against the fungal pathogens tested, at an average of 73% inhibition. The highest antagonist activity (86.1% inhibition) was observed in the confrontation test between the isolate LIS05 and C. graminicola. In addition to the mycelial growth inhibition, the isolate LIS04 blocked the production of dark pigments by Bipolaris sp. This study showed that LIS05 and LIS04 are promising alternatives for developing integrated management strategies to control fungal diseases in maize and sorghum
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spelling Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.Fungo fitopatogênicoBiocontroleMilhoZea MaysFungoPaenibacillusBacillus (bacteria)Fungal diseases may cause significant damage to crops worldwide, generating yield losses, poor grain quality, and health risks to humans and animals. Biological control using antagonistic bacteria offers innovative solutions for sustainable management aiming at plant protection. However, beneficial plant-microorganism interactions are particular, and few antagonists with broad-spectrum activity have been reported. In this work, two bacteria isolated from sorghum seeds were identified by partial sequencing of the 16S rRNA gene and tested in vitro for their capacity to control six important pathogenic fungi of maize: Fusarium verticillioides, Macrophomina phaseolina, Stenocarpella sp., Fusarium graminearum, Colletotrichum graminicola, and Bipolaris sp. The molecular identification revealed that the bacterial isolates belong to the genera Bacillus (strain LIS05) and Paenibacillus (strain LIS04). Both bacterial isolates inhibited the growth of all six phytopathogens by at least 49%. The isolate LIS05 showed the most significant antagonistic potential against the fungal pathogens tested, at an average of 73% inhibition. The highest antagonist activity (86.1% inhibition) was observed in the confrontation test between the isolate LIS05 and C. graminicola. In addition to the mycelial growth inhibition, the isolate LIS04 blocked the production of dark pigments by Bipolaris sp. This study showed that LIS05 and LIS04 are promising alternatives for developing integrated management strategies to control fungal diseases in maize and sorghumGISELE DE FÁTIMA DIAS DINIZ; LUCIANO VIANA COTA, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; MIKAELY SOUSA MARINS, UNIVERSIDADE FEDERAL DE LAVRAS; FELIPE CAMPOS SILVA, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI; VERA LÚCIA DOS SANTOS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS.DINIZ, G. de F. D.COTA, L. V.FIGUEIREDO, J. E. F.LANA, U. G. de P.MARINS, M. S.SILVA, F. C.SANTOS, V. L. dosOLIVEIRA-PAIVA, C. A.2024-02-26T13:34:50Z2024-02-26T13:34:50Z2024-02-262023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Milho e Sorgo, v. 22, e1342, 2023.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162254enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2024-02-26T13:34:50Zoai:www.alice.cnptia.embrapa.br:doc/1162254Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542024-02-26T13:34:50falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542024-02-26T13:34:50Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.
title Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.
spellingShingle Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.
DINIZ, G. de F. D.
Fungo fitopatogênico
Biocontrole
Milho
Zea Mays
Fungo
Paenibacillus
Bacillus (bacteria)
title_short Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.
title_full Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.
title_fullStr Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.
title_full_unstemmed Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.
title_sort Isolation and identification of broad-spectrum antagonist bacteria against pathogenic fungi of maize crop.
author DINIZ, G. de F. D.
author_facet DINIZ, G. de F. D.
COTA, L. V.
FIGUEIREDO, J. E. F.
LANA, U. G. de P.
MARINS, M. S.
SILVA, F. C.
SANTOS, V. L. dos
OLIVEIRA-PAIVA, C. A.
author_role author
author2 COTA, L. V.
FIGUEIREDO, J. E. F.
LANA, U. G. de P.
MARINS, M. S.
SILVA, F. C.
SANTOS, V. L. dos
OLIVEIRA-PAIVA, C. A.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv GISELE DE FÁTIMA DIAS DINIZ; LUCIANO VIANA COTA, CNPMS; JOSE EDSON FONTES FIGUEIREDO, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; MIKAELY SOUSA MARINS, UNIVERSIDADE FEDERAL DE LAVRAS; FELIPE CAMPOS SILVA, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI; VERA LÚCIA DOS SANTOS, UNIVERSIDADE FEDERAL DE MINAS GERAIS; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS.
dc.contributor.author.fl_str_mv DINIZ, G. de F. D.
COTA, L. V.
FIGUEIREDO, J. E. F.
LANA, U. G. de P.
MARINS, M. S.
SILVA, F. C.
SANTOS, V. L. dos
OLIVEIRA-PAIVA, C. A.
dc.subject.por.fl_str_mv Fungo fitopatogênico
Biocontrole
Milho
Zea Mays
Fungo
Paenibacillus
Bacillus (bacteria)
topic Fungo fitopatogênico
Biocontrole
Milho
Zea Mays
Fungo
Paenibacillus
Bacillus (bacteria)
description Fungal diseases may cause significant damage to crops worldwide, generating yield losses, poor grain quality, and health risks to humans and animals. Biological control using antagonistic bacteria offers innovative solutions for sustainable management aiming at plant protection. However, beneficial plant-microorganism interactions are particular, and few antagonists with broad-spectrum activity have been reported. In this work, two bacteria isolated from sorghum seeds were identified by partial sequencing of the 16S rRNA gene and tested in vitro for their capacity to control six important pathogenic fungi of maize: Fusarium verticillioides, Macrophomina phaseolina, Stenocarpella sp., Fusarium graminearum, Colletotrichum graminicola, and Bipolaris sp. The molecular identification revealed that the bacterial isolates belong to the genera Bacillus (strain LIS05) and Paenibacillus (strain LIS04). Both bacterial isolates inhibited the growth of all six phytopathogens by at least 49%. The isolate LIS05 showed the most significant antagonistic potential against the fungal pathogens tested, at an average of 73% inhibition. The highest antagonist activity (86.1% inhibition) was observed in the confrontation test between the isolate LIS05 and C. graminicola. In addition to the mycelial growth inhibition, the isolate LIS04 blocked the production of dark pigments by Bipolaris sp. This study showed that LIS05 and LIS04 are promising alternatives for developing integrated management strategies to control fungal diseases in maize and sorghum
publishDate 2023
dc.date.none.fl_str_mv 2023
2024-02-26T13:34:50Z
2024-02-26T13:34:50Z
2024-02-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Milho e Sorgo, v. 22, e1342, 2023.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162254
identifier_str_mv Revista Brasileira de Milho e Sorgo, v. 22, e1342, 2023.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1162254
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
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instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
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reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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