Effect of 2,4-d on callus induction in leaf explants of peach palm (bactris gasipaes h.b.k.).

• Autor(a) principal: SANTOS, M. R. A. dos
• Data de Publicação: 2016
• Outros Autores: PAZ, E. S.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
• Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1057485
• topic: Peach palm; Callogenesis; Dichlorophenoxyacetic acid; Pessegueiro; Ácido diclorofenoxiacético; Calogênese; Arecaceae

The objective of this research was to determine the effect of dichlorophenoxyacetic acid (2,4-D) callus in peach friable calluses in leaf explants of Bactris gasipaes H.B.K., aiming for further induction of somatic embryos. The explants were inoculated in MS medium supplemented with 30.0 g.L-1 sucrose, 8.0 g.L-1 agar and 2,4-D (0.0, 0.31, 0.62, 1.25, 2.5, 5.0, 10.0, and 20.0 mg.L-1). The cultures were kept in a growth room at 24±2°C under light conditions (50 µmol.m-2.s-1, photoperiod of 16 hours), and also under dark conditions, in factorial arrangement: 2 (light and dark conditions) x 8 (2,4-D concentrations), totaling 16 treatments. On the 14th day of cultivation, occurrences of oxidation and necrosis of the explants were observed and, on the 35th day, the formation of friable calluses, and the percentage of explant area covered by callus cells (EACC) were evaluated. Oxidation was more intense in the cultivations under light conditions, and reduced as the 2,4-D concentrationsincreased. However, the highest concentration, 20.0 mg.L-1, caused necrosis of the explants. The concentration of 10 mg.L-1 was the most efficient for friable callus induction reaching all the explants in the cultures kept in the dark. This concentration also resulted in the highest EACC, with an average of 56.5% of the explant area covered by callus cells.